2003
DOI: 10.1046/j.1538-7836.2003.00484.x
|View full text |Cite
|
Sign up to set email alerts
|

The factor VIII assay problem: neither rhyme nor reason

Abstract: To cite this article: Lollar P. The factor VIII assay problem: neither rhyme nor reason. J Thromb Haemost 2003; 1: 2275±9.See also Mor®ni M. A multicenter pharmacokinetic study of the B-domain delted recombinant factor VIII concentrate suing different assays and standards. This issue, pp. 2283±9.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
18
0

Year Published

2004
2004
2017
2017

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 18 publications
(19 citation statements)
references
References 15 publications
1
18
0
Order By: Relevance
“…In the in vitro part of this study, 3 different assays were used for determining FVIII levels, specifically, one-stage and chromogenic FVIII:C assays and an ELISA FVIII antigen assay. We observed the previously described discrepancy in FVIII levels for rFVIII concentrates determined with the one-stage vs. the chromogenic activity assay, with an underestimation with the former assay [23][24][25][26][27]. Levels obtained with the immunological antigen assay were very similar to those obtained with the chromogenic activity assay.…”
Section: Discussionsupporting
confidence: 68%
See 1 more Smart Citation
“…In the in vitro part of this study, 3 different assays were used for determining FVIII levels, specifically, one-stage and chromogenic FVIII:C assays and an ELISA FVIII antigen assay. We observed the previously described discrepancy in FVIII levels for rFVIII concentrates determined with the one-stage vs. the chromogenic activity assay, with an underestimation with the former assay [23][24][25][26][27]. Levels obtained with the immunological antigen assay were very similar to those obtained with the chromogenic activity assay.…”
Section: Discussionsupporting
confidence: 68%
“…Notably, the relative FVIII levels over time were similar with the 3 different assays. For the in vivo study discussed below, we followed the suggestion of Lollar [26], and adopted the one‐stage assay to investigate the stability of diluted KG‐FS during CI.…”
Section: Discussionmentioning
confidence: 99%
“…The inconsistent use of the one‐ or two‐stage assays for the quantification of FVIII has been the source of analytical complication 77. Even within the same assay, use of recombinant or plasma standards can lead to different interpretations of data 54.…”
Section: Resultsmentioning
confidence: 99%
“…In contrast, established fVIII assays have an fVIII time-log linear range of 0.01 to 0.3 units/mL. 44 Thus, a coagulation assay based on the activated platelet membrane rather than PLVs may have potential as the basis of a clinical assay with a broader range. In addition, the sensitivity of the assay to fVIII inhibition by ESH4 and ESH8 differed by .10-fold compared with commercially available 1-stage and chromogenic assays.…”
mentioning
confidence: 99%