The fate of X-ray-induced chromosome aberrations in blood lymphocyte culture

Das, B. C.; Sharma, T.

doi:10.1016/0027-5107(87)90256-9

Cited by 14 publications

(2 citation statements)

References 24 publications

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“…It is assumed, especially for theoretical work, that all paired fragments retain their adherence, and are transmitted to MN as a unit pair (Braselmann et al, 1986;Carrano and Heddle, 1973;Wakata and Sasaki, 1987). Recent evidence from certain species indicates that this may not always be the case (Das and Sharma, 1987), so that in some systems, two MN may sometimes arise from one AF pair. c) Fragment coalescence.…”

Section: Fragment-fate Factorsmentioning

confidence: 99%

Micronuclei: pitfalls and problems

Savage¹

2011

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Section: Fragment-fate Factorsmentioning

confidence: 99%

Micronuclei: pitfalls and problems

Savage¹

2011

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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“…et al, 1983a,b;Poncelet et al, 1988 , and decline of Ž induced aberrations in ÕiÕo Bender and Gooch, 1963;Norman et al, 1966;Sasaki and Norman, 1967;Schmid and Bauchinger, 1969;Carrano and Heddle, 1973;Brasel-. mann et al, 1986;Das and Sharma, 1987 , as major biological sources of variability of the aberration rate.…”

Section: ( ) Deõelopment and Standardization Of The Dicentric Dic Assaymentioning

confidence: 99%

How radiation-specific is the dicentric assay?

Hoffmann

Schmitz-Feuerhake

1999

J Expo Sci Environ Epidemiol

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Question: Quantitative cytogenetic analysis of structural chromosome aberrations in human peripheral blood lymphocytes is widely used as an assay to Ž . detect and quantify exposure to a variety of clastogens. Unstable chromosome rearrangements, dicentric chromosomes dic and centric ring chromosomes Ž . Ž . centric r , are routinely used as biomarkers to assess human exposure to ionizing radiation "biological dosimetry" . In the moderate-to high-dose range many authors consider the dic assay sufficiently radiation-specific for both practical and legal purposes. However, specificity has never been evaluated in quantitative terms. The high sensitivity of the assay would in principle allow for applications in the range of low-dose exposure which has been a major concern in epidemiologic studies. Validity of the assay then critically depends on specificity. Methods: A mathematical model is proposed which includes as parameters the decline of dicentric aberrations in ÕiÕo, the linear dose coefficient for the induction of dic and the average total radiation exposure of the population. To assess radiation-specificity of the dic assay the equilibrium dicentric rate due to radiation is compared to measurements of the background rate of dic in unexposed controls. Results: Environmental and medical radiation combined account for at least about 80% of the average background level of dicentrics. Conclusion: It is concluded that the dicentric assay is highly specific for ionizing radiation and can therefore be used to assess prior exposure in the dose range of interest in environmental epidemiology.

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Rapid metaphase and interphase detection of radiation‐induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization

Cremer¹,

Popp²,

Emmerich³

et al. 1990

Cytometry

131

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Chromosomal in situ suppression (CISS)‐hybridization of biotinylated phage DNA‐library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes in irradiated human peripheral lymphocytes. In addition, probe pUC1.77 was used to mark the Iq12 subregion in normal and aberrant chromosomes 1. Low LET radiation (60Co‐γ‐rays; 1.17 and 1.33 MeV) of lymphocyte cultures was performed with various doses (D = 0, 2, 4, 8 Gy) 5 h after stimulation with phytohaemagglutinin. Irradiated cells were cultivated for an additional 67 h before Colcemid arrested metaphase spreads were obtained. Aberrations of the specifically stained chromosomes, such as deletions, dicentrics, and rings, were readily scored after in situ hybridization with either the 1q12 specific probe or DNA‐library inserts. By the latter approach, translocations of the specifically stained chromosomes could also be reliably assessed. A linear increase of the percentage of specifically stained aberrant chromosomes was observed when plotted as a function of the square of the dose D. A particular advantage of this new approach is provided by the possibility to delineate numerical and structural chromosome aberrations directly in interphase nuclei. These results indicate that cytogenetic monitoring of ionizing radiation may be considerably facilitated by CISS‐hybridization.

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The fate of X-ray-induced chromosome aberrations in blood lymphocyte culture

Cited by 14 publications

References 24 publications

Micronuclei: pitfalls and problems

Micronuclei: pitfalls and problems

How radiation-specific is the dicentric assay?

Rapid metaphase and interphase detection of radiation‐induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization

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