The fine structure and development of the peritubular contractile cell component in the seminiferous tubules of the mouse

Ross, Michael H.

doi:10.1002/aja.1001210307

Cited by 117 publications

(51 citation statements)

References 18 publications

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“…Hydrocarbons from cigarette smoke may also interact with speci®c genetic factors, such as those of hydrocarbon metabolism, DNA repair and genetic variability [25], which are likely to be important only for normal reproductive function. A possible effect on the genome from tobacco has been suggested [26].…”

Section: Discussionmentioning

confidence: 99%

Nicotine toxicity to the ultrastructure of the testis in rats

Aydos¹,

Güven²,

Can³

et al. 2001

BJU International

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Objective To investigate the in¯uence of nicotine exposure on the ultrastructure of the rat testis. Materials and methods Twenty rats were injected with nicotine at a dose of 0.4 mg/100 g body weight daily for 3 months; a group of 20 control rats matched for weight and age were injected with saline only for the same duration. The testes were then harvested and examined by transmission electron microscopy. Results Rats given nicotine showed: thickening of the tunica propria, caused by an increase in the collagen ®bres under the irregular basal lamina; degeneration of junctional specializations between the Sertoli cells, with malformed nuclei showing condensed chromatin; Sertoli cells with numerous polymorphic mitochondria with irregular cristae and an electron-dense matrix. The germ cells were degenerated, spermatids retained excess cytoplasm and accumulated electrondense lipid droplets in the cytoplasm. The acrosomes were irregular and abnormally con®gured. Conclusion There were ultrastructural alterations in rats exposed to nicotine that could be attributed to the detrimental effects of nicotine on germ cells, peritubular structures and Sertoli cells.

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Section: Discussionmentioning

confidence: 99%

Nicotine toxicity to the ultrastructure of the testis in rats

Aydos¹,

Güven²,

Can³

et al. 2001

BJU International

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“…This is due in part to the complicated texture of the lamina propria of seminiferous tubules in the human testis. In the rat testis the peritubular tissue consists of only one layer of myoid cells, and therefore has been well investigated (CLERMONT, 1958;LACY and ROTBLAT, 1960;LEESON and LEESON, 1963;Ross, 1967;TOYAMA, 1977;VIRTANEN et al, 1986;MAEKAWA et al, 1991MAEKAWA et al, , 1995. The myofibroblasts of the ratt seminiferous tubules possess bundles of filaments (stress fibres) and express the cytoskeletal proteins desmin, a-smooth muscle actin and smooth muscle myosin (SKALLI et al, 1986;BENZONANA et al, 1988;SAPPING et al, 1990, for review).…”

Section: Discussionmentioning

confidence: 99%

“…This unique cell shape of a myofibroblast surrounding a human seminiferous tubule differs markedly from other myofibroblasts in the human genital tract, e. g. in the coat of the ductuli efferentes or of the ductus epididymidis (HOLSTEIN, 1969), in the tunica dartos (HOLSTEIN et al, 1974) and from testicular peritubular myoid cells of other species excluding some higher vertebrates and primates (CLERMONT, 1958;LACY and ROTBLAT, 1960;LEESON and LESSON, 1963;ROSS, 1967;CHRISTL, 1990;MAEKAWA et al, 1991;PALOMBI et al, 1992). The myoid nature of the cells of the lamina propria of the guinea pig, rat and human seminif erous tubules was earlier proved (GROSCHEL-STEWARD and UNSICKER, 1977;VIRTANEN et al, 1986;SKALLI et al, 1986;BENZONANA et al, 1988).…”

Section: Myofibroblastsmentioning

confidence: 93%

Myofibroblasts in the Lamina Propria of Human Seminiferous Tubules are Dynamic Structures of Heterogeneous Phenotype.

