The fine‐structural changes observed in the rectal papillae of the mosquito <i>Aedes aegypti, L.</i> and their relation to the epithelial transport of water and inorganic ions

Cr, Hopkins

doi:10.1111/j.1365-2818.1967.tb00585.x

Cited by 59 publications

(18 citation statements)

References 25 publications

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“…Both the rectal pads and the anterior hindgut possess a well-developed basolateral labyrinth populated with mitochondria, a trait shared with the larval rectum and anal papillae (Clements, 2000). Following a blood meal, the most notable changes occurred in the basal and lateral membrane systems of the rectal pads, intimating enhanced ion and water absorption activity (Hopkins, 1967). In Fig.·4C, the intense P-type Na + /K + -ATPase labeling on these basolateral infoldings of the rectal pads indicate that the Na + pump must be driving these absorptive processes following a blood meal.…”

Section: Hindgut and Larval Anal Papillaementioning

confidence: 99%

“…The apical membrane of the anterior hindgut contains elaborate infoldings with mitochondria (Tongu et al, 1969) and was found to highly express the H + pump (Fig.·4E,F). By contrast, Hopkins (Hopkins, 1967), reported that the apical membrane of the rectal pads is not elaborately infolded. We observed only a thin layer of V-type H + -ATPase expression in this region (Fig.·4C,D) thus confirming that this membrane is not extensive.…”

Section: Hindgut and Larval Anal Papillaementioning

confidence: 99%

“…Our study is the first to examine specific ion transport proteins in these epithelia. The patterns of basolateral P-type Na + /K + -ATPase and apical V-type H + -ATPase localization in the adult hindgut (Fig.·4), in addition to previous studies (Hopkins, 1967;Tongu et al, 1969) infer that both the anterior hindgut and rectal pads are sites of ion and water movement. The rectum of adult A. aegypti consists of a thin rectal sac in which six (female) or four (male) rectal pads (Clements, 2000) protrude into the lumen (Fig.·4A-D).…”

Section: Hindgut and Larval Anal Papillaementioning

confidence: 99%

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P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquitoAedes aegypti

Patrick

Aimanova

Sanders

et al. 2006

Journal of Experimental Biology

162

174

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SUMMARY This study describes the expression patterns of P-type Na+/K+-ATPase and V-type H+-ATPase in the larval and adult forms of the mosquito Aedes aegypti and provides insight into their relative importance in ion transport function of key osmoregulatory organs. RT-PCR assays indicate that, at the level of the gene,both ATPases are expressed in all of the osmoregulatory tissues of larvae(midgut, Malpighian tubules, rectum and anal papillae) and adults (stomach,Malpighian tubules, anterior hindgut and rectum). Immunohistochemical studies determined that both ATPases are present in high levels in all the relevant organs, with the exception of the larval rectum (P-type Na+/K+-ATPase only). In larval gastric caeca, ATPase location corresponds to the secretory (basal P-type Na+/K+-ATPase, apical V-type H+-ATPase) and ion-transporting (V-type H+-ATPase on both membranes) regions as previously described. The two ATPases switch membrane location along the length of the larval midgut, indicating three possible regionalizations,whereas the adult stomach has uniform expression of basolateral P-type Na+/K+-ATPase and apical V-type H+-ATPase in each cell. In both larval and adult Malpighian tubules, the distal principal cells exhibit high expression levels of V-type H+-ATPase (apically and cytoplasmically) whereas P-type Na+/K+-ATPase is highly expressed in stellate cells found only in the distal two-thirds of each tubule. By contrast, the proximal principal cells express both P-type Na+/K+-ATPase (basal) and V-type H+-ATPase(apical). These results suggest a functional segregation along the length of the Malpighian tubules based on cell type and region. P-type Na+/K+-ATPase is the only pump apparent in the larval rectum whereas in the larval anal papillae and the adult hindgut (including the anterior hindgut and rectum with rectal pads), P-type Na+/K+-ATPase and V-type H+-ATPase localize to the basal and apical membranes, respectively. We discuss our findings in light of previous physiological and morphological studies and re-examine our current models of ion transport in these two developmental stages of mosquitoes that cope with disparate osmoregulatory challenges.

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Section: Hindgut and Larval Anal Papillaementioning

confidence: 99%

Section: Hindgut and Larval Anal Papillaementioning

confidence: 99%

Section: Hindgut and Larval Anal Papillaementioning

confidence: 99%

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P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquitoAedes aegypti

Patrick

Aimanova

Sanders

et al. 2006

Journal of Experimental Biology

162

174

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“…Baccetti, 1962;Gupta & Berridge, 1966;Hopkins, 1967;Judy & Gilbert, 1970;Wessing & Eichelberg, 1973;Wall & Oschman, 1975;Dallai et al, 1985).…”

Section: Introductionmentioning

confidence: 92%

Ultrastructural organization of the rectal pads in adultStenophylax permistusMcL. (Trichoptera)

Cianficconi

Sorcetti

Moretti

et al. 1985

Bolletino di zoologia

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The epithelium of the rectal sac in S. permistirs is organized to form numerous circular pads. Each pad consists of about ten large cells covered by a cuticle and surrounded by sheath cells that form a barrier isolating the rectal pad. The largest cells (cortical cells), with long microviIIi in the apical region and numerous structural complexes formed by the association between the lateral plasma membrane and mitochondria, are engaged actively in drawing fluids from the gut lumen. Subcuticular areas are intermingled with the microvilli. Beneath these cells is an infundibular space delimited by a continuous layer of cells (medullary cells) with a structural organization suggestive of their participation in ion regulation. ACKNOWLEDGEMENTS

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“…The ultrastructure of the rectal pads has been studied in a number of insects: Dictyoptera by Oschman and Wall, (1969), Wall and Oschman (1973) and by Noirot and Noirot-Timoth~e (1976); Isoptera by Noirot and Noirot-Timoth~e (1977); Diptera by Hopkins (1966), Gupta and Berridge (1966) and Berridge and Gupta (1967); and Hymenoptera by Davies and King (1975). However, apart from a description of the crytonephridial system in the meal worm Tenebrio molitor L. by Grimstone et al (1968), little is known regarding the structure in other Coleoptera.…”

Section: Introductionmentioning

confidence: 99%

A comparison of rectal pad structure in some terrestrial and intertidal Carabid beetles (Insecta)

Elliott

King

1985

Journal of Natural History

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The structure and ultrastructure of the rectal pads, in two fully terrestrial, and three intertidal species of carabid, were examined. The ultrastructure of the rectal pads is compatible with the theory that they are involved in osmotic regulation. Differences in ultrastructure, particularly the number and structure of the mitochondria, indicate that the intertidal species may be better adapted for osmoregulation in a salt-rich environment. One species, CiIIenus Iaterate (Samouelle) lives near the top of the intertidal zone and its rectal pads display intermediate structural features.

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The fine‐structural changes observed in the rectal papillae of the mosquito Aedes aegypti, L. and their relation to the epithelial transport of water and inorganic ions

Cited by 59 publications

References 25 publications

P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquitoAedes aegypti

P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquitoAedes aegypti

Ultrastructural organization of the rectal pads in adultStenophylax permistusMcL. (Trichoptera)

A comparison of rectal pad structure in some terrestrial and intertidal Carabid beetles (Insecta)

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