The Extracts of Epilobium Parviflorum Inhibit MCF-7 Breast Cancer Cells

Kia, Bhenaz Hatefi; Noureini, Sakineh Kazemi; Kakhki, Mohammad Reza Vaezi

doi:10.32598/ijt.15.1.752.1

Cited by 5 publications

(4 citation statements)

References 8 publications

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“…Many cell viability studies using MTT assay provided evidence that anticancer properties reside in various plants such as the IC 50 value of Elaeis guineensis, Carthamus tenuis, Hyptis pectinata and Epilobium parviflorum is 15µg/ml, 25µg/ml, 46µg/ml, 73µg/ml, respectively. [13][14][15][16] Likewise, in the present investigation, IC 50 value of I. verum extract was found to be 5.5 ± 0.9µg/ml which caused significant reduction in MCF-7 cells viability, indicating its anticancer activity in a dosedependent manner (Table-I).…”

Section: Discussionsupporting

confidence: 67%

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Pahore¹,

Khan

Karim³

2022

Pak J Med Sci

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Objective: To investigate the anticancer effect of Illicium verum against human breast cancer MCF-7 cell line. Methods: An experimental study was conducted in Multidisciplinary and Tissue Culture Laboratory, Aga Khan University in collaboration with Pharmacology Department of Bahria University Medical and Dental College, Karachi, Pakistan from January 2021 to June 2021. MCF-7 cells of Luminal-A breast cancer were seeded in 96-well plate and treated with I.verum methanol extract. After incubation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye was used for cell viability and cell proliferation assays to determine the number of dead and viable cells, and the absorbance was measured using an enzyme-linked immunosorbent assay (ELISA) plate reader. In cell viability assay, different doses of I. verum methanol extract were used to treat the MCF-7 (0.25, 0.5, 1, 3, 6, 12, 25, and 50µg/ml) cells. For apoptosis analysis, the cells were processed with 4´, 6-diamidino-2-phenylindole fluorescent nuclear dye (DAPI) and were examined for fluorescence intensity and apoptotic cells. For cell proliferation assay and apoptosis the IC50 dose of 5.5µg/ml I. verum methanol extract was used. Results: The MCF-7 cells showed a significant reduction (p-value <0.01) in cell viability in the presence of all tested doses of I. verum methanol extract, except for the dose of 0.25µg/ml. The IC50 dose 5.5µg/ml of same extract also showed a significant reduction (p-value <0.01) in cell proliferation and apoptosis induction in MCF-7 cells. Conclusions: Illicium verum methanol extract possesses very potent anticancer action against MCF-7 cells through cytotoxicity, reduction, and inhibition of cancer cells and by inducing apoptosis. doi: https://doi.org/10.12669/pjms.39.1.6580 How to cite this: Pahore AK, Khan S, Karim N. Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line. Pak J Med Sci. 2023;39(1):---------. doi: https://doi.org/10.12669/pjms.39.1.6580 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionsupporting

confidence: 67%

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Pahore¹,

Khan

Karim³

2022

Pak J Med Sci

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“…The results obtained by Hatefi Kia et al in the experiments on normal (HEK293) and cancer (MCF7) cells proved high antiproliferative selective activity against tumor cells. Interestingly, methanolic extracts were more active than aqueous; moreover, the methanolic extracts derived from the plant roots were more active (IC 50 = 73 μg/mL) than those from the flowers and aerial parts (IC 50 equal 110 μg/mL and 160 μg/mL, respectively) [ 98 ]. Similarly, different activity between normal and cancer cells were observed in the human colon adenocarcinoma cell line (HT-29) and human colon epithelial cell line (CCD 841 CoTr) after their 96-h treatment with in vitro digested fireweed extract.…”

