The expression patterns of Tetratricopeptide repeat domain 36 (Ttc36)

Zhou, Yuru; He, Qi; Chen, Jihui; Liu, Yunhong; Mao, Zhizhong; Lyu, Zhong-Shi; Ni, Dongsheng; Long, Yaoshui; Ju, Pan; Liu, Jianing; Gu, Yue; Zhou, Qin

doi:10.1016/j.gep.2016.11.001

Cited by 15 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TTC36, a kidney gene marker that aligns to both the mouse and human genome, is represented in each cluster with low gene counts in all graft samples. To verify, we generated violin plots to show upregulated expression of TTC36 in the identified low gene count population ( Figure S1E ; Table S1 ) ( Zhou et al, 2016 ). For all grafted samples, we removed all cells enriched with TTC36 as a method to distinguish mouse cells and remove them from subsequent analysis.…”

Section: Resultsmentioning

confidence: 99%

Single-Cell Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

et al. 2020

View full text Add to dashboard Cite

SUMMARY Human pluripotent stem cells differentiated to insulin-secreting β Cells (SC-β Cells) in islet organoids could provide an unlimited cell source for diabetes cell replacement therapy. However, current SC-β Cells generated in vitro are transcriptionally and functionally immature compared to native adult β Cells. Here, we use single-cell transcriptomic profiling to catalog changes that occur in transplanted SC-β Cells. We find that transplanted SC-β Cells exhibit drastic transcriptional changes and mature to more closely resemble adult β Cells. Insulin and IAPP protein secretions increase upon transplantation, along with expression of maturation genes lacking with differentiation in vitro , including INS , MAFA , CHGB , and G6PC2 . Other differentiated cell types, such as SC-α and SC-enterochromaffin (SC-EC) cells, also exhibit large transcriptional changes. This study provides a comprehensive resource for understanding human islet cell maturation and provides important insights into maturation of SC-β Cells and other SC-islet cell types to enable future differentiation strategy improvements.

show abstract

Section: Resultsmentioning

confidence: 99%

Single-Cell Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To determine the mechanism underlying regulation of HPD expression via posttranslational modifications, we immunoprecipitated HPD from HEK293T cells. Mass spectrometry analyses revealed that molecular chaperone TTC36, a tetratricopeptide repeat (TPR) motif family member 11,12 , is an HPD-associated protein (Supplementary Fig. 1b).…”

Section: Resultsmentioning

confidence: 99%

HPD degradation regulated by the TTC36-STK33-PELI1 signaling axis induces tyrosinemia and neurological damage

Xie

et al. 2019

Nat Commun

Self Cite

View full text Add to dashboard Cite

Decreased expression of 4-hydroxyphenylpyruvic acid dioxygenase (HPD), a key enzyme for tyrosine metabolism, is a cause of human tyrosinemia. However, the regulation of HPD expression remains largely unknown. Here, we demonstrate that molecular chaperone TTC36, which is highly expressed in liver, is associated with HPD and reduces the binding of protein kinase STK33 to HPD, thereby inhibiting STK33-mediated HPD T382 phosphorylation. The reduction of HPD T382 phosphorylation results in impaired recruitment of FHA domain-containing PELI1 and PELI1-mediated HPD polyubiquitylation and degradation. Conversely, deficiency or depletion of TTC36 results in enhanced STK33-mediated HPD T382 phosphorylation and binding of PELI1 to HPD and subsequent PELI1-mediated HPD downregulation. Ttc36−/− mice have reduced HPD expression in the liver and exhibit tyrosinemia, damage to hippocampal neurons, and deficits of learning and memory. These findings reveal a previously unknown regulation of HPD expression and highlight the physiological significance of TTC36-STK33-PELI1-regulated HPD expression in tyrosinemia and tyrosinemia-associated neurological disorders.

show abstract

“…The present study revealed that the Ttc38 associated with folic acid was also negatively correlated with the kidney. Another tetratricopeptide repeat (TPR) member, Ttc36, has an organ-specific expression profile, and shows a high expression level in the kidneys and liver, and a low expression level in the testis [ 51 ]. The expression of Ttc36 in renal proximal tubules is spatially and temporally-specific [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…Another tetratricopeptide repeat (TPR) member, Ttc36, has an organ-specific expression profile, and shows a high expression level in the kidneys and liver, and a low expression level in the testis [ 51 ]. The expression of Ttc36 in renal proximal tubules is spatially and temporally-specific [ 51 ]. A recent proteome of mouse with experimental autoimmune encephalomyelitis (EAE) identified the downregulation of Ttc38 in the brain [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Novel genes associated with folic acid-mediated metabolism in mouse: A bioinformatics study

Zhao

Zou

2020

PLoS ONE

View full text Add to dashboard Cite

Folic acid plays an essential role in the central nervous system and cancer. This study aimed to screen genes related to folic acid metabolism. Datasets (GSE80587, GSE65267 and GSE116299) correlated to folic acid were screened in the Gene Expression Omnibus. Weighed gene co-expression network analysis was performed to identify modules associated with sample traits of folic acid and organs (brain, prostate and kidney). Functional enrichment analysis was performed for the eigengenes in modules that were significantly correlated with sample traits. Accordingly, the hub genes and key nodes in the modules were identified using the protein interaction network. A total of 17,252 genes in three datasets were identified. One module, which included 97 genes that were highly correlated with sample traits (including folic acid treatment [cor =-0.57, P = 3e-04] and kidney [cor =-0.68, p = 4e-06]), was screened out. Hub genes, including tetratricopeptide repeat protein 38 (Ttc38) and miR-185, as well as those (including Sema3A, Insl3, Dll1, Msh4 and Snai1) associated with "neuropilin binding", "regulation of reproductive process" and "vitamin D metabolic process", were identified. Genes, including Ttc38, Sema3A, Insl3, Dll1, Msh4 and Snai1, were the novel factors that may be associated with the development of the kidneys and related to folic acid treatment.

show abstract

The expression patterns of Tetratricopeptide repeat domain 36 (Ttc36)

Cited by 15 publications

References 23 publications

Single-Cell Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

Single-Cell Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

HPD degradation regulated by the TTC36-STK33-PELI1 signaling axis induces tyrosinemia and neurological damage

Novel genes associated with folic acid-mediated metabolism in mouse: A bioinformatics study

Contact Info

Product

Resources

About