The Fatty Acid-Based Erythrocyte Membrane Lipidome in Dogs with Chronic Enteropathy

Crisi, Paolo Emidio; Luciani, A; Tommaso, Morena Di; Prasinou, Paraskevi; Santis, Francesca De; Chatgilialoglu, Chryssostomos; Pietra, Marco; Procoli, Fabio; Sansone, Anna; Giordano, Maria Veronica; Gramenzi, Alessandro; Ferreri, Carla; Boari, Andrea

doi:10.3390/ani11092604

Cited by 9 publications

(18 citation statements)

References 81 publications

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“…The effects of LR on these metabolites was not examined. Furthermore, only a few studies have investigated the lipidomics profile of canine RBC or serum of animals in the course of different diseases 60,80,[85][86][87][88] or undergoing different dietary regimens. 89 Non Leukoreduced units were characterized by higher concentrations of arginine catabolites, especially creatinine, a marker of amino acid catabolism that is used as a proxy for renal dysfunction when measured in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Effect of leukoreduction on the omics phenotypes of canine packed red blood cells during refrigerated storage

Miglio,

Rocconi,

Cremoni

et al. 2024

Veterinary Internal Medicne

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BackgroundRed blood cell (RBC) storage promotes biochemical and morphological alterations, collectively referred to as storage lesions (SLs). Studies in humans have identified leukoreduction (LR) as a critical processing step that mitigates SLs. To date no study has evaluated the impact of LR on metabolic SLs in canine blood units using omics technologies.ObjectiveCompare the lipid and metabolic profiles of canine packed RBC (pRBC) units as a function of LR in fresh and stored refrigerated (up to 42 days) units.AnimalsPacked RBC units were obtained from 8 donor dogs enrolled at 2 different Italian veterinary blood banks.Study Design and MethodsObservational study. A volume of 450 mL of whole blood was collected using Citrate‐Phosphate‐Dextrose‐Saline‐Adenine‐Glucose‐Mannitol (CPD‐SAGM) transfusion bags with a LR filter to produce 2 pRBC units for each donor, without (nLR‐pRBC) and with (LR‐pRBC) LR. Units were stored in the blood bank at 4 ± 2°C. Sterile weekly samples were obtained from each unit for omics analyses.ResultsA significant effect of LR on fresh and stored RBC metabolic phenotypes was observed. The nLR‐pRBC were characterized by higher concentrations of free short and medium‐chain fatty acids, carboxylic acids (pyruvate, lactate), and amino acids (arginine, cystine). The LR‐pRBC had higher concentrations of glycolytic metabolites, high energy phosphate compounds (adenosine triphosphate [ATP]), and antioxidant metabolites (pentose phosphate, total glutathione).Conclusion and Clinical ImportanceLeukoreduction decreases the metabolic SLs of canine pRBC by preserving energy metabolism and preventing oxidative lesions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of leukoreduction on the omics phenotypes of canine packed red blood cells during refrigerated storage

Miglio,

Rocconi,

Cremoni

et al. 2024

Veterinary Internal Medicne

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“…Then, the composition of the lipid bilayer strongly affects the metabolic homeostasis of the cell, the permeability and rheological properties of the membrane, and the functionality of proteins included in it [125, 126]. In 2021, Crisi et al [127] compared the previously described results on healthy subjects [125] with the lipidomic profile of RBCs from dogs with chronic enteropathies, finding significant differences. In particular, they reported decreased levels of linoleic and palmitic acid in contrast to increased levels of dihomo-γ-linolenic acid methyl ester and stearic acids, reflecting abnormal metabolic pathways as observed in human enteropathies such as Crohn’s disease [128] and coeliac disease [129].…”

