The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis

Mangeney, Marianne; Rousselet, Germain; Taga, Samir; Tursz, Thomas; Wiels, Joëlle

doi:10.1016/0161-5890(95)00004-x

Cited by 27 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed rapid death of CD77 ϩ germinal center B cells in vitro suggests that endogenous ligand molecules interact with Gb3/CD77 to bring about the physiologic selection of immature B cells (12,35). Furthermore, the capability of the B subunit of VT to induce apoptosis of Gb3/CD77 ϩ cells (18) strongly encourages the investigation of Gb3/CD77-associating cytoplasmic molecules (34).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning of Globotriaosylceramide/CD77 Synthase, a Glycosyltransferase That Initiates the Synthesis of Globo Series Glycosphingolipids

Kojima¹,

Fukumoto²,

Okajima³

et al. 2000

Journal of Biological Chemistry

129

120

View full text Add to dashboard Cite

The expression cloning of a cDNA for globotriaosylceramide (Gb3)/CD77 synthase (␣1,4-galactosyltransferase) was achieved using an anti-Gb3 antibody and mouse L cells as a recipient cell line for the transfection. The isolated cDNA clone designated pVTR1 predicted a type II membrane protein with 19 amino acids of cytoplasmic domain, 26 amino acids of transmembrane region, and a catalytic domain with 308 amino acids. Introduction of the cDNA clone into L cells resulted in the neosynthesis of Gb3/CD77, and the extracts of the transfectant cells showed ␣1,4-galactosyltransferase activity only on lactosylceramide and galactosylceramide. In Northern blotting, a 2.3-kilobase mRNA was strongly expressed in heart, kidney, spleen, and placenta and weakly in colon, small intestine, and brain. Transfection of the cDNA into L cells resulted in the constitution of sensitivity to the apoptosis with Shiga-like toxins (verotoxins). Since Gb3/CD77 synthase initiates the synthesis of globo series glycolipids, the isolation of this cDNA will make possible further investigations into the function of its important series of glycolipids.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Cloning of Globotriaosylceramide/CD77 Synthase, a Glycosyltransferase That Initiates the Synthesis of Globo Series Glycosphingolipids

Kojima¹,

Fukumoto²,

Okajima³

et al. 2000

Journal of Biological Chemistry

129

120

View full text Add to dashboard Cite

show abstract

“…Refinements of these techniques, particularly those using CD40 monoclonal antibody or CD40-ligand bound to fibroblasts (82,83), have produced impressive systems for long-term culture of germinal centre B cells, but critical evaluation of these methods shows that they fail to generate cells that have the phenotype of normal memory cells. Cells that have some features of memory cells are only produced after several days in culture (83,84). far longer than the 24 hours required to generate memory ceils from germinal centre B cells in vivo.…”

Section: Selection Of Centrocytes and Tbeir Differentiationmentioning

confidence: 99%

The changing preference of T and B cells for partners as T‐dependent antibody responses develop

MacLennan

Gulbranson-Judge

Toellner

et al. 1997

Immunological Reviews

260

190

View full text Add to dashboard Cite

Recirculating virgin CD4+ T cells spend their life migrating between the T zones of secondary lymphoid tissues where they screen the surface of interdigitating dendritic cells. T-cell priming starts when processed peptides or superantigen associated with class II MHC molecules are recognised. Those primed T cells that remain within the lymphoid tissue move to the outer T zone, where they interact with B cells that have taken up and processed antigen. Cognate interaction between these cells initiates immunoglobulin (Ig) class switch-recombination and proliferation of both B and T cells; much of this growth occurs outside the T zones B cells migrate to follicles, where they form germinal centres, and to extrafollicular sites of B-cell growth, where they differentiate into mainly short-lived plasma cells. T cells do not move to the extrafollicular foci, but to the follicles; there they proliferate and are subsequently involved in the selection of B cells that have mutated their Ig variable-region genes. During primary antibody responses T-cell proliferation in follicles produces many times the peak number of T cells found in that site: a substantial proportion of the CD4+ memory T-cell pool may originate from growth in follicles.

show abstract

“…Evidence for a relationship between globotriaosylceramide expression and apoptosis in B lymphocytes (Mangeney et al, 1995) and Burkitt lymphoma cells (Mangeney et al, 1993) has been provided. More recently, it has been shown that apoptosis induced in HuT78 human lymphoma cells by Fas ligation or short-chain ceramide administration is mediated by GD3 ganglioside synthesis and can be prevented by GluCer synthase inhibition (De Maria et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Apoptosis induced by N-hexanoylsphingosine in CHP-100 cells associates with accumulation of endogenous ceramide and is potentiated by inhibition of glucocerebroside synthesis

1998

View full text Add to dashboard Cite

We report that apoptosis induced by N-hexanoylsphingosine (C 6 -Cer) in CHP-100 human neuroepithelioma cells associates with accumulation of monohexosylsphingolipids produced not only by short-chain ceramide glycosylation but also through glycosylation of a ceramide pool endogenously produced. By high-performance thin layer chromatography on borate silica gel plates, newly formed monohexosylsphingolipids were identified as glucosylceramides (GluCer); however, accumulation of lactosylceramide or higher-order glycosphingolipids was not observed. GluCer accumulation was fully suppressed by D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol; moreover, while this inhibitor had no effect on cell viability when administered alone, it markedly potentiated the apoptotic effect of C 6 -Cer. These results provide evidence that activation of GluCer synthesis is an important mechanism through which CHP-100 cells attempt to escape ceramide-induced apoptosis.

show abstract

The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis

Cited by 27 publications

References 23 publications

Molecular Cloning of Globotriaosylceramide/CD77 Synthase, a Glycosyltransferase That Initiates the Synthesis of Globo Series Glycosphingolipids

Molecular Cloning of Globotriaosylceramide/CD77 Synthase, a Glycosyltransferase That Initiates the Synthesis of Globo Series Glycosphingolipids

The changing preference of T and B cells for partners as T‐dependent antibody responses develop

Apoptosis induced by N-hexanoylsphingosine in CHP-100 cells associates with accumulation of endogenous ceramide and is potentiated by inhibition of glucocerebroside synthesis

Contact Info

Product

Resources

About