The Fate of 2,4-Dichlorophenoxyacetic Acid in Bean Seedlings. I. Recovery of 2,4-Dichlorophenoxyacetic Acid and Its Breakdown in the Plant

Hay, J. R.; Thimann, Kenneth V.

doi:10.1104/pp.31.5.382

Cited by 18 publications

(8 citation statements)

References 15 publications

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“…Inactivation of 2,4-D in bean plants was recently followed with the pea-curvature auxin assay by Hay & Thimann (59). When 2,4-D was in troduced at a flap cut in one of the primary leaves of the seedling, most of the 2,4-D which was translocated out into the leaf and beyond became in activated in four days (none got beyond the hypocotyl region), whereas the 2,4-D remaining at the point of application persisted, as if the mechanism for inactivation either did not exist there or had become disrupted.…”

Section: Destruction Of Other Auxinsmentioning

confidence: 99%

Destruction of Auxin

Ray¹

1958

Annu. Rev. Plant. Physiol.

167

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A considerable amount of attention has been given to both the bio chemistry and physiology of auxin destruction in plants since the review of this topic by Gordon (54) in 1954 ; the present review deals largely with the progress in this field since Gordon's review. The greatest amount of reCent work has concerned the oxygen-consuming enzymes which destroy indole-3-acetic acid (IAA), and most of the discussion will necessarily be devoted to this aspect of the subject. An effort will also be made to survey other destruction mechanisms which are known, both for IAA and for other auxins. Finally, the relation between these processes and the physi ology of plants will be considered. CHEMISTRY OF IAA DESTRUCTIONPhotooxidation.-Brauner (17) followed riboflavin-sensitized photooxi dation of IAA simultaneously with (a) a H elianthus curvature test, (b) the Salkowski color reaction, (c) the change in pH, which accompanies decarboxyla tion, and (d) the Hopkins-Cole color reaction for the indole nucleus. He found that the changes in different assays did not run parallel, and interpreted the results as indicating that IAA photodestruction was occurring in three steps, the first converting IAA to a physiologically in active, Salkowski-positive acid, the second involving decarboxylation and loss of Salkowski reactivity, and the third forming a product of weak Hopkins-Cole reactivity. This conclusion might be partly questioned, be cause the bioassay showed evidence of being affected by riboflavin, and by illumination of the solutions, in such a way as to make calibration with pure IAA unreliable. However, other evidence also indicates the existence of II. sequence of reactions in photooxidation.Fischer (40) reported the detection, by chromatography and electro phoresis, of indole-3-carboxaldehyde (hereafter called indolealdehyde ) as a production of both ultraviolet and of riboflavin-sensitized photooxidation of IAA. From products of the latter reaction it was isolated by chromatog raphy and identified chemically. Another substance "E," having an RF between that of IAA and that of indolealdehyde, was also detected with the nitrite reagent (similar to the Salkowski reaction); it was isolated and reported to be identical with indole-3-glycolic acid. Fischer (40) ac-'The survey of literature pertaining to this review was concluded in October, 1957.• The following abbreviations will be used: DPNH, diphosphopyridine nucleo tide (reduced form); IAA, indole-3-acetic acid; IAN, indole-3-acetonitrile; 2,4-D, 2,4-dichlorophenoxyacetic acid. 81 Annu. Rev. Plant. Physiol. 1958.9:81-118. Downloaded from www.annualreviews.org by NORTH CAROLINA STATE UNIVERSITY on 09/25/12. For personal use only. Quick links to online content Further ANNUAL REVIEWS

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Section: Destruction Of Other Auxinsmentioning

confidence: 99%

Destruction of Auxin

Ray¹

1958

Annu. Rev. Plant. Physiol.

167

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“…The amounts recovered were assayed with the split peastem test to yield quantitative data on the amounts of unchanged 2,4-D moving to each part of the plant under various conditions. All figures are the average of at least two trials with the result that in some cases data in the tables may be slightly below the minimum determinable in a single test (7). Particular attention was given to the quantities moving to the base of the shoot and to the roots, since this information is not given by methods which depend on the curvature of the epicotyl, or on the inhibition of the terminal bud of seedlings to which 2,4-D has been applied.…”

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confidence: 99%

“…The method used is described in the preceding paper (7). The plants were seedlings of Phaseolus vulgaris, var.…”

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The Fate of 2,4-Dichlorophenoxyacetic Acid in Bean Seedlings. II. Translocation.

Hay¹,

Thimann²

1956

Plant Physiol.

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“…The susceptibility of the red ltidney bean to these two compounds under field conditions is not Itnown. However, in tests where 25 pg of silvex and of 2,4-D were applied through a flap in tlle, prinlary leaf (method described previously (8) ) the former caused considerably lnore darnage to the stems (Hay, unpublished data).…”

Section: Resultsmentioning

confidence: 99%

“…The amount of auxin extracted from the tissues was measured by the slit-pea test. The extraction, purification, and assay methods used here have been described previously (8). None of the known degradation products of 2,4-D which might be extracted by these procedures possess auxin activity.…”

Section: Methodsmentioning

confidence: 99%

Experiments on the Relative Susceptibility of Weeds to Auxin Herbicides

Hay¹

1962

Can. J. Bot.

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Sections of stems of Sileue c u c~c b a l~~s ,Galeopsis tetrahit, and Pl~aseolr~s zulgaiis were culturcd unclcr sterile contlitions in media containing inorganic salts, sucrose, agar, ancl the I~erbicicles 2-methyl, 4-chlorophenoxyacetic acid, 2,4-clichlorophenosyacetic acid, or 2-(2,4,5-trichlorophenoxy)propionic acid. 'I'he herbicide which gave the most eriective control of a species under field condi-'Manuscript

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The Fate of 2,4-Dichlorophenoxyacetic Acid in Bean Seedlings. I. Recovery of 2,4-Dichlorophenoxyacetic Acid and Its Breakdown in the Plant

Cited by 18 publications

References 15 publications

Destruction of Auxin

Destruction of Auxin

The Fate of 2,4-Dichlorophenoxyacetic Acid in Bean Seedlings. II. Translocation.

Experiments on the Relative Susceptibility of Weeds to Auxin Herbicides

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