The fate of free radicals in a cellulose based hydrogel: Detection by electron paramagnetic resonance spectroscopy

Basumallick, Lipika; Ji, Junyan A.; Naber, Nariman; Wang, Y. John

doi:10.1002/jps.21632

Cited by 14 publications

(5 citation statements)

References 14 publications

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“…The signal (⧫) characterized by hyperfine coupling constant ( a N = 16.2 G, a H(β) = 25 G, and g = 2.0012) and in accordance with that of computer simulation (Figure S10) was assigned to the DMPO adduct of carbon-centered LA· . The signal (black spade suit) characterized by a hyperfine coupling constant ( a N = 15.1 G) was assigned to the DMPO derivative, probably attributed to the presence of trace O 2 .…”

Section: Resultssupporting

confidence: 74%

Biomimetic Aerobic Epoxidation of Alkenes Catalyzed by Cobalt Porphyrin under Ambient Conditions in the Presence of Sunflower Seeds Oil as a Co-Substrate

et al. 2020

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In this work, a mild and sustainable catalytic aerobic epoxidation of alkenes catalyzed by cobalt porphyrin was performed in the presence of sunflower seeds oil. Under ambient conditions, the conversion rate of trans-stilbene reached 99%, and selectivity toward epoxide formation was 88%. The kinetic studies showed that the aerobic epoxidation followed the Michaelis−Menten kinetics. Mass spectroscopy and in situ electron spin resonance indicated that linoleic acid was converted to fatty aldehydes via hydroperoxide intermediates. A plausible mechanism of epoxidation of alkenes was accordingly proposed.

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Section: Resultssupporting

confidence: 74%

Biomimetic Aerobic Epoxidation of Alkenes Catalyzed by Cobalt Porphyrin under Ambient Conditions in the Presence of Sunflower Seeds Oil as a Co-Substrate

et al. 2020

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“…Free radicals are difficult to detect because they are highly reactive with a relatively short half-life. The use of spin traps employing an organic nitrone or nitroso compound are often added to the radical generating system to ensure rapid reaction with any radicals to give a stable and detectable nitroxide radical adducts (25)(26)(27). In the polysorbate investigation study, EPR using the spin trap DMPO detected free radicals in the Fab samples formulated using peroxide-containing PS20.…”

Section: Mechanism and Kinetics Of Trp Oxidation In Fab Drug Product mentioning

confidence: 99%

Site-Specific Tryptophan Oxidation Induced by Autocatalytic Reaction of Polysorbate 20 in Protein Formulation

Lam¹,

Lai²,

Chan³

et al. 2011

Pharm Res

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“…Hydroxyl radicals, generated by decomposition of hydrogen peroxide by ferrous ions (Fenton reaction), were detected by EPR spectroscopy using a DMPO spin trap (24,25). The reaction mixture was prepared in 0.1 M potassium phosphate buffer, pH 7.4, containing 500 μ M DMPO, 100 μ M hydrogen peroxide, and 75 μ M ammonium iron (II) sulphate.…”

Section: Detection Of Hydroxyl Radical By Electron Paramagnetic Resonmentioning

confidence: 99%

Effects of Sulfaphenazole Derivatives on Cardiac Ischemia–Reperfusion Injury: Association of Cytochrome P450 Activity and Infarct Size

et al. 2010

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Abstract. Cardiac ischemia-reperfusion injury is evoked by reactive oxygen species (ROS). We previously reported that sulfaphenazole (SPZ) attenuated cardiac ROS levels and ischemia-reperfusion injury in rats. SPZ has distinct two actions: a) elimination of ROS and b) inhibition of cytochrome P450 (CYP) that is responsible for ROS production. The aim of this study is to determine which action contributes to the attenuation of cardiac ischemia-reperfusion injury using SPZ and its derivatives [acetyl-SPZ (Ac-SPZ) and dichloro-SPZ (2Cl-SPZ)]. Administration of 2Cl-SPZ or SPZ prior to ischemia significantly reduced myocardial infarct size, myocardial lipid peroxides, and ROS levels. In addition, they inhibited rat cardiac CYP activity. However, Ac-SPZ neither reduced infarct size nor inhibited cardiac CYP activity. The three compounds had similar effects on ROS scavenging activity in that they scarcely scavenged hydrogen peroxide and superoxide anions but reduced hydroxyl radicals with the same efficacy. The serum concentration of each compound was almost the same until 24 h after reperfusion. Collectively, our findings indicate that the suppressive effects of SPZ and 2Cl-SPZ on ischemia-reperfusion injury are associated with the reduction of ROS levels, which is primarily due to a decrease in ROS production via inhibition of cardiac CYP, not via ROS scavenging activity.

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The fate of free radicals in a cellulose based hydrogel: Detection by electron paramagnetic resonance spectroscopy

Cited by 14 publications

References 14 publications

Biomimetic Aerobic Epoxidation of Alkenes Catalyzed by Cobalt Porphyrin under Ambient Conditions in the Presence of Sunflower Seeds Oil as a Co-Substrate

Biomimetic Aerobic Epoxidation of Alkenes Catalyzed by Cobalt Porphyrin under Ambient Conditions in the Presence of Sunflower Seeds Oil as a Co-Substrate

Site-Specific Tryptophan Oxidation Induced by Autocatalytic Reaction of Polysorbate 20 in Protein Formulation

Effects of Sulfaphenazole Derivatives on Cardiac Ischemia–Reperfusion Injury: Association of Cytochrome P450 Activity and Infarct Size

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