The expression pattern and potential functions of PHB in the spermiogenesis of Phascolosoma esculenta

Hou, Cong-Cong; Gao, Xinming; Ni, Jie; Mu, Dan-Li; Yang, Haiyan; Liu, Cheng; Zhu, Junquan

doi:10.1016/j.gene.2018.01.056

Cited by 9 publications

(5 citation statements)

References 54 publications

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“…Regarding mitochondrial degradation, PHB proteins have been found in a variety of systems to be poly-ubiquitinated during spermiogenesis, suggesting a possible role in elimination of paternal mitochondria [110,111,112]. Recently, PHB2 has been reported as a mitochondrial receptor essential for Parkin mediated mitophagy, in mammalian cells.…”

Section: Mitochondrial Prohibitins Key Players In Mitochondrial Qmentioning

confidence: 99%

Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex

Hernando‐Rodríguez

Artal‐Sanz

2018

Cells

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Mitochondrial functions are essential for life, critical for development, maintenance of stem cells, adaptation to physiological changes, responses to stress, and aging. The complexity of mitochondrial biogenesis requires coordinated nuclear and mitochondrial gene expression, owing to the need of stoichiometrically assemble the oxidative phosphorylation (OXPHOS) system for ATP production. It requires, in addition, the import of a large number of proteins from the cytosol to keep optimal mitochondrial function and metabolism. Moreover, mitochondria require lipid supply for membrane biogenesis, while it is itself essential for the synthesis of membrane lipids. To achieve mitochondrial homeostasis, multiple mechanisms of quality control have evolved to ensure that mitochondrial function meets cell, tissue, and organismal demands. Herein, we give an overview of mitochondrial mechanisms that are activated in response to stress, including mitochondrial dynamics, mitophagy and the mitochondrial unfolded protein response (UPRmt). We then discuss the role of these stress responses in aging, with particular focus on Caenorhabditis elegans. Finally, we review observations that point to the mitochondrial prohibitin (PHB) complex as a key player in mitochondrial homeostasis, being essential for mitochondrial biogenesis and degradation, and responding to mitochondrial stress. Understanding how mitochondria responds to stress and how such responses are regulated is pivotal to combat aging and disease.

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Section: Mitochondrial Prohibitins Key Players In Mitochondrial Qmentioning

confidence: 99%

Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex

Hernando‐Rodríguez

Artal‐Sanz

2018

Cells

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“…Regarding mitochondrial degradation, PHB proteins have been found in a variety of systems to be poly-ubiquitinated during spermiogenesis, suggesting a possible role in elimination of paternal mitochondria [109][110][111]. Recently, PHB2 has been reported as a mitochondrial receptor essential for Parkin mediated mitophagy, in mammalian cells.…”

Section: The Phb Complex In Mitochondrial Turnovermentioning

confidence: 99%

Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex

Hernando‐Rodríguez¹,

Artal‐Sanz²

2018

Preprint

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Mitochondrial functions are essential for life, critical for development, maintenance of stem cells, adaptation to physiological changes, responses to stress and aging. The complexity of mitochondrial biogenesis requires coordinated nuclear and mitochondrial gene expression, owing to the need of stoichiometrically assemble the OXPHOS system for ATP production. It requires, in addition, the import of thousands of proteins from the cytosol to keep optimal mitochondrial function and metabolism. Moreover, mitochondria require lipid supply for membrane biogenesis, while it is itself essential for the synthesis of membrane lipids. To achieve mitochondrial homeostasis, multiple mechanisms of quality control have evolved to ensure that mitochondrial function meets cell, tissue and organismal demands. Herein, we give an overview of mitochondrial mechanisms that are activated in response to stress, including mitochondrial dynamics, mitophagy and the mitochondrial unfolded protein response (UPRmt). We then discuss the role of these stress responses in aging, with particular focus on Caenorhabditis elegans. Finally, we review observations that point to the mitochondrial PHB (prohibitin) complex as a key player in mitochondrial homeostasis, being essential for mitochondrial biogenesis and degradation, and responding to mitochondrial stress. Understanding how mitochondria responds to stress and how such responses are regulated is pivotal to combat aging and disease.

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“…During spermiogenesis, mitochondria undergo ubiquitination modifications to degrade damaged or excess mitochondria, as observed in Bos taurus , Eriocheir sinensis , Procambarus clarkia , and Phascolosoma esculenta , where the colocalization of mitochondrial and polyubiquitin signals has been detected in early to mature spermatids [ 22 , 29 , 43 , 44 ]. PHB1 is a substrate for sperm mitochondrial ubiquitination [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…The primers that were used are listed in Table 1 . Various online tools were utilized to predict the protein structure of PHBs, employing previously established methods [ 22 ] as outlined in Table 2 . The amino acid sequence of Ot -PHB1 and Ot -PHB2 proteins were aligned with their homologs from other species using Vector NTI10 software.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Prohibitins on Mitochondrial Function during Octopus tankahkeei Spermiogenesis

Wang

Gao

et al. 2023

IJMS

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Mitochondria are essential for spermiogenesis. Prohibitins (PHBs; prohibitin 1, PHB1 or PHB, and prohibitin 2, PHB2) are evolutionarily conserved and ubiquitously expressed mitochondrial proteins that act as scaffolds in the inner mitochondrial membrane. In this study, we analyzed the molecular structure and dynamic expression characteristics of Ot-PHBs, observed the colocalization of Ot-PHB1 with mitochondria and polyubiquitin, and studied the effect of phb1 knockdown on mitochondrial DNA (mtDNA) content, reactive oxygen species (ROS) levels, and apoptosis-related gene expression in spermatids. Our aim was to explore the effect of Ot-PHBs on mitochondrial function during the spermiogenesis of Octopus tankahkeei (O. tankahkeei), an economically important species in China. The predicted Ot-PHB1/PHB2 proteins contained an N-terminal transmembrane, a stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (also known as the prohibitin domain), and a C-terminal coiled-coil domain. Ot-phb1/phb2 mRNA were widely expressed in the different tissues, with elevated expression in the testis. Further, Ot-PHB1 and Ot-PHB2 were highly colocalized, suggesting that they may function primarily as an Ot-PHB compiex in O. tankahkeei. Ot-PHB1 proteins were mainly expressed and localized in mitochondria during spermiogenesis, implying that their function may be localized to the mitochondria. In addition, Ot-PHB1 was colocalized with polyubiquitin during spermiogenesis, suggesting that it may be a polyubiquitin substrate that regulates mitochondrial ubiquitination during spermiogenesis to ensure mitochondrial quality. To further investigate the effect of Ot-PHBs on mitochondrial function, we knocked down Ot-phb1 and observed a decrease in mtDNA content, along with increases in ROS levels and the expressions of mitochondria-induced apoptosis-related genes bax, bcl2, and caspase-3 mRNA. These findings indicate that PHBs might influence mitochondrial function by maintaining mtDNA content and stabilizing ROS levels; in addition, PHBs might affect spermatocyte survival by regulating mitochondria-induced apoptosis during spermiogenesis in O. tankahkeei.

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The expression pattern and potential functions of PHB in the spermiogenesis of Phascolosoma esculenta

Cited by 9 publications

References 54 publications

Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex

Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex

Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex

The Effect of Prohibitins on Mitochondrial Function during Octopus tankahkeei Spermiogenesis

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