The Fertilizing Capacity of Fowl Semen as Affected by Time and Temperature of Storage

Prolonged Survival of Cock Spermatozoa In Vitro with Fluid Removed from Tissue Cultured Oviducal Cells

“…The number of spermatozoa inseminated was approximately one half of that used by Ashizawa et al (1976). It is generally acknowledged that cock spermatozoa lose their fertilizing ability within 7 hr when preserved in vitro at temperatures above 30 C (Garren and Shaffner, 1952;Schindler et al, 195 5;and Hunsaker et al, 1956). Fertility of cock spermatozoa stored in vitro at 41 C is reported to be only 7 and 3% after storage for 2 or 4 hr, respectively (Schindler et al, 1955).…”

Evidence for Absence of an Effect of Temperature Shock on Fertilizing Ability of Fowl Spermatozoa

“…Lake (1960) took precautions against tempera¬ ture shock by pre-heating the glass funnel to 35°C and holding it in the palm of the hand during collection. Similarly, Hunsaker, Aitken & Lindblad (1956) received semen into glassware warmed to a temperature of 30°C. Glassware at room temperature was used by Garren & Shaffner (1952) and by Polge (1955).…”

Changes in the pH of Semen of the Domestic Cock as Affected by Temperature and Frequency of Collection

“…Parker et al (1942) varied collection frequencies from 6 hours to 8 days, and found a similar tendency. Temperature might also be a variable contributing to the change of pH of cock semen, since it has been shown to have a marked effect on fertilizing capacity (Garren and Shaffner, 1952;Schindler et al, 1955;Hunsaker et al, 1956).…”