The fight for invincibility: Environmental stress response mechanisms and Aeromonas hydrophila

Awan, Furqan; Dong, Yuhao; Wang, Nannan; Jin, Longcun; Ma, Ke; Liu, Yongjie

doi:10.1016/j.micpath.2018.01.023

Cited by 77 publications

(44 citation statements)

References 144 publications

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“…Formation of biofilms in the marine environment assist in the survival, adaptation, and propagation of bacteria (Awan et al, 2018). In some cases, formation is enhanced after introduction of sub-lethal stress or nutritional-stress factors in the environment.…”

Section: Spill Impacts On Biofilm Taxonomymentioning

confidence: 99%

“…Hamdan et al (2018) demonstrated that residual spill contaminants remained at U-166 at the time BMPs were deployed and recovered, and that impacts to sediment microbiomes were evident at moderately and heavily impacted sites. The presence of residual oil may promote bacterial growth and enhance biofilm formation, indicating a community response under adverse environmental conditions (Dombrowski et al, 2016;Awan et al, 2018). Previous studies have shown Alteromonadales to be early and dominant colonizers of marine biofilms (Arora and Boon, 2012;Dombrowski et al, 2016;Rampadarath et al, 2017), with some Alteromonas spp.…”

Section: Spill Impacts On Biofilm Taxonomymentioning

confidence: 99%

“…Many bacteria use the widespread two-component system (TCS) to detect and respond to changes in the environment (Arora and Boon, 2012;Awan et al, 2018). The TCS is deployed as a defense and stress response mechanism, where the cell detects stimuli and responds through selected processes (e.g., quorum sensing, light detection, and chemotaxis).…”

Section: Spill Impacts On Functional Potentialmentioning

confidence: 99%

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Deep-Sea Biofilms, Historic Shipwreck Preservation and the Deepwater Horizon Spill

et al. 2019

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Exposure to oil from the Deepwater Horizon spill may have lasting impacts on preservation of historic shipwrecks in the Gulf of Mexico. Submerged steel structures, including shipwrecks, serve as artificial reefs and become hotspots of biodiversity in the deep sea. Marine biofilms on submerged structures support settlement of microand macro-biota and may enhance and protect against corrosion. Disruptions in the local environment, including oil spills, may impact the role that biofilms play in reef preservation. To determine how the Deepwater Horizon spill potentially impacted shipwreck biofilms and the functional roles of the biofilm microbiome, experiments containing carbon steels disks (CSDs) were placed at five historic shipwreck sites located within, and external to the benthic footprint of the Deepwater Horizon spill. The CSDs were incubated for 16 weeks to enable colonization by biofilm-forming microorganisms and to provide time for in situ corrosion to occur. Biofilms from the CSDs, as well as sediment and water microbiomes, were collected and analyzed by 16S rRNA amplicon gene sequencing to describe community composition and determine the source of taxa colonizing biofilms. Biofilm metagenomes were sequenced to compare differential gene abundances at spill-impacted and reference sites. Biofilms were dominated by Zeta-, Alpha-, Epsilon-, and Gamma-proteobacteria. Sequences affiliated with the Mariprofundus and Sulfurimonas genera were prolific, and Roseobacter, and Colwellia genera were also abundant. Analysis of 16S rRNA sequences from sediment, water, and biofilms revealed sediment to be the main known source of taxa to biofilms at impacted sites. Differential gene abundance analysis revealed the two-component response regulator CreC, a gene involved in environmental stress response, to be elevated at reference sites compared to impacted sites within the spill plume fallout area on the seafloor. Genes for chemotaxis, motility, and alcohol dehydrogenases were differentially abundant at reference vs. impacted sites. Metal loss on CSDs was elevated at sites within the spill fallout plume. Time series images reveal that metal loss at a heavily impacted site, the German Submarine U-166, has accelerated since the spill in 2010. This study provides evidence that spill residues on the seafloor may impact biofilm communities and the preservation of historic steel shipwrecks.

