The Extract from Lysimachia foenum-graecum Induces Apoptosis in MCF-7 Breast Cancer Cells

Abstract

Abstract:The extract from Lysimachia foenum-graecum (LFE) has been known to possess various instructive characters including anti-oxidant, anti-obesity, fungicidal activities. However, the accurate mechanism of those effects of LFE is not well known. In that respect, we evaluated the apoptotic effect and anti-cancer efficacy of extracts of LFE in MCF-7 breast cancer cells. In this study, we hypothesized that LFE may exert cancer cell apoptosis through regulating p53 and mitochondria-mediated apoptotic proteins. And this substance can generate ROS to cause free radical-induced apoptosis. Accordingly, the generation of ROS by LFE triggers the activation of p53 which are accompanied by pro-apoptotic protein activation and suppression of pro-survival proteins. We determined with MTT assay, flow cytometry for detection of intracellular ROS and Annexin V-PI staining, Western blotting. Consequently, our researches demonstrated that the treatment of LFE to breast cancer cells resulted in an activation of p53, Puma, Bax, cleaved-PARP and an inhibition of Bcl-2 expressions.

Apoptotic effects of extract from Cnidium monnieri (L.) Cusson by adenosine monosphosphate-activated protein kinase-independent pathway in HCT116 colon cancer cells

“…Cancer is caused by the abnormal proliferation of cells, and interference with cell proliferation as well as induction of cancer cell death have been widely employed in the treatment of cancer (6). Colon cancer is of particular interest in South Korea, as its prevalence has increased with the adaptation of Western eating habits (1,2).…”

“…Apoptosis signaling is regulated by molecules including AMPK and Akt in response to environmental changes (5,8,12). Cancer cells regulate the activation of several signaling molecules, such as pro-survival factors, to stimulate cell growth and proliferation, and to protect themselves (5,6). The induction of apoptosis through the control of pro-survival and pro-apoptotic factors been extensively studied (1,4,6).…”

“…Cancer cells require large amounts of energy and molecules, including lipids and amino acids, for cell growth and proliferation and to protect themselves through the regulation of pro-survival factors (5). The number of studies on the potential use of natural plant extracts for the inhibition of cancer-cell proliferation and metastasis through the regulation of pro-survival factors has increased (4)(5)(6). A number of previous studies have shown that various active components of herbal medicines, such as curcumin, as well as selenium, induce apoptosis through the regulation of AMP-activated protein kinase (AMPK) and Akt in colon cancer cells (4,5,7).…”

Apoptotic Effects and Cell Cycle Arrest Effects of Extracts from Cnidium monnieri (L.) Cusson through Regulating Akt/mTOR/GSK-3β Signaling Pathways in HCT116 Colon Cancer Cells

“…그러나 이러한 방법은 항암제 내성이 나 면역력 저하와 같은 부작용을 초래한다는 문제점이 있다 [7]. 이러한 이유로 최근에는 천연물 자체의 효능을 기반으로 항암효과가 있는 신규생리활성물질을 개발하기 위하여 많은 연구가 진행되고 있으며, 천연 추출물이 암세포의 성장과 증 식에 관련된 신호전달 분자를 조절함으로써 암을 억제한다는 선행연구들이 보고되고 있다 [11,14,19,20].…”

Torilis japonica extract, a new potential EMT suppressor agent by regulation of EGFR signaling pathways

“…In particular, attention has been paid to herbs that are used in eastern medicine. Recently research has found that herb medicines are effective in inhibiting cancer proliferation and invasion and have fewer side-effects than existing drugs (26,27).…”