The expression patterns and putative function of nitrate transporter 2.5 in plants

Liu, Ranran; Jia, Ting; Cui, Bing; Song, Jie

doi:10.1080/15592324.2020.1815980

Cited by 17 publications

(15 citation statements)

References 78 publications

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“…In Com5_NRT2, orthologues of AtNRT2.5 were found in the monocots O. sativa (OsNRT2.3) [ 25 , 95 ], H. vulgare (HvNRT2.1) [ 35 ], and Z. may (ZmNRT2.5) [ 34 ], differing in genomic context with from their A. thaliana orthologue, which was identified in Com3NRT2 ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

“…For monocots, this gene, in addition to being expressed in roots and leaves, as in eudicots, is also expressed in embryos and shells in wheat [ 57 , 96 ] and in silk, cobs, and tassel husk leaves in corn [ 34 , 97 ]. Eudicot expression has only been found at extremely low levels in dried Arabidopsis seeds [ 18 ] but not in poplar [ 32 ], teatree [ 98 ], or cassava [ 25 ]. Thus, in addition to the important role of NRT2.5 in Arabidopsis during N starvation and absorption of nitrate at very low concentrations, in monocots, it plays a fundamental role in the accumulation of nitrate in the seed and the filling of the grain [ 25 , 34 , 57 , 99 ].…”

Section: Discussionmentioning

confidence: 99%

“…Eudicot expression has only been found at extremely low levels in dried Arabidopsis seeds [ 18 ] but not in poplar [ 32 ], teatree [ 98 ], or cassava [ 25 ]. Thus, in addition to the important role of NRT2.5 in Arabidopsis during N starvation and absorption of nitrate at very low concentrations, in monocots, it plays a fundamental role in the accumulation of nitrate in the seed and the filling of the grain [ 25 , 34 , 57 , 99 ].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, AtNRT2.2 is only expressed in a compensatory manner when the function of NRT2.1 is lost [ 11 , 23 ]. AtNRT2.4 and AtNRT2.5 are expressed in the absence of N although AtNRT2.4 also participates in the loading of NO 3 − towards the phloem and AtNRT2.5 in the absorption and remobilization process in adult plants subjected to severe N deficiency [ 11 , 25 , 26 ]. Unlike these four transporters, AtNRT2.7 is found in the tonoplast, and this subcellular localization may explain its lack of interaction with NAR2.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

Zoghbi-Rodríguez

Gamboa-Tuz

Pereira-Santana

et al. 2021

IJMS

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Nitrate transporter 2 (NRT2) and NRT3 or nitrate-assimilation-related 2 (NAR2) proteins families form a two-component, high-affinity nitrate transport system, which is essential for the acquisition of nitrate from soils with low N availability. An extensive phylogenomic analysis across land plants for these families has not been performed. In this study, we performed a microsynteny and orthology analysis on the NRT2 and NRT3 genes families across 132 plants (Sensu lato) to decipher their evolutionary history. We identified significant differences in the number of sequences per taxonomic group and different genomic contexts within the NRT2 family that might have contributed to N acquisition by the plants. We hypothesized that the greater losses of NRT2 sequences correlate with specialized ecological adaptations, such as aquatic, epiphytic, and carnivory lifestyles. We also detected expansion on the NRT2 family in specific lineages that could be a source of key innovations for colonizing contrasting niches in N availability. Microsyntenic analysis on NRT3 family showed a deep conservation on land plants, suggesting a high evolutionary constraint to preserve their function. Our study provides novel information that could be used as guide for functional characterization of these gene families across plant lineages.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

Zoghbi-Rodríguez

Gamboa-Tuz

Pereira-Santana

et al. 2021

IJMS

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“…In plants, ammonium nitrogen absorption is mainly regulated by the ammonium transporter (AMT) genes ( Li et al, 2017 ). Conversely, nitrate nitrogen uptake is mainly regulated by four types of NO 3 − transporters: low affinity nitrate transporter NRT1, high affinity nitrate transporter NRT2, chloride channel protein CLC, and slow anion channel related homologue SLAC1/SLAH ( Liu R. et al, 2020 ). Of these, the chloride channel (CLC) gene family is widely distributed across a variety of archaea, microbial fungi, mammals, and plants ( Park et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Functional Characterization of the Chloride Channel TaCLC Gene Family in Wheat (Triticum aestivum L.)

et al. 2022

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In plants, chloride channels (CLC) are involved in a series of specific functions, such as regulation of nutrient transport and stress tolerance. Members of the wheat Triticum aestivum L. CLC (TaCLC) gene family have been proposed to encode anion channels/transporters that may be related to nitrogen transportation. To better understand their roles, TaCLC family was screened and 23 TaCLC gene sequences were identified using a Hidden Markov Model in conjunction with wheat genome database. Gene structure, chromosome location, conserved motif, and expression pattern of the resulting family members were then analyzed. Phylogenetic analysis showed that the TaCLC family can be divided into two subclasses (I and II) and seven clusters (-a, -c1, -c2, -e, -f1, -f2, and -g2). Using a wheat RNA-seq database, the expression pattern of TaCLC family members was determined to be an inducible expression type. In addition, seven genes from seven different clusters were selected for quantitative real-time PCR (qRT-PCR) analysis under low nitrogen stress or salt stress conditions, respectively. The results indicated that the gene expression levels of this family were up-regulated under low nitrogen stress and salt stress, except the genes of TaCLC-c2 cluster which were from subfamily -c. The yeast complementary experiments illustrated that TaCLC-a-6AS-1, TaCLC-c1-3AS, and TaCLC-e-3AL all had anion transport functions for NO3− or Cl−, and compensated the hypersensitivity of yeast GEF1 mutant strain YJR040w (Δgef1) in restoring anion-sensitive phenotype. This study establishes a theoretical foundation for further functional characterization of TaCLC genes and provides an initial reference for better understanding nitrate nitrogen transportation in wheat.

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Ion transporters and their exploration for conferring abiotic stress tolerance in plants

Tan

Shang

et al. 2021

Plant Growth Regul

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The expression patterns and putative function of nitrate transporter 2.5 in plants

Cited by 17 publications

References 78 publications

Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

Genome-Wide Identification and Functional Characterization of the Chloride Channel TaCLC Gene Family in Wheat (Triticum aestivum L.)

Ion transporters and their exploration for conferring abiotic stress tolerance in plants

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