The Extracellular Matrix of the Fetal Wound: Hyaluronic Acid Controls Lymphocyte Adhesion

Dillon, Peter W.; Keefer, Kerry; Blackburn, James H.; Houghton, Pamela E.; Krummel, Thomas M.

doi:10.1006/jsre.1994.1126

Cited by 20 publications

(9 citation statements)

References 20 publications

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“…Lymphocytes are found in the wound at the very early phase of wound healing 21,22 and they are shown to take part in the wound healing process 28–30 . Dillon et al demonstrated that fetal adhesion of lymphocytes to HA was minimal, which may prevent an inflammatory response in fetal wounds 31 . In our study, there was a significant positive correlation between HA concentration and wound lymphocytes.…”

Section: Discussionsupporting

confidence: 58%

Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children

Pajulo¹,

Pulkki²,

Lertola³

et al. 2001

Wound Repair Regeneration

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When inserted into a human incision wound, the Cellstick device harvests inflammatory cells and collects wound fluid, reflecting time-related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing and in scarless fetal wound healing. The aim of this work was to determine the concentration and variation of hyaluronic acid and proportions of wound cells in closed surgical wounds in children at two time points. The Cellstick device was inserted subcutaneously into the wound at the end of an elective inguinal hernia operation on 37 healthy boys, and the devices were removed 3+/-1 or 24+/-3 hours after surgery. Haluronic acid concentration was measured from the wound fluid and a differential count of the wound cells was performed. There was a significant decrease in hyaluronic acid concentration from 3+/-1 to 24+/-3 hours after surgery (p<0.001, Kruskal-Wallis anova). The variance of hyaluronic acid concentration in wound fluid differed between the wounds at the two time points (p<0.01, Levene test for homogeneity of variance). A positive correlation between hyaluronic acid concentration and patient age (r=0.91, p<0.05, Spearman) at 3+/-1 hours post surgery and between HA and wound lymphocytes (r=0.38, p<0.05, Spearman) was also found. We conclude that the hyaluronic acid concentration in wound fluid peaks early in children and decreases significantly by 3 to 24 hours after surgery, and the concentrations in the wound fluid of healthy boys are more variable 3 hours than at 24 hours after surgery.

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Section: Discussionsupporting

confidence: 58%

Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children

Pajulo¹,

Pulkki²,

Lertola³

et al. 2001

Wound Repair Regeneration

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“…41,42 The high HA concentrations have been linked to the minimal inflammatory response found in fetal wound healing by disrupting platelet adhesion and degranulation. 43,44 The elevated HA levels may also influence the amount of collagen deposited, 45,46 the nature of fibrils formed, 47 and the lack of scar formation. 35 The distribution of CS proteoglycans is also different in fetal wounds, 48 but their influence on wound healing has yet to be determined.…”

Section: Discussionmentioning

confidence: 98%

Glycosaminoglycan Hydrogels as Supplemental Wound Dressings for Donor Sites

Kirker

Luo

Morris

et al. 2004

The Journal of Burn Care &Amp; Rehabilitation

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Chemically crosslinked glycosaminoglycan (GAG) hydrogel films were evaluated as biointeractive dressings in a porcine model for donor-site autograft wounds. Multiple 5 x 5 x 0.03 cm wounds were created on the dorsum of pigs. Half of the wounds were treated with a GAG film plus an occlusive dressing (Tegaderm), whereas the other half were treated with Tegaderm alone. At 3, 5, or 7 days after surgery, the partially healed wounds were excised and evaluated histologically for three animals at each time point. By day 3, epithelial cells had proliferated and migrated from wound edges and from epithelial islands associated with residual hair follicles to begin to cover the wound bed. A statistically significant increase in coverage was observed for GAG + Tegaderm-dressed wounds than for those with Tegaderm alone at day 3 and day 5 post-surgery. By day 7, all treatment groups were completely healed. Thus, GAG hydrogels accelerated wound healing by enhancing re-epithelialization.

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“…32,33 Hyaluronic acid acts advantageously for cellular migration and seems to inhibit inflammation, thus it regulates the microenvironment advantageously for fetal cutaneous regeneration. 34,35 Hyaluronic acid up-regulates type III collagen and TGF-β3 expression. These results suggest that TGF-β3 may promote a fetal-like cell environment that favors scarless healing.…”

Section: Molecular Differences (1) Growth Factorsmentioning

confidence: 99%

Fetal Skin Possesses the Ability to Regenerate Completely: Complete Regeneration of Skin

et al. 2012

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Until a certain developmental stage, cutaneous wounds in mammalian fetuses heal rapidly without scars with complete regeneration of the skin. In the process of fetal wound healing, inflammatory responses, granulation proliferation, and scar formation that are observed in adults are not seen. Numerous studies have reported the causes of fetal scarless cutaneous regeneration, including reduced expression of TGF-β1 and higher levels of hyaluronan in the extracellular matrix, from the viewpoints of molecular biology and cellular biology, but the mechanisms are not completely understood. Although a variety of substances that inhibit scar formation have been investigated, currently it is almost impossible for adult cutaneous wounds to heal completely without scars. Except for a few animal species, perfect regeneration after wounding can occur only during the gestation period. By strictly comparing the stages before and after the transition from the regeneration of skin to scarring, it will be possible to investigate the mechanisms of cutaneous regeneration.

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The Extracellular Matrix of the Fetal Wound: Hyaluronic Acid Controls Lymphocyte Adhesion

Cited by 20 publications

References 20 publications

Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children

Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children

Glycosaminoglycan Hydrogels as Supplemental Wound Dressings for Donor Sites

Fetal Skin Possesses the Ability to Regenerate Completely: Complete Regeneration of Skin

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