The features of microbiota isolated from the honeycombs with affected bee brood

Abstract

Aim. To investigate the microbiota of honeycombs with affected bee brood. Methods. Visual, immunochro- matographic, cultural-morphological, biochemical, electron-microscopic methods were used to isolate and previously identify a number of microorganisms. Results. 10 samples of honeycombs with sealed brood were studied. The following agents of bee diseases were isolated and previously identifi ed: Paenibacillus larvae – American foulbrood; Вasillus paraalvei – parafoulbrood; Melissococcus pluton – European foulbrood. Con- clusions. The number of cocci, spore-forming coli and yeasts, found in the samples of honeycombs, commonly represent normal microfl ora of bees, but their number increases signifi cantly in case of viral and bacterial infections.

Antibacterial effect of flower extracts on microorganisms isolated from honeycombs with affected bee brood

“…The objects of our study were 10 strains of bacteria, isolated from 10 samples of honeycombs with sealed bee brood, selected in May-August of 2012-2014 in Kyiv and Zhytomyr regions [9]. The cultures were kept at the temperature of +4 º in the medium of meat-andpeptone agar (MPA) under the layer of paraf nic oil.…”