Section: Integrative Multiomics Approaches In Veterinary Medicinementioning

confidence: 99%

Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine

Miglio,

Cremonini,

Leonardi

et al. 2023

Transfus Med Hemother

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Background: Omics technologies represent a new analytical approach that allows a full cellular readout through the simultaneous analysis of thousands of molecules. The application of such technologies represents a flourishing field of research in human medicine, especially in transfusion medicine, while their application in veterinary medicine still needs to be developed. Summary: Omics technologies, especially proteomics, metabolomics, and lipidomics, are currently applied in several fields of human medicine. In transfusion medicine, the creation and integration of multiomics datasets have uncovered intricate molecular pathways occurring within blood bags during storage. In particular, the research has been directed toward the study of storage lesions (SLs), i.e., those biochemical and structural changes that red blood cells (RBCs) undergo during hypothermic storage, their causes, and the development of new strategies to prevent them. However, due to their challenges to perform and high costs, these technologies are hardly accessible to veterinary research, where their application dates back only to the last few years and thus a great deal of progress still needs to be made. As regards veterinary medicine, there are only a few studies that have focused mainly on fields such as oncology, nutrition, cardiology, and nephrology. Other studies have suggested omics datasets that provide important insights for future comparative investigations between human and nonhuman species. Regarding the study of storage lesions and, more generally, the veterinary transfusion field, there is a marked lack of available omics data and results with relevance for clinical practice. Key Messages: The use of omics technologies in human medicine is well established and has led to promising results in blood transfusion and related practices knowledge. Transfusion practice is a burgeoning field in veterinary medicine, but, to date, there are no species-specific procedures and techniques for the collection and storage of blood units and those validated in the human species are univocally pursued. Multiomics analysis of the species-specific RBCs’ biological characteristics could provide promising results both from a comparative perspective, by increasing our understanding of species suitable to be used as animal models, and in a strictly veterinary view, by contributing to the development of animal-targeted procedures.

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“…These studies uncovered variations in the metabolism of equine RBC during a storage period of up to 35 days of both whole blood (WB) 1,12 and erythrocyte concentrate 32 . Understanding the timecourse and the extent of the equine pRBC storage lesions is critical for the development of optimized storage strategies for this species 29‐31,33‐35 …”

Section: Introductionmentioning

confidence: 99%

“…32 Understanding the timecourse and the extent of the equine pRBC storage lesions is critical for the development of optimized storage strategies for this species. [29][30][31][33][34][35] The aim of the present study was to evaluate lipidomic and metabolomics profiles of equine fresh and stored leukoreduced pRBC (LR-pRBC) and non-leukoreduced pRBC (nLR-pRBC) to characterize metabolic profiles and their changes related to the development, extent, and progression of storage lesions in stored equine blood.…”

mentioning

confidence: 99%

Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage

Miglio,

Rocconi,

Cremonini

et al. 2024

Veterinary Internal Medicne

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BackgroundUnderstanding of the biochemical and morphological lesions associated with storage of equine blood is limited.ObjectiveTo demonstrate the temporal sequences of lipid and metabolic profiles of equine fresh and stored (up to 42 days) and leukoreduced packed red blood cells (LR‐pRBC) and non‐leukoreduced packed RBC (nLR‐pRBC).AnimalsPacked RBC units were obtained from 6 healthy blood donor horses enrolled in 2 blood banks.MethodsObservational study. Whole blood was collected from each donor using transfusion bags with a LR filter. Leukoreduction pRBC and nLR‐pRBC units were obtained and stored at 4°C for up 42 days. Sterile weekly sampling was performed from each unit for analyses.ResultsRed blood cells and supernatants progressively accumulated lactate products while high‐energy phosphate compounds (adenosine triphosphate and 2,3‐Diphosphoglycerate) declined. Hypoxanthine, xanthine, and free fatty acids accumulated in stored RBC and supernatants. These lesions were exacerbated in non‐LR‐pRBC.Conclusion and Clinical ImportanceLeukoreduction has a beneficial effect on RBC energy and redox metabolism of equine pRBC and the onset and severity of the metabolic storage lesions RBC.

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The Fatty Acid-Based Erythrocyte Membrane Lipidome in Dogs with Chronic Enteropathy

Cited by 9 publications

References 81 publications

Effect of leukoreduction on the omics phenotypes of canine packed red blood cells during refrigerated storage

Effect of leukoreduction on the omics phenotypes of canine packed red blood cells during refrigerated storage

Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine

Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage

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