The Fall Armyworm Triose Phosphate Isomerase (<i>Tpi</i>) Gene as a Marker of Strain Identity and Interstrain Mating

Nagoshi, Rod

doi:10.1603/an09046

Cited by 101 publications

(194 citation statements)

References 26 publications

(31 reference statements)

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“…2A). Digestion by MspI produces diagnostic banding patterns of 319 and 639 bp associated with the rice-strain (COI-RS) or 456 and 502 bp for the corn-strain (COI-CS) haplotypes (Nagoshi et al 2007b, Meagher andNagoshi 2010). This method has the advantage of requiring MspI activity to occur to diagnose either strain.…”

Section: Methodsmentioning

confidence: 99%

“…Tpi haplotypes were analyzed by modiÞcations of an earlier method that used 10 single base polymorphisms, distributed over two exons and one intron, as identiÞed by direct DNA sequencing of PCR products (Nagoshi 2010). The original method was often ambiguous for male specimens because these are homogametic (Z/Z) in Lepidoptera and therefore carry two copies of the Tpi gene.…”

Section: Methodsmentioning

confidence: 99%

“…Attempts to Þnd reproducible strain differences under controlled laboratory conditions have had some notable, but limited success. The two strains appear to differ in the timing of important aspects of mating behavior and in the components of the female sex pheromone (Pashley et al 1992, Groot et al 2008, 2010Lima and McNeil 2009;Schoß et al 2009;Groot et al 2010). There are also reports of strain differences in ovipositional substrates, developmental time and viability on various hosts, and mating preferences, though these have often not been in agreement (Pashley and Martin 1987, Whitford et al 1988, Quisenberry 1991, Veenstra et al 1995, Meagher et al 2004.…”

mentioning

confidence: 99%

“…Tpi haplotypes can also distinguish the two strains, and because it is on the Z-chromosome it undergoes a different inheritance pattern from COI. This makes it useful as a complement to the COI method and, in combination with COI, provides a means to identify potential instances of interstrain hybridization (Nagoshi 2010).…”

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confidence: 99%

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Genetic Characterization of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Argentina

Nagoshi¹,

Murúa²,

Hay-Roe³

et al. 2012

jnl. econ. entom.

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Fall armyworm is a major economic pest throughout the Western Hemisphere. Previous studies of populations in the southern United States, Brazil, and the Caribbean demonstrated the existence of two morphologically identical but genetically distinct host strains that can only be distinguished using genetic markers, including polymorphisms in the mitochondrial Cytochrome Oxidase I (COI) gene and in the Z-chromosome linked Triose phosphate isomerase (Tpi) gene. The strains differ in some physiological and behavioral characteristics, most notably their preference for different plant hosts, but are capable of hybridizing in the laboratory and in the field. These traits suggest that the strains are in the process of divergence, which may or may not be hemispheric in scope. The objective of this study was to determine whether the two strains are present in Argentina. It was found that the strain-diagnostic haplotypes of the COI and Tpi genes subdivided the Argentina population into two major groups. Each group displayed biases in their distribution among different host plants that were generally consistent with expected strain behavior. The overall results indicated that Argentina fall armyworm exhibit similar genetics and behavior to populations in the rest of the hemisphere. In addition, the Argentina populations had comparable haplotype frequencies to those from Brazil and Texas, consistent with possible interactions with these fall armyworm groups, but appeared to have had minimal exchanges with those from Puerto Rico or Florida.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genetic Characterization of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Argentina

Nagoshi¹,

Murúa²,

Hay-Roe³

et al. 2012

jnl. econ. entom.