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Section: Spill Impacts On Biofilm Taxonomymentioning

confidence: 99%

Section: Spill Impacts On Biofilm Taxonomymentioning

confidence: 99%

Section: Spill Impacts On Functional Potentialmentioning

confidence: 99%

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Deep-Sea Biofilms, Historic Shipwreck Preservation and the Deepwater Horizon Spill

et al. 2019

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“…Additionally, environmental conditions, including changes in temperature, acidic pH and starvation, may affect its virulence, leading to the production of harmful cytotoxic products (Awan et al . ). A. hydrophila is generally considered a major secondary pathogen that causes disease in fish and amphibians but it can also cause gastroenteritis in humans.…”

Section: Introductionmentioning

confidence: 97%

“…Its distinct characteristics include survivability and reproduction in diverse environments because it remains viable across a wide temperature range (from 4°C to 40°C). Additionally, environmental conditions, including changes in temperature, acidic pH and starvation, may affect its virulence, leading to the production of harmful cytotoxic products (Awan et al 2018). A. hydrophila is generally considered a major secondary pathogen that causes disease in fish and amphibians but it can also cause gastroenteritis in humans.…”

Section: Introductionmentioning

confidence: 99%

Three species ofAeromonas(A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia

Guo

Yang

et al. 2019

Lett Appl Microbiol

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Hundreds of farmed Siamese crocodiles (Crocodylus siamensis) died during July 2016 at a farm in Wenchang, Hainan, China. In two necropsied crocodiles, we observed symptoms of dermatorrhagia, hepatomegaly and hepatic congestion. Pulmonitis was diagnosed by pulmonary congestion and pulmonary fibrinous exudate. Septicaemia was diagnosed by isolation of three Aeromonas species from blood and visceral tissues; A. dhakensis, A. hydrophila and A. jandaei were identified by biochemical and molecular tests. We used a zebrafish model to determine the half‐maximal lethal dose (LD50), and A. dhakensis was found to be the most virulent species, with an LD50 of 8·91 × 105 CFU per ml. The results of a drug sensitivity test indicated that these species were sensitive to 11 antibiotics. This is the first report of A. dhakensis, A. hydrophila and A. jandaei being isolated from a mixed infection in Siamese crocodiles. Significance and Impact of the Study In this study, we isolated three species of Aeromonas (A. dhakensis, A. hydrophila and A. jandae) from farmed Siamese crocodiles with fatal fibrinous pneumonia and septicaemia. This is the first description of a mixed infection with three Aeromonas species among captive crocodilians.

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Genomic and Transcriptomic Diversification of Flagellin Genes Provides Insight into Environmental Adaptation and Phylogeographic Characteristics in Aeromonas hydrophila

Roh,

Kannimuthu

2024

Microb Ecol

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Aeromonas hydrophila is an opportunistic motile pathogen with a broad host range, infecting both terrestrial and aquatic animals. Environmental and geographical conditions exert selective pressure on both geno- and phenotypes of pathogens. Flagellin, directly exposed to external environments and containing important immunogenic epitopes, may display significant variability in response to external conditions. In this study, we conducted a comparative analysis of ~ 150 A. hydrophila genomes, leading to the identification of six subunits of the flagellin gene (fla-1 to fla-4, flaA, and flaB). Individual strains harbored different composition of flagellin subunits and copies. The composition of subunits showed distinct patterns depending on environmental sources. Strains from aquatic environments were mainly comprised of fla-1 to fla-4 subunits, while terrestrial strains predominated in groups harboring flaA and flaB subunits. Each flagellin showed varying levels of expression, with flaA and flaB demonstrating significantly higher expression compared to others. One of the chemotaxis pathways that control flagellin movement through a two-component system was significantly upregulated in flaA(+ 1)/flaB(+ 1) group, whereas flaA and flaB showed different transcriptomic expressions. The genes positively correlated with flaA expression were relevant to biofilm formation and bacterial chemotaxis, but flaB showed a negative correlation with the genes in ABC transporters and quorum sensing pathway. However, the expression patterns of fla-2 to fla-4 were identical. This suggests various types of flagellin subunits may have different biological functions. The composition and expression levels of flagellin subunits could provide valuable insights into the adaptation of A. hydrophila and the differences among strains in response to various external environments.

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The fight for invincibility: Environmental stress response mechanisms and Aeromonas hydrophila

Cited by 77 publications

References 144 publications

Deep-Sea Biofilms, Historic Shipwreck Preservation and the Deepwater Horizon Spill

Deep-Sea Biofilms, Historic Shipwreck Preservation and the Deepwater Horizon Spill

Three species ofAeromonas(A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia

Genomic and Transcriptomic Diversification of Flagellin Genes Provides Insight into Environmental Adaptation and Phylogeographic Characteristics in Aeromonas hydrophila

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