The fate of Pseudomonas anguilliseptica in artificially infected eels Anguilla japonica.

Abstract

The multiplication and distribution of Pseudomonas anguilliseptica in artificially infected Japanese eels were studied by viable cell count. When the eels were injected intramuscularly with the lethal doses (107, 109/100 g fish weight) of a K 1 h to 12 h post-inoculation. This phase was followed by a static phase, in which the organism persisted at lower concentration or grew slowly, and the duration of this phase depended on the inoculated dosage and ambient water temperature. At the final phase, the organism appeared in rapidly increasing number and rose to high levels (108-10 viable cells/g or ml), which persisted until the death of fish. On the contrary, when inoculated with sublethal dose (105 CFU) of the virulent strain, with lethal dose strain, the organism disappeared completely from the tissues except the muscle of the inoculated site within 1-3 days. The final level of viable cell count in the moribund eels was higher than those of Aeromonas hydrophila or Vibrio anguillarum, and this difference was thought to reflect toxin production.

Phenotypic, serological and genetic characterization of Pseudomonas anguilliseptica strains isolated from cod, Gadus morhua L., in northern Europe

“…All the cod isolates belonged to serotype O1. This result is in agreement with previous studies that proposed that serotypes of P. anguilliseptica are associated with the host origin of the isolates rather than their geographical origin (Nakai, Muroga & Wakabayashi 1981; Nakai, Hanada & Muroga 1985a; Nakai, Kanemori, Nakajima & Muroga 1985b; López‐Romalde 2005).…”

Association of Pseudomonas anguilliseptica infection with ‘winter disease’ in sea bream, Sparus aurata L.

“…Table 1 shows the average monthly water temperatures during the WD outbreaks in both years. From these data, it is evident that WD outbreaks occurred when water temperatures were at or below about 12 °C, which confirms previous reports of the association between cold water temperatures and the isolation of P. anguilliseptica from clinical cases in several fish species ( Muroga & Nakajima 1981; Nakai, Kanemori, Nakajima & Muroga 1985; Wiklund & Bylund 1990; Berthe et al . 1995 ).…”

Dynamics of Vibrio sp. PJ in Organs of Orally Infected Kuruma Prawn, Penaeus japonicus.

“…Tissue distribution of bacterial fish pathogens such as Aeromonas hydrophila (Hatai, 1972), Pseudom onas anguilliseptica (Nakai et al, 1985) and Vibrio anguillarum (Muroga and De La Cruz, 1987) in each major host were studied. Similar studies were also conducted with crustacean pathogens in deca pod crustaceans (Cornick and Stewart, 1968;McKay and Jenkins, 1970;White and Ratcliffe, 1982;Factor and Beekman, 1990).…”