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“…S8). We selected some sf-R and sf-C individuals from each field to genotype according to one SNP on the Tpi gene located on the Z chromosome (Nagoshi 2010) and presence of the FR1…”

Section: And S2e-f)mentioning

confidence: 99%

Transcriptional differences between the two host strains of Spodoptera frugiperda (Lepidoptera: Noctuidae)

Orsucci

Moné

Audiot

et al. 2018

Preprint

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crops such as corn, sorghum and soybean. While FAW larvae are considered polyphagous, differences in diet preference have been described between two genetic variants: the Corn strain (sf-C) and the Rice strain (sf-R). These two strains are sometimes considered as distinct species, raising the hypothesis that host plant specialization might have driven their divergence. Ecological speciation takes place when adaptations to different ecological niches lead to the reproductive isolation of two populations. Under this hypothesis, we expect that the transcriptional response to the host plants should affect differently the fitness of the two FAW strains. We also expect that these genes should also be linked to a reproductive isolation mechanism between the strains. In this study, we performed controlled reciprocal transplant (RT) experiments to address the impact of plant diet on several traits linked to the fitness of the sf-C and sf-R strains. The phenotypical data suggest that sf-C is specialized to corn. We then used RNA-Seq to analyze the gene expression of FAW larvae from RT experiments. We show that each strain has a different response to the same plant diets. However, we also found constitutive transcriptional differences between strains in laboratory and in natural populations. In particular, we show that mitochondrial transcription is the main difference between strains. A difference in mitochondrial function may be the basis for a shift in host plant and could be involved in hybrid incompatibility, raising the hypothesis that mitochondrial genome is the main target of selection between the two strains.. CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. (Goergen et al. 2016;Jeger et al. 2017).The FAW is a polyphagous species, being documented on over 100 plants from 27 different families (Pogue 2002). However, using allozymes electrophoresis monitoring, a significant genetic heterogeneity has been observed in FAW . CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/263186 doi: bioRxiv preprint first posted online Mar. 5, 2018; 4 populations that was associated with feeding preferences (Pashley et al. 1985;Pashley 1986). One genetic haplotype was mostly found on corn (Zea mais), sorghum (Sorghum spp.) and cotton (Gossypium spp.) and was named the corn strain (sf-C). Another haplotype was found associated to individuals collected on smaller grasses such as turf, pasture (Cynodon dactylon) grasses and rice (Oryza spp.), and has been named the rice strain (sf-R) (Pashley 1988). Subsequent studies have confirmed these genetic differences on markers such as the mitochondrial gene Pashley and Martin 1987;Pashley, Hammond, and Hardy 1992). In order to detect post-zygotic...

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Different development and fecundity between Spodoptera frugiperda USA and China populations, influenced by ecdysone‐related genes

Choi,

Mo,

Park

2024

Arch Insect Biochem Physiol

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The fall armyworm (FAW), Spodoptera frugiperda, is one of the most harmful plant pests in the world and is globally distributed from the American continent to the Asian region. The FAW USA population (Sf‐USA) and China population (Sf‐CHN), which belong to corn strain, showed different developmental periods and fecundity rates in lab conditions. Sf‐USA had faster development and higher fecundity compared with Sf‐CHN. To examine these differences, transcriptomic data from two FAW populations were analyzed and compared. Twelve gigabytes of transcripts were read from each sample and 21,258 differentially expressed genes (DEGs) were detected. DEGs with log2 fold change ≥ 2 were identified and compared in two populations. In comparison to the Sf‐CHN, we discovered that 3471 and 3851 individual DEGs upregulated and downregulated, respectively. Comparing transcriptome profiles for differential gene expression revealed several DEGs, including 39 of ecdysone (E)‐, 25 of juvenile hormone‐, and 15 of insulin‐related genes. We selected six of E‐related genes, such as Neverland, Shade, Ecdysone receptor, Ecdysone‐inducible protein 74 (E74), E75, and E78 from DEGs. Gene expressions were suppressed by RNA interference to confirm the physiological functions of the selected genes from Sf‐USA. The Sf‐USA showed developmental retardation and a decrease in fecundity rate by suppression of E‐related genes. These findings show that biological characteristics between Sf‐USA and Sf‐CHN are influenced by E‐related genes.

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The Fall Armyworm Triose Phosphate Isomerase (Tpi) Gene as a Marker of Strain Identity and Interstrain Mating

Cited by 101 publications

References 26 publications

Genetic Characterization of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Argentina

Genetic Characterization of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Argentina

Transcriptional differences between the two host strains of Spodoptera frugiperda (Lepidoptera: Noctuidae)

Different development and fecundity between Spodoptera frugiperda USA and China populations, influenced by ecdysone‐related genes

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