The FcεRI signaling pathway is involved in the pathogenesis of lacrimal gland benign lymphoepithelial lesions as shown by transcriptomic analysis

Abstract

This study aimed to analyze the role of the FcepsilonRI (FcεRI) signaling pathway in the pathogenesis of benign lymphoepithelial lesion of lacrimal gland (LGBLEL). Transcriptomic analysis was performed on LGBLEL and orbital cavernous hemangioma (CH) patients diagnosed via histopathology in Beijing Tongren Hospital, Capital Medical University, between July 2010 and October 2013. Four LGBLEL and three orbital CH patients, diagnosed between October 2018 and August 2019, were randomly selected as experimental and control groups, respectively. RT-PCR, immunohistochemical staining, and western blotting were used to verify genes and proteins related to the FcεRI signaling pathway. Transcriptomic analysis showed that the FcεRI signaling pathway was upregulated in the LGBLEL compared with the CH group. The mRNA expression levels of important genes including SYK, p38, JNK, PI3K, and ERK were significantly increased in the LGBLEL group (P = 0.0066, P = 0.0002, P = 0.0003, P < 0.0001, P < 0.0001, respectively). Immunohistochemical staining results showed that SYK, p38, and ERK were positively expressed in LGBLEL, while JNK and PI3K were not. The protein contents of P-SYK, P-p38, P-JNK, P-PI3K, and P-ERK were significantly higher in the LGBLEL than in the CH group (P = 0.0169, P = 0.0074, P = 0.0046, P = 0.0157, P = 0.0156, respectively). The FcεRI signaling pathway participates in the pathogenesis of LGBLEL.

Evaluation of the Efficacy of Immune and Inflammatory Markers in the Diagnosis of Lacrimal-Gland Benign Lymphoepithelial Lesion

“…Lacrimal-gland benign lymphoepithelial lesion is an immune-related inflammatory lesion whose pathogenesis might be related to the FcepsilonRI, receptor (FcεRI), B cells, T cells, and complement system signaling pathways [ 3 , 9 , 10 ]. With economic and societal developments, LGBLEL has become a more common orbital inflammatory disease.…”

“…Biotin-labeled secondary antibody (ab205718, Abcam) was added after 5 min, and the tissue sections were incubated at 37 °C for 30 min. The tissue was washed three more times with PBS, DAB stained, rinsed with water, hematoxylin stained, and mounted [ 3 ]. Hematoxylin served as a counterstain, and images were captured with an automatic spectroscopic imaging platform (Vectra Ⅱ, PerkinElmer, USA).…”

“…Benign lymphoepithelial lesion, also known as Mikulicz disease, occurs in the parotid gland, lacrimal gland, and other sites and is characterized by lymphocyte infiltration associated with epithelial hyperplasia [ 1 , 2 ]. Lacrimal-gland benign lymphoepithelial lesion (LGBLEL) is an immune-related inflammatory lesion that is most common in middle-aged women [ 3 , 4 ]. The main manifestations are diffuse enlargement of the bilateral or unilateral lacrimal glands and eyelid swelling.…”

“…The main manifestations are diffuse enlargement of the bilateral or unilateral lacrimal glands and eyelid swelling. Typical pathological manifestations are diffuse infiltration of lymphocytes and plasma cells in the lacrimal tissue, atrophy and disappearance of glands, and hyperplasia of the fibrous tissue [ 3 , 4 ]. Studies have shown elevated immunoglobulin 4 (IgG4) expression levels in some LGBLEL serum and tissues; therefore, LGBLEL with positive IgG4 expression is considered IgG4-related ophthalmic disease (IgG4-ROD) [ 5 ].…”

Ubiquitin-related lncRNAs: The new tool for prognosis prediction in prostate cancer

“…Existing studies do not provide specific evidence for the roles of the FC epsilon RI signaling pathway in the pathogenesis of Pca. However, the FC epsilon RI signaling pathway has been shown to participate in the regulation of multiple downstream signaling pathways such as the PI3K-AKT (31) and MAPK pathways (32), which have been shown to be strongly associated with the pathogenesis of Pca (33)(34)(35). As is well known, the FC epsilon RI signaling pathway is activated by immunoglobulin E (IgE) (36).…”