The Fermentative Production of L-Citrulline

Okumura, Susumu; Shibuya, Masao; Konishi, Shimpachi; ISHIDA, Moriyoshi; Shiro, Teruo

doi:10.1271/bbb1961.28.742

Cited by 6 publications

(3 citation statements)

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“…This pathway is regulated by the levels of arginine present in a wide variety of bacteria [1,2,3,4,5]. Arginine-requiring mutants produce large amounts of citruline or ornithine or when arginine is limiting [6,7] although their wild-type strains donot accumulate arginine due to these feedback controls by arginine. Arginine produced by mutants with a defective regulatory mechanism and that regulatory mutants could be found among the group of analogue-resistant mutants.…”

Section: Introductionmentioning

confidence: 99%

Production of L-Arginine from arginine analogue resistant mutants of Corynebacterium glutamicum

Musini¹,

Mangamoori²,

Saida³

2018

IJSRBS

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L-arginine is an essential amino acid, widely used in pharmaceutical, feed and food industry. It is the precursor molecule in citrulline and nitric oxide synthesis, which in turn plays significant role in a wide variety of patho -physiological functions in the cardiovascular, central and peripheral nervous systems. In the present study, mutagenesis experiments were carried out for isolation of analogue resistant mutants against Corynebacterium glutamicum. Initially, MIC (Minimum inhibitory concentration) was identified for arginine analogues by tube dilution method. MIC values found to be Arginine hydroximate -680 µg/ml. Further, mutagenesis of C. glutamicum was carried out with NTG (300µg/ml) and amino acid analogue resistant mutants were isolated in minimal media. Fermentation was carried out by resistant colonies and estimated the Arginine levels. Production level was found to be 5.2 mg /ml where as in control cells it was 1.4 mg /ml. This study highlighted the C. glutamicum mutant of L-arginine amino acid production.

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Section: Introductionmentioning

confidence: 99%

Production of L-Arginine from arginine analogue resistant mutants of Corynebacterium glutamicum

Musini¹,

Mangamoori²,

Saida³

2018

IJSRBS

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“…However, the L-arginine level in the broth was not siufficient for industrial production of arginine. On the other hand, many reports in which arginine-requiring mutants were used have been presented on the fermentative production of citrulline (15,18,22). However, the accumulating ability ofL-citrulline was unstable and insufficient for industrialization since the mutants were not genetically released from metabolic control of arginine biosynthesis.…”

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confidence: 99%

Increase in arginine and citrulline production by 6-azauracil-resistant mutants of Bacillus subtilis

Kisumi¹,

Takagi²,

Chibata³

1977

Appl Environ Microbiol

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“…This pathway has been shown to be regulated by arginine in a wide variety of bacteria (2, 11,19,20,22). Arginine-requiring mutants produce large amounts of ornithine or citrulline when arginine is limiting (7,12), although their wild-type strains do not accumulate arginine due to these feedback controls by arginine. We considered that arginine would be produced by mutants with a defective regulatory mechanism of arginine, and that such regulatory mutants could be found among a group of analogue-resistant mutants.…”

mentioning

confidence: 99%

Production of l -Arginine by Arginine Hydroxamate-Resistant Mutants of Bacillus subtilis

1971

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l -Arginine hydroxamate inhibited the growth of various bacteria, and the inhibition was readily reversed by arginine. l -Arginine hydroxamate (10 −3 m ) completely inhibited the growth of Bacillus subtilis . This inhibitory effect was prevented by 2.5 × 10 −4 m l -arginine, which was the most effective of all the natural amino acids in reversing the inhibition. l -Arginine hydroxamate-resistant mutants of Bacillus subtilis were isolated and found to excrete l -arginine in relatively high yields. One of the mutants, strain AHr-5, produced 4.5 mg of l -arginine per ml in shaken culture in 3 days.

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The Fermentative Production of L-Citrulline

Cited by 6 publications

References 0 publications

Production of L-Arginine from arginine analogue resistant mutants of Corynebacterium glutamicum

Production of L-Arginine from arginine analogue resistant mutants of Corynebacterium glutamicum

Increase in arginine and citrulline production by 6-azauracil-resistant mutants of Bacillus subtilis

Production of l -Arginine by Arginine Hydroxamate-Resistant Mutants of Bacillus subtilis

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