The extracellular matrix of human bone marrow adipocytes and glucose concentration differentially alter mineralization quality without impairing osteoblastogenesis

Entz, Laura; Falgayrac, Guillaume; Chauveau, Christophe; Pasquier, Gilles; Lucas, Stéphanie

doi:10.1016/j.bonr.2022.101622

Bone Reports

2022

DOI: 10.1016/j.bonr.2022.101622

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The extracellular matrix of human bone marrow adipocytes and glucose concentration differentially alter mineralization quality without impairing osteoblastogenesis

Laura Entz

Guillaume Falgayrac

Christophe Chauveau

et al.

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Cited by 2 publications

References 90 publications

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VK2 Promotes Osteogenic Differentiation of BMSCs against High Glucose Exposure via Modulation of Intracellular Oxidative Stress

Chen

Fang

et al. 2023

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INTRODUCTION: Diabetic osteoporosis (DOP) has gradually gained public attention. The clinical manifestations of DOP include bone mass loss, bone microstructural damage, and increased bone fragility. background: Diabetic osteoporosis (DOP) has gradually gained public attention. The clinical manifestations of DOP include bone mass loss, bone microstructural damage and increased bone fragility. Intracellular reactive oxygen species (ROS) production was significantly increased under high glucose (HG) conditions, with deleterious effects on bone mesenchymal stem cells (BMSCs) proliferation and osteogenic differentiation. Vitamin K2 (VK2) has been demonstrated to promote bone formation both in vitro and in vivo. However, its potential role in diabetes-induced osteoporosis remains unelucidated. METHOD: Intracellular reactive oxygen species (ROS) production was significantly increased under high glucose (HG) conditions, with deleterious effects on bone mesenchymal stem cells (BMSCs) proliferation and osteogenic differentiation. Vitamin K2 (VK2) has been demonstrated to promote bone formation both in vitro and in vivo. RESULT: However, its potential role in diabetes-induced osteoporosis remains unelucidated. This study aims to verify whether VK2 treatment could relieve the deleterious effects of high glucose on BMSCs and delay the progression of osteoporosis. The results revealed that the HG environment downregulated the expression of osteogenesis-related proteins. method: The protective effect of VK2 on BMSCs were evaluated by CCK-8, the mitochondrial function was detected by mitosox and JC-1. Osteogenic diferentiation potential was examined by alkaline phosphatase (ALP) staining and alizarin red s (ARS) staining.Simultaneously, we used an established diabetes-induced osteoporosis rat madel to assess the effects of VK2 in vivo. CONCLUSION: Correspondingly, VK2 treatment reversed the osteogenic phenotype of BMSCs under HG conditions. In addition, using an established diabetes-induced osteoporosis rat model, we found that VK2 administration could restore bone mass and microstructure. In conclusion, our results provide a promising therapeutic option in the clinical treatment of DOP. other: NO

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VK2 Promotes Osteogenic Differentiation of BMSCs against High Glucose Exposure via Modulation of Intracellular Oxidative Stress

Chen

Fang

et al. 2023

CPD

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Fabrication of Magnetic Poly(L-lactide) (PLLA)/Fe3O4 Composite Electrospun Fibers

Liu,

Zheng,

Lin

et al. 2024

Materials

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Electrospinning technology is widely used for preparing biological tissue engineering scaffolds because of its advantages of simple preparation, accurate process parameters, and easy control. Poly(L-lactide) (PLLA) is regarded as a promising biomass-based polymer for use in electrospinning. The incorporation of Fe3O4 nanoparticles (NPs) could improve the osteogenic differentiation and proliferation of cells in the presence or absence of a static magnetic field (SMF). In this work, these two materials were blended together to obtain electrospun samples with better dispersibility and improved magnetic properties. First, composite PLLA and Fe3O4 NP fibers were prepared by means of electrospinning. The influence of electrospinning conditions on the morphology of the composite fibers was then discussed. Changes in magnetic properties and thermal stability resulting from the use of different PLLA/Fe3O4 mass ratios were also considered. Next, the morphology, crystal state, thermodynamic properties, and magnetic properties of the electrospun samples were determined using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and vibration sample magnetization (VSM). The results showed that the fibers prepared using PLLA with Mn = 170,000 exhibited good morphology when electrospun at 12 KV. The magnetic properties of PLLA/Fe3O4 composite electrospun fibers increased with the NP content, with the exception of thermal stability. The results of the present study may help to promote the further development of PLLA/Fe3O4 composite materials in the biomedical field.

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The extracellular matrix of human bone marrow adipocytes and glucose concentration differentially alter mineralization quality without impairing osteoblastogenesis

Cited by 2 publications

References 90 publications

VK2 Promotes Osteogenic Differentiation of BMSCs against High Glucose Exposure via Modulation of Intracellular Oxidative Stress

VK2 Promotes Osteogenic Differentiation of BMSCs against High Glucose Exposure via Modulation of Intracellular Oxidative Stress

Fabrication of Magnetic Poly(L-lactide) (PLLA)/Fe3O4 Composite Electrospun Fibers

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