The fine structure of ascospore delimitation in the yeast <i>Wickerhamia fluorescens</i>

Rooney, Lynn; Møens, Peter B.

doi:10.1139/m73-224

Cited by 11 publications

(6 citation statements)

References 4 publications

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“…The broad outline of the cytological steps of spore construction in S. cerevisiae and the overall similarity of sporulation in a variety of other ascomycetes were established by a plethora of early electron microscopy studies (7,8,22,50,52,91,99,128,136,139). Those studies firmly established the enclosure of the nucleus within a double membrane and the subsequent formation of the spore wall within the lumens of those membranes as the central features of this cell division event.…”

Section: Perspectives and Future Directionsmentioning

confidence: 96%

Ascospore Formation in the YeastSaccharomyces cerevisiae

Neiman

2005

Microbiol Mol Biol Rev

216

237

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SUMMARY Sporulation of the baker's yeast Saccharomyces cerevisiae is a response to nutrient depletion that allows a single diploid cell to give rise to four stress-resistant haploid spores. The formation of these spores requires a coordinated reorganization of cellular architecture. The construction of the spores can be broadly divided into two phases. The first is the generation of new membrane compartments within the cell cytoplasm that ultimately give rise to the spore plasma membranes. Proper assembly and growth of these membranes require modification of aspects of the constitutive secretory pathway and cytoskeleton by sporulation-specific functions. In the second phase, each immature spore becomes surrounded by a multilaminar spore wall that provides resistance to environmental stresses. This review focuses on our current understanding of the cellular rearrangements and the genes required in each of these phases to give rise to a wild-type spore.

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Section: Perspectives and Future Directionsmentioning

confidence: 96%

Ascospore Formation in the YeastSaccharomyces cerevisiae

Neiman

2005

Microbiol Mol Biol Rev

216

237

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“…One or two spores developed in the ascus; besides a "non-functional nucleus" as described by Rooney and Moens (1973) was sometimes visible. It probably degenerated.…”

Section: Resultsmentioning

confidence: 99%

“…However, a lobed nucleus and an incomplete prospore wall were observed in the mother cell which suggests that meiosis occurs in this cell and not in the bud as proposed by Ferreira and Phaff (1959). As in Wickerhamia fluorescens (Rooney and Moens, 1973) and in Debaryomyces hansenii (Kregervan Rij and Veenhuis, 1975), only one or two spores are formed and part of the nucleus remains unused.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopy of sporulation inSchwanniomyces alluvius

Kregervanrij¹

1977

Antonie van Leeuwenhoek

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Sporulation in Schwanniomyces alluvius appeared to be preceded by fusion of a mother and daughter cell. Meiosis probably occurred in the mother cell and one or two spores were formed in the latter. A study of thin sections showed that the spore wall developed from a prospore wall. The mature spore wall consisted of a broad light inner layer and a thinner dark outer layer including warts. An equatorial ledge was present. During germination in the ascus, a new light inner layer was formed and the old layers of the spore wall partly broke up. Ascospores in a strain of S. persoonii had a different wall structure in that the dark layer had changed into light areas separated by dark material which formed bulges at the surface.

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“…In the two yeasts meiosis I results in a single bilobed nucleus and during nleiosis I1 there are two spindles within a complex nucleus. Also, the spindle-pole bodies appear to be the spore wall organizing centers in both yeasts (18).…”

Section: Discussionmentioning

confidence: 98%

Nuclear divisions at meiosis in the ascomycetous yeast Wickerhamia fluorescens

Rooney¹,

Møens²

1973

Can. J. Microbiol.

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ROONEY, L., and P. B. MOENS. 1973. Nuclear divisions at meiosis in the ascomycetous yeast Wickerfiarnia juoresceris. Can. J. Microbiol. 19: 1383-1387. In cultures of cells which have been on sporulation medium for 22 h, all stages of meiosis and sporulation occur. In electron microscopy of glutaraldehyde-fixed cells, spindles and spindle-pole bodies are preserved but chromosomes and kinetochores are not apparent. Photographic records of complete serial sections of some 40 asci in different developmental stages show that during the first meiotic division, the nuclear envelope remains intact and the spindle elongates from about 1 pm to 4 pm. Each of the spindle-pole bodies then divides and the bilobed nucleus contains two short spindles (one in each lobe). During the second division the spindles elongate from about 0.5 pm to 3.5 pm while the nuclear envelope remains intact. As a result there is a four-lobed nucleus, either in a chain formation or in a more compact arrangement. The spindle-pole bodies increase in complexity to varying degrees during the second division. One becomes the organizer of a complete ascospore wall while the other three may develop an inco~nplete wall and eventually these three nuclei degenerate in the cytoplasm of the ascus. It is shown that the nuclear behavior during meiosis parallels that of the yeast Sacchar.or7zyces cermisiae but differs from that reported for Euascomycetes. ROONEY, L., et P. B. MOENS. 1973. Nuclear divisions at meiosis in the ascomycetous yeast Wicker.harnia juorescens. Can. J. Microbiol. 19: 1383-1387. Dans des cultures de cellules qui ont sejournk 22 h dans un milieu a sporulation, on retrouve tous les stades de la mti'ose et de la sporulation. Lors de I'examen au microscope Clectronique des cellules fixees a la glutaraldehyde, les fuseaux et les corps polaires fusiformes sont intacts, mais les chromosomes et les cinttochores sont inapparents. L'observation photographique de toute une skrie de coupes de 40 asques prises a divers stades du developpement fait voir que, pendant la premiere division mti'otique, I'enveloppe nucleaire demeure intacte et le fuseau s'allonge de 1 pm a 4 pm. Chacun des corps polaires fusiformes se divise et le noyau bilobe contient deux courts fuseaux (un dans chaque lobe). Pendant la seconde division, les fuseaux s'allongent de 0.5 p n~ 2 3.5 11n1 tandis que I'enveloppe nucleaire demeure intacte.Comme resultat, on retrouve un noyau a quatre lobes en chainette ou en amas plus compact. Pendant la seconde division, les corps polaires deviennent plus complexes a divers degres. Un noyau devient I'initiateur d'une paroi complete de I'ascospore tandis que les trois autres peuvent causer un developpement d'une paroi incon~plete; eventuellement ces trois noyaux digenerent dans le cytoplasme de l'asque. I1 est demontre que le comportenlent nucliaire lors de la mti'ose va de pair avec celui de Snccharomyces cerevisiae mais diffkre de ce qui est rapport6 pour les Euascomycetes.[Traduit par le journal]

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The fine structure of ascospore delimitation in the yeast Wickerhamia fluorescens

Cited by 11 publications

References 4 publications

Ascospore Formation in the YeastSaccharomyces cerevisiae

Ascospore Formation in the YeastSaccharomyces cerevisiae

Electron microscopy of sporulation inSchwanniomyces alluvius

Nuclear divisions at meiosis in the ascomycetous yeast Wickerhamia fluorescens

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