The Fine Structure of the Oocyte in the Mouse Ovary Studied With Electron Microscope

Yamada, Eichi; Muta, Toyoko; Motomura, Akira; Koga, Hidetaka

doi:10.2739/kurumemedj.4.148

Cited by 85 publications

(24 citation statements)

References 15 publications

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“…Somewhat similar aggregates have been described in the mouse oocyte by Yamada et al (1957) and Rhodin (1963) and in rat oocytes at much later developmental stages by both Odor (1960), who called them a "vesicular complex," and Sotelo and Porter (1959) who noted their resemblance to similar concentrations of vesicles in the centrosphere region of somatic cells. Sotelo and Porter postulated that these clumps of vesicles in mature or fertilized rat ova are derived from earlier accumulations of vesicles liberated from muhivesicular bodies.…”

mentioning

confidence: 71%

“…The reports of Sotelo and Porter (1959) and Odor (1960) on rat oocytes, Yamada et al (1957) on mouse oocytes, Blanchette (1961) on rabbit oocytes, and Wartenburg and Stegner (1960) on human oocytes have indicated that endoplasmic reticulum is present only in meager amounts. In contrast, we find in guinea pig oocytes the development, by the primary follicle stage, of a complex ergastoplasm.…”

Section: Endoplasmic Reticulum Appears As a Variety Ofmentioning

confidence: 99%

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Studies on Guinea Pig Oocytes

Adams

Hertig

1964

The Journal of Cell Biology

145

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Oocytes in primordial and primary follicles of young adult guinea pig ovaries fixed in osmium tetroxide and embedded in Epon 812, have been observed by electron microscopy. The gradual differentiation of a series of cytoplasmic organelles has been correlated with the growth in size of the oocyte and the development of the follicular wall. The most immature prim~)rdial oocyte is morphologically a simple cell consisting of a large slightly eccentric nucleus, a few large spherical mitochondria, a profusion of granular cytoplasmic vesicles, and free RNP particles. At the primary follicle stage, abundant endoplasmic reticulum, clusters of mitochondria proliferating around a rosette formation, multiple Golgi complexes, vesicular aggregates forming cortical granules, and a profusion of microvilli have been differentiated. Concentrations of cytoplasmic organelles at the periphery of the oocyte in the primary follicle suggest that it is equipped for the absorption, utilization, and intracellular transport of material delivered to its surface membrane. The juxtaposition of components of the ultrastructure during the development and growth of this large cell appears to follow a precise pattern and provides an unusual opportunity to study the interrelationships of differentiating organelles.

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mentioning

confidence: 71%

Section: Endoplasmic Reticulum Appears As a Variety Ofmentioning

confidence: 99%

Studies on Guinea Pig Oocytes

Adams

Hertig

1964

The Journal of Cell Biology

145

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“…According to some investigators the zona pellucida originates from the oocyte (13,14), whereas others feel that it originates from the follicle cells (24,25) or from both the oocyte and the follicle cells (26,27). The (29).…”

Section: Discussionmentioning

confidence: 99%

Synthesis of zona pellucida proteins by denuded and follicle-enclosed mouse oocytes during culture in vitro

Bleil

Wassarman

1980

Proc. Natl. Acad. Sci. U.S.A.

193

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ABSTRACTlucida proteins during culture in vitro, whereas zonae pellucidae removed from fully-grown oocytes (85 gm) were not radiolabeled to a significant extent. After culture of denuded or follicleenclosed oocytes for 12 hr, more than 95% of the [3Hlfucose incorporated into oocyte proteins was found in ZP1, ZP2, and ZP3, indicating that the zona pellucida proteins are the major class of proteins glycosylated during oocyte growth. These results provide biochemical evidence supporting the idea that the zona pellucida originates from the mammalian oocyte itself, rather than from the surrounding follicle cells.The zona pellucida is a relatively thick, translucent, acellular coat that surrounds the plasma membrane of fully grown mammalian oocytes and performs a variety of vital biological functions during early mammalian development. The zona pellucida is laid down during growth of the ovarian follicle, remains throughout preimplantation development, and is finally shed as the blastocyst readies for implantation (1-3). Several lines of evidence suggest that the zona pellucida possesses a receptor for sperm, that it acts as a barrier to further sperm penetration after fertilization, and that its presence is necessary for normal early development in vivo (3-5). In certain respects the zona pellucida is morphologically and functionally analogous to the vitelline envelope that surrounds oocytes of lower animal species (6, 7).The site of origin of the zona pellucida has been the subject of considerable interest for more than a century (8-10). DespiteThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. 1029 numerous morphological, ultrastructural, histological, and autoradiographic studies, it has remained an unresolved issue as to whether the zona pellucida originates from the oocyte, follicle cells, or both (11)(12)(13)(14).Recently, we identified and characterized those proteins that compose the mouse oocyte's zona pellucida (15,16). It has been known for some time, primarily on the basis of its staining properties, that the zona pellucida contains protein and carbohydrate (3, 5), and our recent work demonstrated that the zona pellucida is composed of three different protein species, which we have designated ZP1, ZP2, and ZP3. Having identified the proteins that compose the zona pellucida has permitted us to examine the site of synthesis of these proteins during follicular development. In this report we provide biochemical evidence supporting the idea that the zona pellucida originates from the oocyte itself. MATERIALS AND METHODS Collection and Culture of Denuded and Follicle-EnclosedOocytes. Ovaries were excised from 8 to 15-day-old Swiss albino mice (CD-1, Charles River Breeding Laboratories), placed into standard egg culture medium (SECM) (17), and then punctured with fine steel needles in order to obtain denuded oocytes (i.e., free of follicle cells by morph...

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“…The findings of Blandan and Odor (1972) seem to indicate that granulosa cells are directly involved in the fabrication of the zona pellucida. There is very little evidence to support the other theory that zona formation is attributed to the participation of the oocyte and granulosa cells (Yamada et al, 1957;Hadek, 1965;Hope, 1965). Nevertheless Wartenberg and Stegner (1960) suggested that the outer part of zona pellucida which is secreted by the follicular cells consists of acid mucopolysaccharide, while the inner portion originating from the oocyte is composed of neutral mucopolysaccharide.…”

Section: Discussionmentioning

confidence: 78%

Carbohydrate Histochemistry of Ovarian Follicle With Reference to the Formation of Zona Pellucida in Rabbit

Verma

1980

Acta Histochem. Cytochem

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The Fine Structure of the Oocyte in the Mouse Ovary Studied With Electron Microscope

Cited by 85 publications

References 15 publications

Studies on Guinea Pig Oocytes

Studies on Guinea Pig Oocytes

Synthesis of zona pellucida proteins by denuded and follicle-enclosed mouse oocytes during culture in vitro

Carbohydrate Histochemistry of Ovarian Follicle With Reference to the Formation of Zona Pellucida in Rabbit

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