The feature of migration and deoxygenation of radionuclides U(Ⅵ) on genetically recombined bacteria W1

Abstract

Hoping resolve uranium release caused many harmful environmental impacts by genetic engineering bacteria, which is considered desirable and an innovative environmental-friendly water remediation strategy. Here, we report the great U deoxygenation potential of E. coli. DH5α W1 which was introduced the Dsr A gene of sulfate-reducing bacteria to strengthen its deoxygenation performance for deoxygenating U (VI) to U (IV) and reducing it’s toxic. In static adsorption, the bacteria will undergo a release phenomenon immediately after a super-adsorption stage, but the process of U(VI) deoxygenation was not affected. Through TEM, XPS and FTIR, infer feature of U(Ⅵ) how to migration and deoxygenation in the cell. The cells can reach up to 23.65 mmol of U per g of bacterial wet biomass through active biosorption to enrich U, at 90min, the U (IV) content in the bacteria can account for as high as 94% of the bio-immobilized U (VI). Results presented in this work will help to understand bioaccumulation characteristics better of strong deoxygenation bacteria and create an effective material to remove U.