The Fimbrial and Non-Fimbrial Haemagglutinins of Escherichia Coli

Haemagglutination and siderophore production as the urovirulence markers of uropathogenic<i> <i>Escherichia</i> coli</i>

“…This test was carried out as per the direct bacterial haemagglutination test -slide method and mannosesensitive and mannose-resistant haemagglutination tests. [5] The strains of E. coli were inoculated into 1% nutrient broth and incubated at 37 °C for 48 hours for full Þ mbriation. A panel of red blood cells was selected by obtaining blood from guinea-pig, sheep and human (blood group 'O').…”

The role of different K antigens of Escherichia coli in phagocytosis by polymorphonuclear leukocytes

“…The first mechanism for interference of K antigens with phagocytosis that we studied was the effect on common pili. Common pili adhere to red blood cells and PMNL via specific receptor interaction (Bar-Shavit et al, 1977;Duguid et al, 1979;Mangan and Snyder, 1979;Silverblatt et al, 1979). Mangan and Snyder (1979) using a bacteria/PMNL ratio of 300/1, showed that the interaction of bacteria with PMNL via pili in the absence of serum resulted in CL production.…”

“…For phagocytosis, the cells were harvested by centrifugation and washed in phosphate-buffered saline (PBS) pH 7-4. For haemagglutination experiments the bacteria were grown in nutrient broth for 24-48 h to improve pili production (Duguid, Clegg and Wilson, 1979). Broth supplemented with glucose 0.050/, was used to enhance the production of K antigens (Orskov et al, 1971).…”

“…We tested the MSHA properties of K + and K-cells. Broth cultures incubated for 24 and 48 h were used to enhance pili production (Duguid et al, 1979). The difference in haemagglutination titre was a factor of two at the most (table 11).…”

Hemagglutinating Factor (HAF) Associated with Adhesiveness in Enteroinvasive Escherichia coli (EIEC)

“…The expression of adhesins by several enteropathogenic E. coli strains has been associated with bacterial colonization of epithelial surfaces and the initiation of infection (6,7,9,24). Bacteria possessing adhesins on their surface often cause hemagglutination (HA) of erythrocytes, which contain the appropriate receptor.…”