The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis

Preez, J. C. du; Bosch, M.; Prior, Bernard A.

doi:10.1007/bf00261920

Cited by 173 publications

(75 citation statements)

References 23 publications

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“…Similar results were reported for P. stipitis and other pentose-fermenting yeasts, C. shehatae and P. tannophilus (Du Preez et al, 1986;Neirinck et al, 1982). However, in the present study, the mutants appeared to utilize slightly more arabinose than the WT in both defined media and diluted HW SSL.…”

Section: Discussionsupporting

confidence: 88%

Mutants of the pentose‐fermenting yeast Pichia stipitis with improved tolerance to inhibitors in hardwood spent sulfite liquor

Bajwa

Shireen

D'Aoust

et al. 2009

Biotech & Bioengineering

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Mutants of Pichia stipitis NRRL Y-7124 able to tolerate and produce ethanol from hardwood spent sulfite liquor (HW SSL) were obtained by UV mutagenesis. P. stipitis cells were subjected to three successive rounds of UV mutagenesis, each followed by screening first on HW SSL gradient plates and then in diluted liquid HW SSL. Six third generation mutants with greater tolerance to HW SSL as compared to the wild type (WT) were isolated. The WT strain could not grow in HW SSL unless it was diluted to 65% (v/v). In contrast, the third generation mutants were able to grow in HW SSL diluted to 75% (v/v). Mutants PS301 and PS302 survived even in 80% (v/v) HW SSL, although there was no increase in cell number. All the third generation mutants exhibited higher growth rates but significantly lower growth yields on xylose or glucose compared to the WT. The mutants fermented 4% (w/v) glucose as efficiently as the WT and fermented 4% (w/v) xylose more efficiently with a higher ethanol yield than the WT. In a medium containing 4% (w/v) each of xylose and glucose, all the third generation mutants utilized glucose as efficiently and xylose more efficiently than the WT. This resulted in higher ethanol yield by the mutants. The mutants retained the ability to utilize galactose and mannose and ferment them to ethanol. Arabinose was consumed slowly by both the mutants and WT with no ethanol production. In 60% (v/v) HW SSL, the mutants utilized and fermented glucose, mannose, galactose and xylose while the WT could not ferment any of these sugars.

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Section: Discussionsupporting

confidence: 88%

Mutants of the pentose‐fermenting yeast Pichia stipitis with improved tolerance to inhibitors in hardwood spent sulfite liquor

Bajwa

Shireen

D'Aoust

et al. 2009

Biotech & Bioengineering

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“…1 shows assimilation pathways of glucose, xylose, and cellobiose in native yeasts. Most yeasts such as Saccharomyces cerevisiae can consume only C 6 sugars (9), while a few other yeasts such as Pichia stipitis (also known as Scheffersomyces stipitis) can assimilate both C 6 and C 5 sugars (10). To grow on xylose as a sole carbon source, yeasts need xylose-specific transporters to import xylose into the cytosol followed by conversion of xylose to xylitol mediated by a xylose reductase (XYL1), conversion of xylitol to xylulose mediated by a xylulose dehydrogenase (XYL2), and conversion of xylulose to xylulose-5-phospate mediated by a xylulose kinase (XYL3) and finally to other precursor metabolites of core metabolism required for cell growth (11).…”

mentioning

confidence: 99%

Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica

Ryu

Hipp

Trinh

2016

Appl Environ Microbiol

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The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism of Y. lipolytica for using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and elucidated the native sugar metabolism in Y. lipolytica for cell growth on xylose and cellobiose as well as their mixtures with glucose through comprehensive metabolic and transcriptomic analyses. We identified 7 putative glucose-specific transporters, 16 putative xylose-specific transporters, and 4 putative cellobiose-specific transporters that are transcriptionally upregulated for growth on respective single sugars. Y. lipolytica is capable of using xylose as a carbon source, but xylose dehydrogenase is the key bottleneck of xylose assimilation and is transcriptionally repressed by glucose. Y. lipolytica has a set of 5 extracellular and 6 intracellular ␤-glucosidases and is capable of assimilating cellobiose via extra-and intracellular mechanisms, the latter being dominant for growth on cellobiose as a sole carbon source. Strikingly, Y. lipolytica exhibited enhanced sugar utilization for growth in mixed sugars, with strong carbon catabolite activation for growth on the mixture of xylose and cellobiose and with mild carbon catabolite repression of glucose on xylose and cellobiose. The results of this study shed light on fundamental understanding of the complex native sugar metabolism of Y. lipolytica and will help guide inverse metabolic engineering of Y. lipolytica for enhanced conversion of biomass-derived fermentable sugars to chemicals and fuels.

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“…Pichia stipitis is one of the better xylose-fermenting yeasts [30,31]. However, the rate of fermentation of glucose by the yeast is lower than that of the common glucose-fermenting microorganisms such as S. cerevisiae and Z. mobilis [32].…”

Section: Effect Of Oxygen Supply On Ethanol Production From Glucose Omentioning

confidence: 99%

Clarification of Interactions among Microorganisms and Development of Co-culture System for Production of Useful Substances

Taniguchi

Tanaka

2004

Recent Progress of Biochemical and Biomedical Engineering in Japan I

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The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis

Cited by 173 publications

References 23 publications

Mutants of the pentose‐fermenting yeast Pichia stipitis with improved tolerance to inhibitors in hardwood spent sulfite liquor

Mutants of the pentose‐fermenting yeast Pichia stipitis with improved tolerance to inhibitors in hardwood spent sulfite liquor

Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica

Clarification of Interactions among Microorganisms and Development of Co-culture System for Production of Useful Substances

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