The eyestalk–androgenic gland–testis endocrine axis in the crayfish Cherax quadricarinatus

Khalaila, Isam; Manor, Rivka; Weil, Shalva; Granot, Yosef; Keller, Rainer; Sagi, Amir

doi:10.1016/s0016-6480(02)00031-x

Cited by 124 publications

(76 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the protandric shrimp Pandalus platyceros, eyestalk ablation leads to an increase in RNA synthesis in the androgenic gland (AG) and induces spermatogenesis in the testes (Foulks and Hoffman, 1974). After unilateral eyestalk ablation in C. quadricarinatus, the weight of the testes increased at 6 d and decreased after 12 d, which is in accordance with previous findings in this species (Khalaila et al, 2002). Compared with the control, a significant difference in Cq-DDX5 expression was detected 3 d after eyestalk ablation, with a significant increase in the subsequent days resulting in a total sustained expression peaks in response to eyestalk ablation in the first 12 d and a decrease expression at 18 d (Figure 7).…”

Section: Temporal Expression Of Cq-ddx5 After Eyestalk Ablationsupporting

confidence: 91%

A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis

Fang

Wang²,

Wang³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The freshwater crayfish Cherax quadricarinatus, originally from Australia, is an invasive species that is also widely used in aquaculture. DEAD-box helicase family genes are found throughout evolution and encode RNA-binding proteins. The human DDX5 (p68) is important for normal cell growth, differentiation and proliferation. We identified a C. quadricarinatus homolog of DDX5 (Cq-DDX5); the temporal expression of Cq-DDX5 mRNA transcripts was measured during early ontogenesis, during spermatogenesis, during testes development, and during the annual cycle. The Cq-DDX5 cDNA comprises 2258 nucleotides, with an open reading frame of 1569 bp, encoding 522 amino acid residues. The deduced amino acid sequence of Cq-DDX5 has a 53 to 90% similarity to DDX5 of other eukaryotic species. mRNA transcripts of Cq-DDX5 were detected in all tissues, with high levels in the gonads. The DDX5 expression was highest in the nauplii stage, during early ontogenesis and during testes development. In adult testes, transcripts appeared at significantly higher levels in the prespawning and spawning phase than in the post-spawning or regressed phase. Eyestalk ablation resulted in upregulation of Cq-DDX5 in adult ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 3963-3975 (2011) D. male gonads in a time-dependent manner, with a peak at about 12 days. We conclude that the Cq-DDX5 gene plays an important role in early ontogenesis and spermatogenesis, with a crucial reproductive function in germ cell differentiation in these invertebrates.

show abstract

Section: Temporal Expression Of Cq-ddx5 After Eyestalk Ablationsupporting

confidence: 91%

A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis

Fang

Wang²,

Wang³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…A similar experiment might reveal whether such a signal can also induce the persistent changes in thoracic reflexes reported here. If so, the sources of these signals might be sought in the neuroendocrine structures of the eyestalks (Khalaila et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Neural Circuit Reconfiguration by Social Status

Issa¹,

Drummond²,

Cattaert³

et al. 2012

J. Neurosci.

View full text Add to dashboard Cite

The social rank of an animal is distinguished by its behavior relative to others in its community. Although social-status-dependent differences in behavior must arise because of differences in neural function, status-dependent differences in the underlying neural circuitry have only begun to be described. We report that dominant and subordinate crayfish differ in their behavioral orienting response to an unexpected unilateral touch, and that these differences correlate with functional differences in local neural circuits that mediate the responses. The behavioral differences correlate with simultaneously recorded differences in leg depressor muscle EMGs and with differences in the responses of depressor motor neurons recorded in reduced, in vitro preparations from the same animals. The responses of local serotonergic interneurons to unilateral stimuli displayed the same status-dependent differences as the depressor motor neurons. These results indicate that the circuits and their intrinsic serotonergic modulatory components are configured differently according to social status, and that these differences do not depend on a continuous descending signal from higher centers.

show abstract

“…Upstream endocrine regulation of the AG has thus far been described in terms of AG inhibition by the neuroendocrine center located in the eyestalk within the XO-SG (Khalaila et al, 2002). In this respect, extensive AG hypertrophy and hyperplasia upon endocrine manipulation (elimination of this negatively regulating tissue) has previously been documented (Khalaila et al, 2002;Ventura et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Food, comprising shrimp pellets (30% protein) (Rangen Inc., Buhl, ID, USA), was supplied ad libitum three times a week. An endocrine manipulation causing hypertrophy of the AG (hAG) was performed by eyestalk ablation as previously described (Khalaila et al, 2001;Khalaila et al, 2002).…”

Section: Materials and Methods Animalsmentioning

confidence: 99%

An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone

Rosen

Manor

Weil

et al. 2013

Journal of Experimental Biology

Self Cite

View full text Add to dashboard Cite

SUMMARYCrustacean male sexual differentiation is governed by the androgenic gland (AG) and specifically by the secreted insulin-like AG hormone (IAG), thus far identified in several decapod species including the Australian red claw crayfish Cherax quadricarinatus (termed Cq-IAG). While a few insulin-like AG genes have been identified in crustaceans, other AG-specific genes have not been documented until now. In the present study, we describe the recent identification of a non-IAG AG-specific transcript obtained from the C. quadricarinatus AG cDNA library. This transcript, termed C. quadricarinatus membrane-anchored AG-specific factor (Cq-MAG), was fully sequenced and found to encode a putative product of 189 amino acids including a signal anchoring peptide. Expression of a recombinant GFP fusion protein lacking the signal anchor encoding sequence dramatically affected recombinant protein localization pattern. While the expression of the deleterious fusion protein was observed throughout most of the cell, the native GFP::Cq-MAG fusion protein was observed mainly surrounding the periphery of the nucleus, demonstrating an endoplasmic reticulum (ER)-like localization pattern. Moreover, co-expression of the wild-type Cq-MAG (fused to GFP) and the Cq-IAG hormone revealed that these peptides indeed co-localize. This study is the first to report a protein specifically associated with the insulin-like AG hormone in addition to the finding of another AG-specific transcript in crustaceans. Previous knowledge suggests that insulin/insulin-like factor secretion involves tissue-specific transcripts and membrane-anchored proteins. In this regard, Cq-MAGʼs tissue specificity, anchoring properties and intracellular co-localization with Cq-IAG suggest that it may play a role in the processing and secretion of this insulin-like AG hormone.

show abstract

The eyestalk–androgenic gland–testis endocrine axis in the crayfish Cherax quadricarinatus

Cited by 124 publications

References 34 publications

A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis

A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis

Neural Circuit Reconfiguration by Social Status

An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone

Contact Info

Product

Resources

About