Holstein

Maekawa

Nagano

et al. 1996

Archives of Histology and Cytology

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“…Morphological changes in the tubules include the mitotic quiescence of the primordial germ cells (PGCs), the proliferation of the supporting cells, the formation of well-organized single cell layer of the Sertoli cells along the basement membrane, the reinitiation of the gonocyte mitosis, the movement of the gonocyte to the basement membrane, and the differentiation of the PGCs into the spermatogonia [1, 3,17,18,20,27]. In the interstitial tissue, the differentiation of the peritubular myoid cells, the maturation of the basement membrane [14,23,26], and the appearance of adult type Leydig cells [13] occurred in the developing testis. These events would be regulated by extratesticular factors and various type of cellcell and cell-matrix interactions in developing testis.…”

Section: Animalsmentioning

confidence: 99%

Temporal and Spatial Distribution of Alkaline Phosphatase Activity in Male Hypogonadic Rat (hgn/hgn) Testis during Postnatal Development.

Suzuki

Inaba

Suzuki

1998

The Journal of Veterinary Medical Science

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ABSTRACT. In male hypogonadic mutant rat (hgn/hgn), gonocytes degenerate and peritubular cells form multiple layers around seminiferous tubules during early postnatal testicular development. Alkaline phosphatase (AP) activity has been used as not only a tracer for the primordial germ cells (PGCs) but also a histochemical marker for the peritubular myoid cells. In the present study, we examined the localization of AP activity during the postnatal testicular development in the hgn/hgn and phenotypically normal (+/+ or +/hgn) rat. In the normal testis, high AP activity was located in the surface of the PGCs on 3 days of age. As the PGCs differentiated into spermatogonia, the AP activity drastically decreased in intensity on 7 days and was completely lost by 12 days. In the hgn/hgn, the PGCs showing high AP activity occupied the inside of dilated seminiferous tubules on 3 and 7 days of age. The luminal AP activity declined gradually by 18 days and disappeared on 21 days, when the germ cells completely degenerated in the hgn/hgn testis. In the normal, high AP activity emerged in a layer of the peritubular cells surrounding the tubules on 7 days and afterwards, indicating that the peritubular cells were differentiating into myoid cells. In the hgn/hgn, the peritubular cells formed multiple layers around the tubules and showed weak AP activity on 3 to 18 days of age. On 21 days of age, high AP activity emerged in a single layer of the peritubular cells directly attached to the basement membrane in the hgn/hgn. These results indicate that the PGCs showing AP activity kept remained at later stage in the hgn/ hgn and that the single layer of mature myoid cells showing high AP activity appeared much later in the hgn/hgn testis than in normal. -KEY WORDS: alkaline phosphatase, hypogonadism, peritubular myoid cell, primordial germ cell, testicular development.J. Vet. Med. Sci. 60(6): 671-679, 1998 cells, the abnormal cell division of the gonocytes, the degeneration of the gonocytes, and the formation of multiple layers of the peritubular cells around the seminiferous tubules [32]. Alkaline phosphatases (APs) are membranelinked enzymes that hydrolyze orthophosphate monoesters at alkaline pH [4]. In normal murine testis, AP activity is predominantly localized in the gonocytes and the myoid cells [2,11,16,22]. In this study, we examine the temporal and spatial distribution of AP activity in the postnatal hgn/ hgn and normal (+/+ or hgn/hgn) testis to investigate the distribution and development of the hgn/hgn gonocytes and peritubular myoid cells. MATERIALS AND METHODS Animals:Male normal and hgn/hgn rats used in this study were obtained from matings between proven +/hgn males and females derived from the 21st generation of hypogonadic rat (HGN) line maintained in our department [29]. The rats were kept in a conventional animal room, and were fed a mixed grain diet and water ad libitum [31].Preparation of cryosectioned testis samples: The rats were sacrificed on 3, 7, 12, 18, 21, and 40 days after birth by overdose ether. Th...

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The fine structure and development of the peritubular contractile cell component in the seminiferous tubules of the mouse

Cited by 117 publications

References 18 publications

Nicotine toxicity to the ultrastructure of the testis in rats

Nicotine toxicity to the ultrastructure of the testis in rats

Myofibroblasts in the Lamina Propria of Human Seminiferous Tubules are Dynamic Structures of Heterogeneous Phenotype.

Temporal and Spatial Distribution of Alkaline Phosphatase Activity in Male Hypogonadic Rat (hgn/hgn) Testis during Postnatal Development.

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