The fast track to canonical Wnt signaling in MC3T3‐E1 cells protected by substance P against serum deprivation‐induced apoptosis

Yang, Jianguo; Nie, Jiping; Fu, Su; Liu, Song; Wu, Jianxin; Cui, Liang; Yu, Bin

doi:10.1002/cbin.10676

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…At a later time point (21 days) osteoblasts responded differently to SP which is in accordance to data from Yang et al . who reported that stimulation with 10 −10 M SP prevented MC3T3 osteoblasts against serum deprivation-induced apoptosis 28 . Backman and colleagues also demonstrated positive effects of SP on cell survival 29 .…”

Section: Discussionmentioning

confidence: 99%

Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts

Niedermair

Schirner

Seebröker

et al. 2018

Sci Rep

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Clinical observations suggest neuronal control of bone remodeling. Sensory nerve fibers innervating bone, bone marrow and periosteum signal via neurotransmitters including substance P (SP). In previous studies we observed impaired biomechanical and structural bone parameters in tachykinin (Tac) 1-deficient mice lacking SP. Here, we aim to specify effects of SP on metabolic parameters of bone marrow macrophage (BMM)/osteoclast cultures and osteoblasts isolated from Tac1-deficient and wildtype (WT) mice. We demonstrated endogenous SP production and secretion in WT bone cells. Absence of SP reduced bone resorption rate, as we found reduced numbers of precursor cells (BMM) and multinucleated osteoclasts and measured reduced cathepsin K activity in Tac1−/− BMM/osteoclast cultures. However, this might partly be compensated by reduced apoptosis rate and increased fusion potential of Tac1−/− precursor cells to enlarged “super” osteoclasts. Contrarily, increased ALP enzyme activity and apoptosis rate during early osteoblast differentiation accelerated osteogenesis and cell death in the absence of SP together with reduced ALP activity of Tac1−/− osteoblasts during late osteogenic differentiation resulting in reduced bone formation at later stages. Therefore, we suggest that absence of SP presumably results in a slight reduction of bone resorption rate but concomitantly in a critical reduction of bone formation and mineralization rate.

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Section: Discussionmentioning

confidence: 99%

Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts

Niedermair

Schirner

Seebröker

et al. 2018

Sci Rep

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“…2A, Supplemental Table II). The genes linked to apoptosis were decreased, consistent with a survival role for SP (52). The growth factors within the network underscored the complex mechanism by which SP stimulates hematopoiesis (17).…”

Section: Hematopoietic-associated Genes In Sp-stimulated Stromamentioning

confidence: 67%

Neuroimmune/Hematopoietic Axis with Distinct Regulation by the High-Mobility Group Box 1 in Association with Tachykinin Peptides

Gergues

Bliss

Eljarrah

et al. 2020

The Journal of Immunology

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Hematopoiesis is tightly regulated by the bone marrow (BM) niche. The niche is robust, allowing for the return of hematopoietic homeostasis after insults such as infection. Hematopoiesis is partly regulated by soluble factors, such as neuropeptides, substance P (SP), and neurokinin A (NK-A), which mediate hematopoietic stimulation and inhibition, respectively. SP and NK-A are derived from the Tac1 gene that is alternately spliced into four variants. The hematopoietic effects of SP and NK-A are mostly mediated via BM stroma. Array analyses with 2400 genes indicated distinct changes in SP-stimulated BM stroma. Computational analyses indicated networks of genes with hematopoietic regulation. Included among these networks is the high-mobility group box 1 gene (HMGB1), a nonhistone chromatin-associated protein. Validation studies indicated that NK-A could reverse SP-mediated HMGB1 decrease. Long-term culture-initiating cell assay, with or without NK-A receptor antagonist (NK2), showed a suppressive effect of HMGB1 on hematopoietic progenitors and increase in long-term culture-initiating cell assay cells (primitive hematopoietic cells). These effects occurred partly through NK-A. NSG mice with human hematopoietic system injected with the HMGB1 antagonist glycyrrhizin verified the in vitro effects of HMGB1. Although the effects on myeloid lineage were suppressed, the results suggested a more complex effect on the lymphoid lineage. Clonogenic assay for CFU– granulocyte-monocyte suggested that HMGB1 may be required to prevent hematopoietic stem cell exhaustion to ensure immune homeostasis. In summary, this study showed how HMGB1 is linked to SP and NK-A to protect the most primitive hematopoietic cell and also to maintain immune/hematopoietic homeostasis.

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The fast track to canonical Wnt signaling in MC3T3‐E1 cells protected by substance P against serum deprivation‐induced apoptosis

Cited by 2 publications

References 29 publications

Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts

Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts

Neuroimmune/Hematopoietic Axis with Distinct Regulation by the High-Mobility Group Box 1 in Association with Tachykinin Peptides

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