The Feasibility of Antioxidants Avoiding Oxidative Damages from Reactive Oxygen Species in Cryopreservation

Liu, Xiangjian; Xu, Yiming; Liu, Fenglin; Pan, Yuxin; Liu, Miao; Zhu, Qubo; Tan, Songwen

doi:10.3389/fchem.2021.648684

Cited by 35 publications

(27 citation statements)

References 52 publications

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“…However, we found that 52% and 14% of cells located in the more differentiated areas B and C, respectively, showed intact cellular structure after cryotherapy, in contrast to the surviving cells distributed as small islands in the meristem and young leaf primordia of cryotreated shoot tips of banana (Helliot et al, 2002) and garlic (Vieira et al, 2015). This is consistent with the proposal of Allium species (Block, 2010;Shahrajabian et al, 2020), because antioxidants alleviate oxidative damage from reactive oxygen species produced in cryopreservation (Liu et al, 2021). Therefore, as is demonstrated in the Figure 5e, the low virus-free frequency after cryotherapy might be the result of the distribution of virus close to the meristem apex and the high level of cell survival observed in cryotreated shoot tips.…”

Section: Discussionsupporting

confidence: 89%

Double‐edged effects of the cryogenic technique for virus eradication and preservation in shallot shoot tips

Hamborg

Blystad

et al. 2021

Plant Pathology

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Shallot (Allium cepa var. aggregatum) is a small-bulb onion crop mainly grown in subtropical to temperate regions of the world (Block, 2010;Fritsch & Friesen, 2002). It is consumed for its unique flavour, pungency, and health-enhancing properties (Block, 2010;Fritsch & Friesen, 2002;Shahrajabian et al., 2020). Shallots are predominantly propagated by bulbs, resulting in the accumulation and dissemination of viruses in commercially grown fields and germplasm collections (Katis et al., 2012;Smékalová et al., 2017).The main viruses prevailing in Allium crops are from the genera Potyvirus, Carlavirus, and Allexivirus, which form viral complexes (Katis et al., 2012). Virus infections, particularly by potyviruses like onion yellow dwarf virus (OYDV) and leek yellow stripe virus

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Section: Discussionsupporting

confidence: 89%

Double‐edged effects of the cryogenic technique for virus eradication and preservation in shallot shoot tips

Hamborg

Blystad

et al. 2021

Plant Pathology

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“…Gamete cryopreservation leads to several cellular and molecular alterations (reviewed in detail by Estudillo et al [17]). Among them, the most important to highlight are the damage to the cellular and intracellular membranes [18] and ROS formation [19]. As a consequence of very slow freezing rates, an efflux of intracellular water occurs, leading to an intracellular hyperosmotic environment that prevents the formation of ice [20,21].…”

Section: Cryoinjurymentioning

confidence: 99%

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

Ribeiro

Carrageta

Bernardino

et al. 2022

Animals

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Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function. The correct cryopreservation procedure is characterized by the balance between freezing rate and cryoprotective agents (CPAs), which minimizes cellular dehydration and intracellular ice formation. For this purpose, osmoregulation is a central process in cryopreservation. During cryopreservation, water and small solutes, including penetrating cryoprotective agents, cross the plasma membrane. Aquaporins (AQPs) constitute a family of channel proteins responsible for the transport of water, small solutes, and certain gases across biological membranes. Thirteen homologs of AQPs (AQP0-12) have been described. AQPs are widely distributed throughout the male and female reproductive systems, including the sperm and oocyte membrane. The composition of the male and female gamete membrane is of special interest for assisted reproductive techniques (ART), including cryopreservation. In this review, we detail the mechanisms involved in gamete cryopreservation, including the most used techniques and CPAs. In addition, the expression and function of AQPs in the male and female gametes are explored, highlighting the potential protective role of AQPs against damage induced during cryopreservation.

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“…During cryopreservation, cells may suffer many damages, such as osmotic dehydration, large ice puncture and oxidative damages from reactive oxygen species (ROS). Classical cryoprotectants are employed to reduce osmotic and mechanical damages, but fail to dispose ROS (Liu et al 2021). Furthermore, cryoprotectants used to support cell resistance to cryoinjury can cause oxidative stress at high concentrations (Ren et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of dynamics of global DNA methylation during the cryopreservation process of Pinus pinaster embryogenic lines

Mendoza-Poudereux

Cano

Solís

et al. 2022

Preprint

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Long-term in vitro maintenance of embryogenic lines of Pinus species has been associated with lower maturation capacity, because of this, cryopreservation protocols for the embryogenic lines are needed to maintain valuable genotypes. Since cryopreservation may induce epigenetic variations, we evaluate changes in DNA methylation levels through the course of the cryopreservation of maritime pine embryogenic lines, as compared to those lines maintained by repeated subcultures. Six maritime pine embryogenic lines were cryopreserved following a protocol that includes pre-treatments inducing osmotic stress in liquid media. The percentage of methylated cytosines (%5-mC) in total DNA was determined by using a colorimetric assay. Reactive oxygen species (ROS) accumulation in cell lines was also determined by quantifying dihydroethidium (DHE) fluorescence under a confocal laser-scanning microscope. In the first experiment, we found that global DNA methylation was significantly reduced during the cryopreservation protocol. Subsequently, we evaluated the methylation status of both cryopreserved and no cryopreserved lines (maintained by subcultures) and found differences among embryogenic lines but overall, cryopreservation did not alter %5-mC of the recovered lines while periodical subcultures increased methylation rates. In addition, maltose pretreatment did not increase significantly ROS production in embryogenic lines. Our results demonstrate that the genetic stability during cryopreservation highly depends on the embryogenic line studied, but the protocol allows maintaining methylation DNA rates in most of the recovered lines.

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The Feasibility of Antioxidants Avoiding Oxidative Damages from Reactive Oxygen Species in Cryopreservation

Cited by 35 publications

References 52 publications

Double‐edged effects of the cryogenic technique for virus eradication and preservation in shallot shoot tips

Double‐edged effects of the cryogenic technique for virus eradication and preservation in shallot shoot tips

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

Assessment of dynamics of global DNA methylation during the cryopreservation process of Pinus pinaster embryogenic lines

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