The extraction of phytol from Hydrilla verticillata using ultrasonic-assisted extraction (UAE), the analysis of antibacterial activity, and the utilization of residual extract for polyhydroxyalkanoate (PHA) production

Petpheng, Boossayarat; Mudtaleb, Bookree; Piboon, Watcharaphorn; Paichid, Nisa; Sangkharak, Kanokphorn

doi:10.1007/s13399-023-04331-5

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…The composition of the microbial population in the fish gut is influenced by diet, and phytochemicals and NICs have the potential to influence this structure, as they possess antimicrobial properties [53]. Phytol exhibits bactericidal properties against a diverse range of harmful bacteria, including Bacillus thermoamylovorans [54], Pseudomonas aeruginosa [55,56], Escherichia coli [57], and Clostridium sporogenes, Sarcina lutea, Enterococcus faecalis [58]. Phytol has the ability to induce oxidative stress, DNA damage, and membrane damage in bacterial cells [55,59], which explains the mechanism behind its antibacterial effect.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary phytol supplementation on growth performance, immunological parameters, intestinal bacteria, and prevention of oxidative stress following transportation of Nile tilapia, Oreochromis niloticus

Ahani,

Yousefi

et al. 2023

Preprint

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In this study, Nile tilapia, Oreochromis niloticus, fingerlings (2.00 ± 0.02) were fed diets (crude protein 345 g/kg; crude lipid 86.8 g/kg) containing 0 (CTL), 75 (PH-75), 150 (PH-150), and 300 (PH-300) mg/kg phytol for 60 days, at 4% of biomass. The fish were reared in sixteen 70-L tanks (n = 4, each with 40 fish). After 60 days rearing, the fish were subjected to 6-h transportation to monitor the hepatic antioxidant parameters. The results showed that dietary 75 mg/kg phytol significantly improved fish growth (P < 0.001) and feed efficiency (P = 0.015). Plasma lysozyme (P = 0.004) alternative complement (P = 0.006), alkaline phosphatase (P = 0.014) significantly increased in PH-75, 75PH/150PH, and PH-75/PH-300 treatments, respectively. All phytol-treatments led to significant (P = 0.001) increase in the blood neutrophil count. The PH-300 treatment resulted in significantly higher blood WBC (P = 0.008), monocyte (P = 0.010), and eosinophil (P < 0.001) counts, compared to CTL. Phytol also modulated intestinal microbiota by reducing the number of total viable bacteria in PH-75 and PH-150 (P = 0.027) and increasing lactic acid bacteria in PH-75 (P = 0.017). PH-75 and PH-150 had highest survival, antioxidant enzymes, reduced glutathione levels, lowest lipid peroxidation, before and/or after the transportation. PH-300 treatment showed an elevation lipid peroxidation, after the transportation. These findings suggest that dietary phytol may be a good supplement for improving the health and growth performance of Nile tilapia, and the recommended level is 75 mg/kg.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary phytol supplementation on growth performance, immunological parameters, intestinal bacteria, and prevention of oxidative stress following transportation of Nile tilapia, Oreochromis niloticus

Ahani,

Yousefi

et al. 2023

Preprint

View full text Add to dashboard Cite

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Effects of Dietary Phytol Supplementation on Growth Performance, Immunological Parameters, Intestinal Bacteria, and Prevention of Oxidative Stress Following Transportation of Nile Tilapia, Oreochromis niloticus

Ahani,

Yousefi

et al. 2024

Aquaculture Nutrition

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Nile tilapia, Oreochromis niloticus, (2.00 ± 0.02 g) were reared in 16 70-L tanks (40 individual/tank) and fed diets (approx. 345 g/kg protein, approx. 87 g/kg crude fat) containing 0 (CTL), 75 (PH-75), 150 (PH-150), and 300 (PH-300) mg/kg phytol (n = 4). After 60 days of feeding (4% daily), growth performance, humoral immune parameters, and gut bacteria were analyzed. Also, hepatic antioxidant parameters were determined before and after the fish were transported in plastic bags for 6 hr. The results showed that PH-75 exhibited the highest final weight (P<0.001), weight gain (P<0.001), feed intake (P<0.001), feed efficiency (P=0.015), plasma lysozyme activity (P=0.004), and intestinal Lactobacillus sp. population (P=0.017), among the treatments. The highest plasma alternative complement activity (P=0.006) and the lowest intestinal total viable bacteria (P=0.027) were observed in PH-75 and PH-150. The highest plasma alkaline phosphatase activities were observed in PH-75 and PH-300 (P=0.014). The highest blood leukocyte (P=0.008), monocyte (P=0.010), and eosinophil (P<0.001) were observed in PH-300, while the highest blood neutrophil was observed in all phytol treatments (P<0.001). The highest hepatic lipid peroxidation was observed in PH-300, whereas PH-75 and PH-150 showed the lowest values (P<0.001). The highest hepatic reduced glutathione was observed in PH-75, also PH-150 exhibited significant elevation in this parameter, compared to CTL (P<0.001). Transportation led to significant elevations in the hepatic antioxidant enzymes’ activities in CTL, PH-75, and PH-150; the highest activities were related to PH-75 and PH-150 treatments, which had also the highest post-transportation survivals (P<0.001). In conclusion, phytol is a suitable feed supplement for Nile tilapia, improving growth performance and welfare, particularly at 75 mg/kg.

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The extraction of phytol from Hydrilla verticillata using ultrasonic-assisted extraction (UAE), the analysis of antibacterial activity, and the utilization of residual extract for polyhydroxyalkanoate (PHA) production

Cited by 2 publications

References 31 publications

Effects of dietary phytol supplementation on growth performance, immunological parameters, intestinal bacteria, and prevention of oxidative stress following transportation of Nile tilapia, Oreochromis niloticus

Effects of dietary phytol supplementation on growth performance, immunological parameters, intestinal bacteria, and prevention of oxidative stress following transportation of Nile tilapia, Oreochromis niloticus

Effects of Dietary Phytol Supplementation on Growth Performance, Immunological Parameters, Intestinal Bacteria, and Prevention of Oxidative Stress Following Transportation of Nile Tilapia, Oreochromis niloticus

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