Section: Epilobium Angustifolium In Cosmetic and Dermatologi...mentioning

confidence: 99%

Epilobium angustifolium L. as a Potential Herbal Component of Topical Products for Skin Care and Treatment—A Review

et al. 2022

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Epilobium angustifolium L. (EA) has been used as a topical agent since ancient times. There has been an increasing interest in applying EA as a raw material used topically in recent years. However, in the literature, there are not many reports on the comprehensive application of this plant to skin care and treatment. EA contains many valuable secondary metabolites, which determine antioxidant, anti-inflammatory, anti-aging, and antiproliferative activity effects. One of the most important active compounds found in EA is oenothein B (OeB), which increases the level of ROS and protects cells from oxidative damage. OeB also influences wound healing and reduces inflammation by strongly inhibiting hyaluronidase enzymes and inhibiting COX-1 and COX-2 cyclooxygenases. Other compounds that play a key role in the context of application to the skin are flavonoids, which inhibit collagenase and hyaluronidase enzymes, showing anti-aging and anti-inflammatory properties. While terpenes in EA play an important role in fighting bacterial skin infections, causing, among other things cell membrane, permeability increase as well as the modification of the lipid profiles and the alteration of the adhesion of the pathogen to the animal cells. The available scientific information on the biological potential of natural compounds can be the basis for the wider use of EA in skin care and treatment. The aim of the article is to review the existing literature on the dermocosmetic use of E. angustifolium.

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“…is a well-known medicinal plant that grows naturally in many locations in the world, mainly in North America, Asia, and Europe ( Lin et al, 2022 ; Dreger et al, 2020 ) ( Kalle et al, 2020 ). The plant has well-known anti-inflammatory ( Ferrante et al, 2020 ) ( Nowak et al, 2021c ; Zagórska-Dziok et al, 2021 ), antibacterial, anticancer ( Kia et al, 2021 ; Ostrovska et al, 2017 ; Kowalik et al, 2022 ) ( Maruška et al, 2017 ) and antioxidant properties ( Lasinskas et al, 2020 ) ( Nowak et al, 2019 ; Karakaya et al, 2020 ). Traditionally, the leaves infusion of this plant is used as a remedy for headaches, colds, gastrointestinal and prostate disorders ( Lasinskas et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Anticancer properties of bacterial cellulose membrane containing ethanolic extract of Epilobium angustifolium L

Perużyńska

Nowak

Birger

et al. 2023

Front. Bioeng. Biotechnol.

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Epilobium angustifolium L. is a medicinal plant well known for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties related to its high polyphenols content. In the present study, we evaluated the antiproliferative properties of ethanolic extract of E. angustifolium (EAE) against normal human fibroblasts (HDF) and selected cancer cell lines, including melanoma (A375), breast (MCF7), colon (HT-29), lung (A549) and liver (HepG2). Next, bacterial cellulose (BC) membranes were applied as a matrix for the controlled delivery of the plant extract (BC-EAE) and characterized by thermogravimetry (TG), infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) images. In addition, EAE loading and kinetic release were defined. Finally, the anticancer activity of BC-EAE was evaluated against the HT-29 cell line, which presented the highest sensitivity to the tested plant extract (IC50 = 61.73 ± 6.42 µM). Our study confirmed the biocompatibility of empty BC and the dose and time-dependent cytotoxicity of the released EAE. The plant extract released from BC-2.5%EAE significantly reduced cell viability to 18.16% and 6.15% of the control values and increased number apoptotic/dead cells up to 37.53% and 66.90% after 48 and 72 h of treatment, respectively. In conclusion, our study has shown that BC membranes could be used as a carrier for the delivery of higher doses of anticancer compounds released in a sustained manner in the target tissue.

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The Extracts of Epilobium Parviflorum Inhibit MCF-7 Breast Cancer Cells

Cited by 5 publications

References 8 publications

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Epilobium angustifolium L. as a Potential Herbal Component of Topical Products for Skin Care and Treatment—A Review

Anticancer properties of bacterial cellulose membrane containing ethanolic extract of Epilobium angustifolium L

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