The expression ratio of miR-17-5p and miR-155 correlates with grading in canine splenic lymphoma

Albonico, F.; Mortarino, Michele; Avallone, Giancarlo; Gioia, G.; Comazzi, S.; Roccabianca, P.

doi:10.1016/j.vetimm.2013.06.018

Cited by 19 publications

(18 citation statements)

References 27 publications

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“…At least 4 mismatches distinguished the chosen miRNA candidates from the closest dog or vertebrate miRNA homologue identified using miRBase and BLASTn [42]. One host miRNA (cfa-miR-223, not present in D. immitis or B. pahangi ) served as control [43], [44]. An optimized stem-loop RT-qPCR protocol for miRNAs was employed for primer design and experiments [45].…”

Section: Methodsmentioning

confidence: 99%

Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections

et al. 2014

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Filarial nematodes cause chronic and profoundly debilitating diseases in both humans and animals. Applications of novel technology are providing unprecedented opportunities to improve diagnosis and our understanding of the molecular basis for host-parasite interactions. As a first step, we investigated the presence of circulating miRNAs released by filarial nematodes into the host bloodstream. miRNA deep-sequencing combined with bioinformatics revealed over 200 mature miRNA sequences of potential nematode origin in Dirofilaria immitis-infected dog plasma in two independent analyses, and 21 in Onchocerca volvulus-infected human serum. Total RNA obtained from D. immitis-infected dog plasma was subjected to stem-loop RT-qPCR assays targeting two detected miRNA candidates, miR-71 and miR-34. Additionally, Brugia pahangi-infected dog samples were included in the analysis, as these miRNAs were previously detected in extracts prepared from this species. The presence of miR-71 and miR-34 discriminated infected samples (both species) from uninfected samples, in which no specific miRNA amplification occurred. However, absolute miRNA copy numbers were not significantly correlated with microfilaraemia for either parasite. This may be due to the imprecision of mf counts to estimate infection intensity or to miRNA contributions from the unknown number of adult worms present. Nonetheless, parasite-derived circulating miRNAs are found in plasma or serum even for those species that do not live in the bloodstream.

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Section: Methodsmentioning

confidence: 99%

Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections

et al. 2014

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“…This suggestion of a dual role for miRNA-17-5p has been previously made in human breast cancer [ 34 ]. Another study reported the ability of miRNA-17-5p to differentiate between high- and low-grade samples of canine splenic lymphomas, with intermediate/high-grade samples expressing significantly higher levels of miRNA-17-5p [ 2 ]. This example demonstrates that a miRNA can be a tumor suppressor or an oncomiR depending on the mRNA whose translation the miRNA is regulating.…”

Section: Microrna Expression and Regulation In Canine And Human Tumormentioning

confidence: 99%

“…The ability to inhibit tumor cell growth or invasion following ectopic expression of down-regulated miRNAs indicates the value of this class of non-coding RNAs in the treatment of canine and human lymphoma and leukemias. miRNA-155 was down-regulated in canine splenic lymphomas [ 2 ] and in intermediate/high-grade samples compared with low-grade samples. In contrast, overexpression of miRNA-17-5p was correlated with the mitotic index of the tissues, suggesting the use of miRNA-17-5p (increased expression) and miRNA-155 (low to absent expression) as potential markers for canine lymphomas.…”

Section: Microrna Expression and Regulation In Canine And Human Tumormentioning

confidence: 99%

Comparative aspects of microRNA expression in canine and human cancers

et al. 2018

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MicroRNAs (miRNAs) have important roles in all biological pathways in multicellular organisms. Over 1,400 human miRNAs have been identified, and many are conserved among vertebrates and invertebrates. Regulation of miRNA is the most common mode of post-transcriptional gene regulation. The miRNAs that are involved in the initiation and progression of cancers are termed oncomiRs and several of them have been identified in canine and human cancers. Similarly, several miRNAs have been reported to be down-regulated in cancers of the two species. In this review, current information on the expression and roles of miRNAs in oncogenesis and progression of human and canine cancers, as well the roles miRNAs have in cancer stem cell biology, are highlighted. The potential for the use of miRNAs as therapeutic targets in personalized cancer therapy in domestic dogs and their possible application in human cancer counterparts are also discussed.

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“…Thus far, limited molecular studies in companion animals have demonstrated the utility of examining gene signatures of tumors to discover potential biomarkers of outcome and treatment. [1][2][3][4][5] However, in clinical veterinary practice use of genomic information for supportive diagnostics remains unfulfilled. A complimentary approach using gene expression profiling is highly useful in studies of human cancers and can be extrapolated to cancers in dogs.…”

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confidence: 99%

Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin‐Fixed Paraffin‐Embedded Tissues

Davis¹,

Schwartz

Duchemin³

et al. 2017

Veterinary Internal Medicne

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BackgroundUse of molecular‐based diagnostics for companion animals is impeded by availability of technology platforms, tissue acquisition requirements, and species‐specific reagents.Hypothesis/ObjectivesTo validate a quantitative nuclease protection assay (qNPA) to simultaneously measure RNA expression of multiple genes in archived formalin‐fixed paraffin‐embedded (FFPE) tumors from dogs.AnimalsAll tumor biopsy samples were collected retrospectively from surgical biopsies and in the care of veterinarians.MethodsRetrospective case series. A qNPA 96‐well ArrayPlate was built using 30 canine‐specific genes, 5 housekeeping genes, positive and negative controls with qualified gene‐specific oligonucleotides. Pearson's correlation, coefficient of variation (CV), and multivariate analysis were used to determine analytical performance using 40 FFPE dog tumors. Once validated, 70 FFPE dog tumors were analyzed for differences in gene expression using hierarchical clustering and analysis of variance of log transformed data. Immunohistochemistry (IHC) was performed to correlate gene expression and protein expression in a subset of tumors.ResultsThe assay was linear with decreasing sample input (R 2 = 0.978), reproducible within and between 96‐well plates (r = 0.988 and 0.95, respectively) and between different laboratories (CV = 0.96). Hierarchical cluster analysis showed grouping of tumors by histogenesis and oncogenes. Significant differences were found between BCl2, E2F transcription factor 1, MDM2, COX‐2, MET proto‐oncogene receptor kinase, and other biologically relevant gene expression in tumor subtypes. Immunohistochemistry confirmed protein expression.Conclusions and Clinical ImplicationsBecause this technology works reliably on FFPE specimens, it can help expedite the broad introduction of multiplexed genomic information for improved diagnostics and discovery of new targets for therapies in veterinary oncology.

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The expression ratio of miR-17-5p and miR-155 correlates with grading in canine splenic lymphoma

Cited by 19 publications

References 27 publications

Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections

Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections

Comparative aspects of microRNA expression in canine and human cancers

Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin‐Fixed Paraffin‐Embedded Tissues

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