2004
DOI: 10.1021/ac0415511
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Cited by 5 publications
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“…After derivatization, peptide concentrations were determined using the bicinchoninic acid (BCA) assay 19. An angiotensin II standard curve was constructed and used to determine the total peptide concentration in the methylated and non‐methylated peptide samples.…”
Section: Methodsmentioning
confidence: 99%
“…After derivatization, peptide concentrations were determined using the bicinchoninic acid (BCA) assay 19. An angiotensin II standard curve was constructed and used to determine the total peptide concentration in the methylated and non‐methylated peptide samples.…”
Section: Methodsmentioning
confidence: 99%
“…An opaque band at the interface between the 5% and 30% layers was routinely harvested in fraction 4 and contained the lipid rafts. 125 I‐containing fractions were counted in a gamma counter, and aliquots were analyzed by the bicinchoninic acid method for total protein [31], and by immunoblotting for specific proteins resolved by electrophoresis on sodium dodecyl sulfate (SDS)–polyacrylamide gels.…”
Section: Methodsmentioning
confidence: 99%
“…The final pellet was resuspended in buffer B (0.25 M sucrose, 10 mM EDTA, 1 mM PMSF, 250 μM vitamin E, and 1 mM dithiothreitol, pH 7.5) and stored at −80°C for further analysis. The microsomal protein concentrations were determined using the bicinchoninic acid procedure [27].…”
Section: Methodsmentioning
confidence: 99%