The fate of type 7 adenovirions in lysosomes of HeLa cells

The Interaction between the Fiber Knob Domain and the Cellular Attachment Receptor Determines the Intracellular Trafficking Route of Adenoviruses

“…However, at the same time point, more than 60% of Ad5/35L virions were found within endosomes. This is in agreement with observations made for wild-type subgroup B and C viruses (5,7,18) as well as with studies performed with chimeric Ad5/7 vectors (13,14). The latter studies have shown that specific structures within the Ad7 fiber act as a pH-dependent trigger for membrane lysis, allowing virus to escape into the cytosol.…”

“…Ad5-GFP has an unmodified wild-type Ad5 capsid, whereas the Ad5/35L-GFP vector possesses modified fibers with the knob domain derived from human serotype Ad35 (subgroup B). Ad35 has been shown to interact with a primary attachment receptor(s) different from the Ad5 receptor, CAR, on K562 human erythroleukemia cells (22 (5,7,18). Ad5/35L-GFP vector attached to and internalized into HeLa cells approximately three times more efficiently (1,500 virus particles per cell) than Ad5-GFP vector (550 virus particles per cell) (Fig.…”

Adenovirus Serotype 7 Retention in a Late Endosomal Compartment prior to Cytosol Escape Is Modulated by Fiber Protein

“…This has been characterized for subgroup C serotypes 1, 2 and 5, but this property is clearly not shared universally among members of the Adenoviridae family. In particular, members of subgroup B (serotypes 3 and 7) are noted for their relatively long residence times inside membranous organelles, identified as lysosomes based on the ultrastructural appearance of the organelle or cosedimentation of the viral genome with lysosomal markers (6,37). The present study has focused on a functional characterization of the compartments through which Ad7 traffics using colocalization of virions with compartment markers, compartment pH, and membrane lysis.…”

“…Based on previously published ultrastructural studies suggesting aggregation of Ad7 in an endocytic compartment (6,8) and cosedimentation with lysosomal enzymes (6,37), we hypothesized that the subgroup B virus Ad7 follows the lysosomal trafficking pathway after endocytosis. To follow the intra-and analyzed using a cooled charge-coupled device camera (Princeton Instruments, Trenton, N.J.) and Metamorph imaging software (Universal Imaging, West Chester, Pa.) as previously described (26).…”

Initial Stages in Infection with Animal Viruses

“…Those adenoviruses which are translocated across the plasma membrane commence uncoating as they pass through the membrane, as evidenced by a change from a hexagonal to a spherical profile as a particle crosses the plasma membrane (Brown & Burlingham, 1973) and the sensitivity of its genome to DNase (Ogier et al, 1977). (By contrast papovaviruses remain resistant to DNase until they enter the nucleus.)…”