The female- and male-produced vitellogenins of Leucophaea maderae

Don-Wheeler, Grace; Engelmann, Franz

doi:10.1016/0022-1910(91)90002-h

Cited by 19 publications

(10 citation statements)

References 26 publications

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“…In L. maderae also, Vg synthesis is strictly controlled by JH or its analogues. Vg induction by JH and/or its analogues has been demonstrated even in males and nymphs, although in traces, of some cockroach species including N. cinerea , D. punctata , B. germanica and L. maderae (Don‐Wheeler & Engelmann and references therein). In L. maderae , a JH analogue, methoprene was used for Vg induction in adult females and males.…”

Section: Hormonal Regulation Of Vg Gene Transcription In Different Inmentioning

confidence: 99%

Regulation of vitellogenin genes in insects

Tufail

Nagaba

Elgendy

et al. 2014

Entomological Science

135

127

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Vitellogenins (Vg) genes code for the major egg yolk protein precursor in insects and many other oviparous species. In insects, the Vg gene is expressed extra-ovarially in the fat body in sex-, tissue-and stage-specific manners. During the reproductive phase, the Vg mRNA is expressed in large quantities, which is then translated, secreted into hemolymph and ultimately taken up by the developing oocytes through receptormediated endocytosis. Once sequestered, the Vgs are stored as vitellin (Vn), the main nutritional reserve for the developing embryo. The regulation of Vg genes is directly under the control of hormones at the transcriptional level. Hormones involved in Vg gene transcription are juvenile hormone (JH), ecdysteroids and some neuropeptides. The overall understanding that has emerged is that the insects can be classified, based on the system of hormonal regulation of Vg gene transcription, into three groups: (i) insects (like most of hemipterans) that use only JH for Vg gene transcription; (ii) insects (like dipterans) that need both JH and ecdysteroids for Vg regulation; and (iii) insects like lepidopterans that require JH, ecdysteroids and additional hormones to regulate their reproductive biology. However, why insect species diverge in using different hormones to govern their reproductive physiology remains unclear. The present contribution focuses on the current status of knowledge regarding the regulation of Vg genes in insects. Besides a brief information on biochemical and molecular features, the role of Vg genes as a target of endocrine disruptors will be addressed. Also, the molecular mechanism of Vg gene regulation will be discussed.

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Section: Hormonal Regulation Of Vg Gene Transcription In Different Inmentioning

confidence: 99%

Regulation of vitellogenin genes in insects

Tufail

Nagaba

Elgendy

et al. 2014

Entomological Science

135

127

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“…The anti-LmVg1 peptide antiserum reacted strongly but only to 87-kD Vg/ Vn polypeptides. Don-Wheeler and Engelmann (1991Engelmann ( , 1997 reported, based on immunological techniques and using a short radiopulse with 35 Smethionine/cysteine and 32 P-orthophosphate, that 112-kD L. maderae Vg polypeptide is further cleaved Fig. 2.…”

Section: Immunoblotting and Post-transcriptional Processing Of L Madmentioning

confidence: 99%

“…into an 85-87-kD polypeptide. Also they reported that the 85-87-kD band is barely visible on SDS-PAGE (Don-Wheeler and Engelmann, 1991Engelmann, , 1997. The immunological detection of ~87-kD Vg/Vn polypeptides by both antisera in our study is consistent with results reported by Don-Wheeler and Engelmann (1991Engelmann ( , 1997, and thus confirm LmVg to be a product of two precursor polypeptides.…”

Section: Immunoblotting and Post-transcriptional Processing Of L Madmentioning

confidence: 99%

“…Also they reported that the 85-87-kD band is barely visible on SDS-PAGE (Don-Wheeler and Engelmann, 1991Engelmann, , 1997. The immunological detection of ~87-kD Vg/Vn polypeptides by both antisera in our study is consistent with results reported by Don-Wheeler and Engelmann (1991Engelmann ( , 1997, and thus confirm LmVg to be a product of two precursor polypeptides. The present immunoblot results suggest that (1) Vg1-112-kD polypeptide is completely cleaved into 87 kD as no antigen is detected at the 112-kD position, and that the cleavage site should be at the Cterminus of the 112-kD polypeptide.…”

Section: Immunoblotting and Post-transcriptional Processing Of L Madmentioning

confidence: 99%

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Evidence for two vitellogenin‐related genes in Leucophaea maderae: The protein primary structure and its processing

