The Family of Chloride Channel Regulator, Calcium-activated Proteins in the Feline Respiratory Tract: A Comparative Perspective on Airway Diseases in Man and Animal Models

Erickson, Nancy A.; Gruber, Achim D.; Mundhenk, Lars

doi:10.1016/j.jcpa.2019.10.193

Cited by 7 publications

(11 citation statements)

References 51 publications

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“…ISH was performed as reported previously [ 33 ] using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer’s instructions with minor adjustments. Probes for the detection of N gene RNA of SARS-CoV-2 (NCBI database NC_045512.2, nucleotides 28,274 to 9533, assay ID: VPNKRHM) and the mouse housekeeping gene eukaryotic translation elongation factor-1α (EF1a; assay ID: VB1-14428-VT, Affymetrix, Inc., Santa Clara, CA, USA), which shares 95% sequence identity with the Syrian hamster orthologue, were designed.…”

Section: Methodsmentioning

confidence: 99%

Age-Dependent Progression of SARS-CoV-2 Infection in Syrian Hamsters

Osterrieder

Bertzbach

Dietert

et al. 2020

Viruses

212

204

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In late 2019, an outbreak of a severe respiratory disease caused by an emerging coronavirus, SARS-CoV-2, resulted in high morbidity and mortality in infected humans. Complete understanding of COVID-19, the multi-faceted disease caused by SARS-CoV-2, requires suitable small animal models, as does the development and evaluation of vaccines and antivirals. Since age-dependent differences of COVID-19 were identified in humans, we compared the course of SARS-CoV-2 infection in young and aged Syrian hamsters. We show that virus replication in the upper and lower respiratory tract was independent of the age of the animals. However, older hamsters exhibited more pronounced and consistent weight loss. In situ hybridization in the lungs identified viral RNA in bronchial epithelium, alveolar epithelial cells type I and II, and macrophages. Histopathology revealed clear age-dependent differences, with young hamsters launching earlier and stronger immune cell influx than aged hamsters. The latter developed conspicuous alveolar and perivascular edema, indicating vascular leakage. In contrast, we observed rapid lung recovery at day 14 after infection only in young hamsters. We propose that comparative assessment in young versus aged hamsters of SARS-CoV-2 vaccines and treatments may yield valuable information, as this small-animal model appears to mirror age-dependent differences in human patients.

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Section: Methodsmentioning

confidence: 99%

Age-Dependent Progression of SARS-CoV-2 Infection in Syrian Hamsters

Osterrieder

Bertzbach

Dietert

et al. 2020

Viruses

212

204

View full text Add to dashboard Cite

show abstract

“…ISH was performed as reported previously ( Erickson et al, 2020 ; Osterrieder et al, 2020 ) using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific) following the manufacturer’s instructions with minor adjustments. Probes for the detection of the Nucleoprotein (N) gene RNA of SARS-CoV-2 (NCBI database NC_045512.2, nucleotides 28,274 to 9,533, assay ID: VPNKRHM), and the murine housekeeping gene eukaryotic translation elongation factor-1α (EF1a; assay ID: VB1–14428-VT, Affymetrix, Inc.), that shares 95% sequence identity with the Syrian hamster, were designed.…”

Section: Methodsmentioning

confidence: 99%

A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model

Kreye

Reincke

Kornau

et al. 2020

Preprint

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The emergence of SARS-CoV-2 led to pandemic spread of coronavirus disease 2019 (COVID-19), manifesting with respiratory symptoms and multi-organ dysfunction. Detailed characterization of virus-neutralizing antibodies and target epitopes is needed to understand COVID-19 pathophysiology and guide immunization strategies. Among 598 human monoclonal antibodies (mAbs) from ten COVID-19 patients, we identified 40 strongly neutralizing mAbs. The most potent mAb CV07-209 neutralized authentic SARS-CoV-2 with IC50 of 3.1 ng/ml. Crystal structures of two mAbs in complex with the SARS-CoV-2 receptor-binding domain at 2.55 and 2.70 Å revealed a direct block of ACE2 attachment. Interestingly, some of the near-germline SARS-CoV-2 neutralizing mAbs reacted with mammalian self-antigens. Prophylactic and therapeutic application of CV07-209 protected hamsters from SARS-CoV-2 infection, weight loss and lung pathology. Our results show that non-self-reactive virus-neutralizing mAbs elicited during SARS-CoV-2 infection are a promising therapeutic strategy.

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“…ISH was performed as reported previously (Erickson et al, 2020;Osterrieder et al, 2020) VPNKRHM), and the murine housekeeping gene eukaryotic translation elongation factor-1α (EF1a;…”

Section: Histopathology and In Situ Hybridizationmentioning

confidence: 99%

A Sars-Cov-2 Neutralizing Antibody Protects from Lung Pathology in a Covid-19 Hamster Model

et al. 2020

View full text Add to dashboard Cite

The emergence of SARS-CoV-2 led to pandemic spread of coronavirus disease 2019 (COVID-19), manifesting with respiratory symptoms and multi-organ dysfunction. Detailed characterization of virusneutralizing antibodies and target epitopes is needed to understand COVID-19 pathophysiology and guide immunization strategies. Among 598 human monoclonal antibodies (mAbs) from ten COVID-19 patients, we identified 40 strongly neutralizing mAbs. The most potent mAb CV07-209 neutralized authentic SARS-CoV-2 with IC50 of 3.1 ng/ml. Crystal structures of two mAbs in complex with the SARS-CoV-2 receptor-binding domain at 2.55 and 2.70 Å revealed a direct block of ACE2 attachment. Interestingly, some of the near-germline SARS-CoV-2 neutralizing mAbs reacted with mammalian self-antigens. Prophylactic and therapeutic application of CV07-209 protected hamsters from SARS-CoV-2 infection, weight loss and lung pathology. Our results show that non-self-reactive virus-neutralizing mAbs elicited during SARS-CoV-2 infection are a promising therapeutic strategy. .

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The Family of Chloride Channel Regulator, Calcium-activated Proteins in the Feline Respiratory Tract: A Comparative Perspective on Airway Diseases in Man and Animal Models

Cited by 7 publications

References 51 publications

Age-Dependent Progression of SARS-CoV-2 Infection in Syrian Hamsters

Age-Dependent Progression of SARS-CoV-2 Infection in Syrian Hamsters

A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model

A Sars-Cov-2 Neutralizing Antibody Protects from Lung Pathology in a Covid-19 Hamster Model

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