The Family Methanotrichaceae

Oren, Aharon

doi:10.1007/978-3-642-38954-2_277

Cited by 16 publications

(15 citation statements)

References 65 publications

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“…) and the Transect (Supporting Information Fig. S2) consisted at all sites mainly of the (nonmethanogenic) class Thaumarchaeota , except at Igarape where Methanobacteriales, Methanocellales, Methanomicrobiales, Methanosaetaceae [now renamed Methanotrichaceae (Oren, )] , Methanosarcinaceae and Methanomassiliicoccus accounted for > 30% of the total archaeal abundance (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

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Structure, function and resilience to desiccation of methanogenic microbial communities in temporarily inundated soils of the Amazon rainforest (Cunia Reserve, Rondonia)

Hernández

Klose

Claus

et al. 2019

Environmental Microbiology

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Summary The floodplain of the Amazon River is a large source for the greenhouse gas methane, but the soil microbial communities and processes involved are little known. We studied the structure and function of the methanogenic microbial communities in soils across different inundation regimes in the Cunia Reserve, encompassing nonflooded forest soil (dry forest), occasionally flooded Igapo soils (dry Igapo), long time flooded Igapo soils (wet Igapo) and sediments from Igarape streams (Igarape). We also investigated a Transect (four sites) from the water shoreline into the dry forest. The potential and resilience of the CH4 production process were studied in the original soil samples upon anaerobic incubation and again after artificial desiccation and rewetting. Bacterial and archaeal 16S rRNA genes and methanogenic mcrA were always present in the soils, except in dry forest soils where mcrA increased only upon anaerobic incubation. NMDS analysis showed a clear effect of desiccation and rewetting treatments on both bacterial and archaeal communities. However, the effects of the different sites were less pronounced, with the exception of Igarape. After anaerobic incubation, methanogenic taxa became more abundant among the Archaea, while there was only little change among the Bacteria. Contribution of hydrogenotrophic methanogenesis was usually around 40%. After desiccation and rewetting, we found that Firmicutes, Methanocellales and Methanosarcinaceae became the dominant taxa, but rates and pathways of CH4 production stayed similar. Such change was also observed in soils from the Transects. The results indicate that microbial community structures of Amazonian soils will in general be strongly affected by flooding and drainage events, while differences between specific field sites will be comparatively minor.

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Section: Resultsmentioning

confidence: 99%

“…Different letters above the bars indicate significant differences between the treatments. (Oren, 2014)], Methanosarcinaceae and Methanomassiliicoccus accounted for > 30% of the total archaeal abundance ( Fig. 4).…”

Section: Archaeal Community Compositionmentioning

confidence: 99%

Structure, function and resilience to desiccation of methanogenic microbial communities in temporarily inundated soils of the Amazon rainforest (Cunia Reserve, Rondonia)

Hernández

Klose

Claus

et al. 2019

Environmental Microbiology

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“…The family Methanotrichaceae is reported to use only acetate as its substrate [ 40 ]. The hydrogenotrophic methanogens dominated over aceticlastic methanogens ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Dynamic Shift of Microbial Communities during Start-Up, Overloading and Steady-State in an Anaerobic Membrane Bioreactor

Vincent

Wei

Zhang

et al. 2018

IJERPH

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A lab-scale anaerobic membrane bioreactor (AnMBR) with a side stream tubular membrane was developed to treat synthetic domestic sewage to evaluate its performance and the dynamic shift of bacterial and archaeal communities during the start-up, steady-state, overloading and recovery periods of operation at mesophilic temperatures. During the start-up period, the bacterial and archaeal communities changed drastically, and Proteobacteria and Bacteroidetes predominated. During the steady-state period, the AnMBR exhibited excellent COD removal above 91%, and COD of the effluent was below 50 mg/L. High-throughput sequencing analysis results revealed that bacterial and archaeal communities shifted significantly from the start-up to the steady-state period, and that the Proteobacteria phylum predominated on days 140, 162 and 190, and the archaea community hydrogenotrophic methanogen genus Methanolinea (1.5–6.64%) predominated over the aceticlastic methanogen genus Methanothrix (1.35–3.07%). During the overloading period, significant changes occurred in microbial community on day 210, e.g., the phyla Bacteroidetes (30%), Proteobacteria (23%) and Firmicutes (18%) predominated and the archaeal community was completely suppressed, and Methanobrevibacter (0.7%) was the only methanogen genus that emerged in the overloading period. After a shock loading period, the microbial communities exhibited significant changes within the ranks of methanogens and shifted to dominance of the aceticlastic methanogen pathway. In addition, the TVFAs to alkalinity ratio in this study was suitable as an indicator of monitoring performance in the AnMBR operation.

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“…As a further step in understanding the ecology of acetate oxidizers (syntrophic or non-syntrophic ones) versus acetoclastic methanogens, we attempted to document their coexistence by studying lake sediments, which had been reported as containing 16S rRNA genes of putatively acetoclastic Methanosaetaceae (Methanotrichaceae, Oren, 2014) (Ji et al, 2016). We used these sediments and measured the fractions of hydrogenotrophic methanogenesis and of the methyl group of acetate being oxidized to CO 2 rather than reduced to CH 4 and compared the turnover of acetate to the production rate of CH 4 .…”

Section: Introductionmentioning

confidence: 99%

Acetate turnover and methanogenic pathways in Amazonian lake sediments

2020

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Abstract. Lake sediments in Amazonia are a significant source of CH4, a potential greenhouse gas. Previous studies of sediments using 13C analysis found that the contribution of hydrogenotrophic versus acetoclastic methanogenesis to CH4 production was relatively high. Here, we determined the methanogenic pathway in the same sediments (n=6) by applying 14Cbicarbonate or 2-14Cacetate and confirmed the high relative contribution (50 %–80 %) of hydrogenotrophic methanogenesis. The respiratory index (RI) of 2-14Cacetate, which is 14CO2 relative to 14CH4+14CO2, divided the sediments into two categories, i.e., those with an RI < 0.2 consistent with the operation of acetoclastic methanogenesis and those with an RI > 0.4 showing that a large percentage of the acetate-methyl was oxidized to CO2 rather than reduced to CH4. Hence, part of the acetate was probably converted to CO2 plus H2 via syntrophic oxidation, thus enhancing hydrogenotrophic methanogenesis. This happened despite the presence of potentially acetoclastic Methanosaetaceae in all the sediments. Alternatively, acetate may have been oxidized with a constituent of the sediment organic matter (humic acid) serving as oxidant. Indeed, apparent acetate turnover rates were larger than CH4 production rates except in those sediments with a R<0.2. Our study demonstrates that CH4 production in Amazonian lake sediments was not simply caused by a combination of hydrogenotrophic and acetoclastic methanogenesis but probably involved additional acetate turnover.

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The Family Methanotrichaceae

Cited by 16 publications

References 65 publications

Structure, function and resilience to desiccation of methanogenic microbial communities in temporarily inundated soils of the Amazon rainforest (Cunia Reserve, Rondonia)

Structure, function and resilience to desiccation of methanogenic microbial communities in temporarily inundated soils of the Amazon rainforest (Cunia Reserve, Rondonia)

Characterization and Dynamic Shift of Microbial Communities during Start-Up, Overloading and Steady-State in an Anaerobic Membrane Bioreactor

Acetate turnover and methanogenic pathways in Amazonian lake sediments

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