The expression patterns of mRNA‐encoding stem cell factor, internal stem cell factor and c‐kit in the prepubertal and adult porcine ovary

Brankin, Victoria; Hunter, M. G.; Horan, Tracey L; Armstrong, David G.; Webb, Robert I.

doi:10.1111/j.0021-8782.2004.00339.x

Cited by 22 publications

(14 citation statements)

References 34 publications

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“…The highest expression of KL in the primordial follicles in sheep in the present study signifies its role in the activation and growth of the primordial follicle as in the murine (Nilsson & Skinner, 2004;Parrott & Skinner, 1999). Kit ligand was expressed in the cumulus cells isolated from all the in vivo stages ( Table 2) the ovarian follicles, which is in agreement with previous reports in primordial follicles in cortical slices and primary follicles in goat (Silva et al, 2006), primordial, primary and antral follicles in sheep (Tisdall et al 1997), mouse ovarian follicles (Ye et al, 2009), some stages of ovarian follicles in women (David et al, 2012;Tuck et al, 2015) and granulosa cells in pig (Brankin, Hunter, Horan, Armstrong, & Webb, 2004;and Okamura et al, 2001). In line with a previous report (Parrish et al, 2011), the expression of KL did not show any significant changes in the cumulus cells as the preantral follicles developed into antral follicles.…”

Section: Discussionsupporting

confidence: 90%

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Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Srividya

Chakravarthi

Kona

et al. 2017

Reprod Domestic Animals

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Expression of Kit ligand (KL) and insulin-like growth factor binding protein (IGFBP3) genes was studied at different in vivo and corresponding in vitro stages of development of the ovarian follicles in sheep. The expression of both KL and IGFBP3 was significantly higher in the primordial follicles relative to any other stage of development. Compared to the other stages, the KL expression in the cumulus cells from in vivo grown large antral follicles and that of IGFBP3 in COCs' isolated from large antral follicles matured in vitro for 24 hr were significantly higher. In the oocytes from in vivo grown ovarian follicles, the expression of KL was the same at all the stages of development. Insulin-like growth factor binding protein 3 expression was also the same in the oocytes at all the stages of the development except for a significantly lower expression in those from antral follicles. The expression of KL in the cumulus cells decreased significantly in the in vitro grown early antral follicles but did not change further as the development progressed. The expression of IGFBP3 in the cumulus cells from in vitro grown ovarian follicles appeared to increase as the development progressed although the increase was not significant between any two consecutive stages of development. In the oocytes in in vitro grown ovarian follicles, the expression levels of KL and IGFBP3 genes did not change with development. It is concluded that (i) KL and IGFBP3 genes follow specific patterns of expression during ovarian folliculogenesis and (ii) in vitro culture of preantral follicles compromises the development potential through alterations in the stage-specific patterns of expression of these and other developmentally important genes.

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Section: Discussionsupporting

confidence: 90%

“…() and Brankin et al. () in preovulatory follicles of pig and David et al. () in primordial, primary, secondary and antral follicles in women.…”

Section: Discussionmentioning

confidence: 97%

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Srividya

Chakravarthi

Kona

et al. 2017

Reprod Domestic Animals

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“…The expression of KIT mRNA and KIT protein has been detected in the primordial, growing and full-grown oocytes of the mouse [8]. The mRNA encoding KL and KIT has also been detected in the porcine ovary; KL mRNA was localized in granulosa cells and KIT mRNA was detected in oocytes [19,20]. In the present study, KIT was expressed in primordial oocytes from both neonatal and prepubertal pigs.…”

