The Fathers of Italian Histology

Abstract

Subscriptions, cancellations, business correspondence and any enquiries must be sent to the Tipografia PIME Editrice srl, Pavia, Italy. Cancellations must be received before the end of September to take effect at the end of the same year.No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means (electronic, electrostatic, magnetic type, mechanical, photocopying or otherwise) without written permission by the Publishers.Reg. Tribunale di Pavia n. 289/23.2.1984. Supported by the Ministero per i Beni e le Attività Culturali, Italy as a publication of high cultural value.Associato all'USPI Unione Stampa Periodica Italiana Disclaimer. Whilst every effort is made by the publishers and the editorial board to see that no inaccurate or misleading data, opinion or statement appears in this journal, they wish to make it clear that the data and opinions appearing in the articles or advertisements herein are the responsibility of the contributor or advisor concerned. Accordingly, the publisher, the editorial board and their respective employees, officers and agents accept no liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement. The Journal publishes original papers, technical reports, letters to the editor, review articles concerning investigations performed with the aid of biophysical, biochemical, molecular-biological, enzymatic, immunohistochemical, cytometric, and image analysis techniques. Areas of particular interest to the European Journal of Histochemistry include: -functional cell and tissue biology in animals and plants; -cell differentiation and death; -cell-cell interaction and molecular trafficking; -biology of cell development and senescence; -nerve and muscle cell biology; -cellular basis of diseases During this period, Carlo Rizzoli established the experimental approach for the study of the molecular basis of cell differentiation in vitro, anticipating some aspects of the present investigation on the potentiality of stem cells. Furthermore, Carlo Rizzoli was one of the first Italian scientists to publish its scientific reports in large-diffusion international journals, thus contributing to the world-wide diffusion of the seminal studies on the in vitro cell differentiation models. Editor-in-ChiefIn 1961, Carlo Rizzoli became Professor of Histology and general embryology and, since 1964 to 1999, Director of the Institute of Histology at the University of Bologna.The initial steps of this undertaking were challenging, since in 1963, following the recruitment of Oliviero Mario Olivo at the Chair of Human Anatomy, the facilities of the Institute of Histology were almost nonexistent. In few years, however, Carlo Rizzoli was able to organize an efficient research group of motivated young collaborators that included Paolo Carinci, Lia Guidotti, Francesco Antonio Manzoli, capable of introducing original and seminal lines of research into the national and international histological arena. In this way, a numb...

The Need for a Consensus on the Locution “Central Nuclei” in Striated Muscle Myopathies

“…Anecdotally speaking, it seems that “centrally located nuclei” is favored by non-English speaker groups, mostly scattered through Europe or East Asia, even though “central nuclei” remains prevalent (Musarò et al, 2007; Coletti et al, 2013; Ikutomo et al, 2014). …”

Muscle acellular scaffold as a biomaterial: effects on C2C12 cell differentiation and interaction with the murine host environment

“…The following couples of primers were used for murine (m) or human (h) genes: (1) glyceraldehyde 3-phosphate dehydrogenase (mGAPDH: forward CTGAGCAAGAGAGGCCCTA; reverse TATGGGGGTCTGGGATGGAA; (2) runt-related transcription factor 2 (mRUNX2: forward TTGACCTTTGTCCCAATGC; reverse AGGTTGGAGGCACACATAGG); (3) alkaline phosphatase (mALP: forward TGTCTGGAACCGCACTGAACT; reverse CAGTCAGGTTGTTCCGATTCAA); (4) glyceraldehyde 3-phosphate dehydrogenase (GAPDH: forward CCAGCAAGAGCACAAGAGGA; reverse AGATTCAGTGTGGTGGGGG); (5) runt-related transcription factor 2 (hRUNX2: forward GAATCCTCCACCCACCCAAG; reverse AATGCTGGGTGGCCTACAAA); (6) bone sialoprotein 2 (hIBSP: forward CCATTCTGGCTTTGCATCCG; reverse GACAAGAAGCCTATTACTTTGC); (7) bone sialoprotein 2 (hOC: forward GTGCAGAGTCCAGCAAAGGT; reverse TCCCAGCCATTGATACAGGT); (8) alkaline phosphatase (hALP: forward GTGCAGAGTCCAGCAAAGGT; reverse TCCCAGCCATTGATACAGGT); (9) myogenin (forward GCACTGGAGTTCGGTCCCAA; reverse TATCCTCCACCGTGATGCTG); (10) Myo D (forward ACCCAGGAACTGGGATATGGA; reverse AAGTCGTCTGCTGTCTCAAA); (11) embryonic myosin heavy chain (e-MHC: forward CGTCTGCTTTTGGCAA; reverse TGGTCGTAATCAGCAGCA). RUNX2 and ALP represent classic markers of osteogenic differentiation (Coquelin et al, 2012; Leotot et al, 2013), while MyoD, myogenin and myosin represent the standard markers for myogenic differentiation (De Arcangelis et al, 2003, 2005; Naro et al, 2003; Musaro et al, 2007). …”