Tufail

Bembenek

Elgendy

et al. 2007

Arch Insect Biochem Physiol

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We previously reported a cDNA for vitellogenin (Vg) from the cockroach, Leucophaea maderae (Lm). In the present study, we identified another cDNA encoding a second Vg (Vg2) having stretches of amino acid sequences different from the first one, Vg1, reported earlier. The complete nucleotide sequence of Vg2 consisted of 5,915 bp, which encoded a primary protein of 1,911 residues including a 16-residue putative signal peptide. The regions different in both Vg precursors (Pro-Vg1 and pro-Vg2) were four in number, and two, relatively longer, existed at the carboxy terminal. The presence of two Vg-related cDNAs was confirmed by sequencing of RT-PCR products generated using primers designed based on the common sequences flanking the regions different in amino acid sequences. Both forms were transcribed since they could be amplified on mRNA from fat bodies of different individual females. Southern blot analysis of digested genomic DNA revealed the existence of two Vg-related genes in L. maderae indicating that each Vg cDNA originated from a separate gene. Also, the immunoblot analysis using antibodies generated against peptides unique to both Vg1 and Vg2 probed the same antigen in the same individual, suggesting LmVg to be a product coded by two different Vg precursors. Both Vg primary products showed 96% similarity at an amino acid level. Compared to other insect Vgs, Vg2 showed a slightly higher (1-2%) similarity than Vg1. We previously reported, based on amino-terminal sequence analysis, that L. maderae pro-Vg was cleaved into four subunit polypeptides (112-, 100-, 92-, and 55-kD), which were deposited in the egg as four respective vitellin (Vn) polypeptides. We show now based on immunoblot analysis that the 112-kD polypeptide is further cleaved, near the C-terminus, to an 87-kD polypeptide before it is secreted into the hemolymph. Both the L. maderae Vgs were compared with each other and with other insect Vgs and the processing pattern is discussed.

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“…Vitellogenin biosynthesis in Neobellieria, Lucilia, Phormia, and Drosophila males is stimulated by 20-OH-ecdysone De Loof, 1977, 1981]. In the cockroach Leucophaea, on the other hand, JH induces vitellogenin biosynthesis in males [Don-Wheeler and Engelmann, 1991]. In Aedes, both JH and 20-OH-ecdysone are needed for vitellogenesis [Borovsky et al, 1985].…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis and regulation of juvenile hormone III, juvenile hormone III bisepoxide, and methyl farnesoate during the reproductive cycle of the grey fleshfly,Neobellieria (Sarcophaga) bullata

Bylemans

Borovsky

Ujvary

et al. 1998

Arch. Insect Biochem. Physiol.

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To study the effect of brain signals on the biosynthesis of juvenile hormone by the corpora allata of the grey fleshfly Neobellieria bullata, exposed corpora allata connected to the brain were surgically removed from sugar‐fed flies and incubated in vitro with L‐[3H‐methyl]methionine. After incubation, the media together with the tissues were analyzed by HPLC. [3H]Juvenile hormone III (JH III), [3H]JH III bisepoxide (BE), [3H]methyl farnesoate (MF) and an unknown [3H]labeled metabolite (Un) were identified as the primary products. The rate of synthesis of [3H]JH III bisepoxide was higher than that of [3H]JH III, [3H]MF and [3H]Un. Two days after a liver meal, female flies synthesized more JH III, MF, BE, and the Un than did males. Synthesis of JH III, BE, and MF in females was lower during the previtellogenic, sugar‐feeding period than during the vitellogenic liver‐feeding period. Isolated corpus cardiacum–corpus allatum (CC‐CA) complexes that were incubated in vitro synthesized less JH III, MF, and BE, as compared to complexes that were attached to the brain, indicating that the brain probably modulates the biosynthesis of JH III, MF, and BE in the corpora allata. Upon incubation of brain–CC–CA complexes with Neb‐TMOF (10–8 M), Neb‐colloostatin (10–8 M), ovarian, or brain extracts resulted in significant inhibition of JH III and BE biosynthesis in the presence of ovarian extracts. These results indicate that allatostatin‐like factors are present in the ovary of the flesh fly. Arch. Insect Biochem. Physiol. 37:248–256, 1998. © 1998 Wiley–Liss, Inc.

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The female- and male-produced vitellogenins of Leucophaea maderae

Cited by 19 publications

References 26 publications

Regulation of vitellogenin genes in insects

Regulation of vitellogenin genes in insects

Evidence for two vitellogenin‐related genes in Leucophaea maderae: The protein primary structure and its processing

Biosynthesis and regulation of juvenile hormone III, juvenile hormone III bisepoxide, and methyl farnesoate during the reproductive cycle of the grey fleshfly,Neobellieria (Sarcophaga) bullata

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