Section: Discussionsupporting

confidence: 60%

KIT-KIT Ligand in the Growth of Porcine Oocytes in Primordial Follicles

Moniruzzaman

Miyano

2007

Journal of Reproduction and Development

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Abstract. Mammalian ovaries are endowed with a huge number of small oocytes in primordial follicles (primordial oocytes). The mechanism regulating initiation of oocyte growth and follicular development is not well understood. Several growth factors and cytokines are known to be involved in oocyte growth and follicular development. Herein, the involvement of KIT, a receptor tyrosine kinase, and its ligand, KIT ligand (KL), in the initiation of porcine oocyte growth was examined. At first, KIT expression was examined immunohistochemically in primordial oocytes from neonatal (10-20 days) and prepubertal (about 6 months) pigs. Similar expression of KIT was detected in all oocytes from both the neonatal and prepubertal pigs. Next, to examine the growth of primordial oocytes, ovarian tissues containing primordial oocytes were xenotransplanted into immunodeficient SCID mice. Primordial oocytes from the neonatal pigs grew with follicular development as described previously, whereas those from the prepubertal pigs did not initiate growth in the xenografts after 2 months. To stimulate the growth of primordial oocytes from the prepubertal pigs, they were cultured in a medium supplemented with KL (50 and 100 ng/ml) for 1 or 3 days before xenografting. After 2 months, however, the oocytes did not grow, and the primordial follicles did not develop, although a higher number of primordial oocytes survived in the KL-treated tissues. These results suggest that KIT-KL might not be associated with the growth initiation of porcine primordial oocytes, although they do enhance the survival of the oocytes.

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“…The expression of KIT mRNA and KIT protein has been detected in primordial, growing and full-grown oocytes in the mouse [31]. The mRNA encoding KIT and KL was detected in the porcine ovary, in which KL mRNA was localized in granulosa cells and KIT mRNA was detected in oocytes [34,35]. In our study [24], KIT was expressed in all primordial oocytes from both neonatal and prepubertal pigs.…”

Section: Stimulatory Factors Of Primordial Oocytessupporting

confidence: 51%

Growth of Primordial Oocytes in Neonatal and Adult Mammals

Moniruzzaman

Miyano

2010

J. Reprod. Dev.

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Abstract. Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. A small number of primordial oocytes start to grow, while others remain quiescent. Little is known about the mechanism regulating the activation of primordial oocytes. Recently, we found that primordial follicles in mature cows and prepubertal pigs took longer to initiate growth in xenografts compared with those in neonatal animals. We think that primordial oocytes in adult mammals are different from those in neonatal mammals. In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. The dormancy of primordial oocytes may be required to reserve the non-growing oocyte pool for the long reproductive life in mammals. FOXO3 is considered one of the molecules responsible for the dormancy of primordial oocytes in adult ovaries. These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. Key words: Adult, Neonatal, Oocyte growth, Ovary, Primordial follicle (J. Reprod. Dev. 56: [559][560][561][562][563][564][565][566] 2010) n fetal ovaries, germ cells known as oogonia proliferate mitotically and become oocytes that enter the meiotic cell cycle, and the oocytes then become arrested at prophase I. At around birth in rodents and during mid-gestation in humans and pigs, oocytes (15-20 μm in diameter in rodents and 30 μm in diameter in humans and pigs) are enclosed by a single layer of flattened follicular epithelial cells (granulosa cells; Fig. 1). This unit consisting of an oocyte and granulosa cells is called the primordial follicle. The oocytes in primordial follicles are called "primordial oocytes" [1]. After formation of primordial follicles, some of the primordial oocytes begin to grow, although most spend months or years in the quiescent state. Activation of primordial oocytes causes the transformation of their surrounding granulosa cells to a cuboidal shape [2]. The follicles at this stage are called primary follicles that contain growing oocytes. The granulosa cells proliferate and become multilayered to form secondary follicles. As follicles develop through the primary, secondary and tertiary (antral) stages, they gain successive layers of granulosa cells, oocytes increase in size towards 70-75 μm in rodents and 120-125 μm in humans, cows and pigs and theca cell layers surround the follicles. Finally, a large fluid-filled antral cavity (follicular antrum) is formed, and the follicle is called a tertiary or antral follicle.The time point at which first activation of primordial oocytes occurs depends on the species. In mice, growth of a portion of oocytes begins within several days after birth [3,4]...

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The expression patterns of mRNA‐encoding stem cell factor, internal stem cell factor and c‐kit in the prepubertal and adult porcine ovary

Cited by 22 publications

References 34 publications

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

KIT-KIT Ligand in the Growth of Porcine Oocytes in Primordial Follicles

Growth of Primordial Oocytes in Neonatal and Adult Mammals

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