The expression of two novel orange-spotted grouper (Epinephelus coioides) TNF genes in peripheral blood leukocytes, various organs, and fish larvae

Lam, Freda Wai San; Wu, Szu Yin; Lin, Shih-Jie; Lin, Chin Chou; Chen, Yi Ming; Wang, Han Ching; Chen, Tzong Yueh; Han, Lin; Lin, John Han You

doi:10.1016/j.fsi.2010.12.011

Cited by 93 publications

(34 citation statements)

References 37 publications

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“…Existing procedures were followed to purify PBLs from blood samples (Lam et al, 2011). Headkidney-derived MO/MФ cells were also isolated and cultured as previously described (Zhang, Shi, & Chen, 2015).…”

Section: Cell Preparationsmentioning

confidence: 99%

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Li¹,

Ding²,

Chen³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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The MHC (major histocompatibility complex) class II family plays a major role in vertebrate immune systems and is normally found only on antigen-presenting cells. In this study, we identified the gene encoding the α (PaMHCIIα) chain of MHC class II molecules in ayu (Plecoglossus altivelis). Phylogenetic analysis showed that PaMHCIIα was tightly grouped with MHC class IIα in rainbow smelt (Osmerus mordax) and salmon. PaMHCIIα transcripts were detected in all tested tissues, with high levels in the spleen, head kidney, and gill; expression increased significantly upon Vibrio anguillarum infection. PaMHCIIα mRNA expression was also up-regulated in monocytes/macrophages (MO/MФ) after in vitro stimulation with V. anguillarum. We prepared antibodies for detecting N-glycosylation of PaMHCIIα or neutralizing PaMHCIIα on ayu MO/MФ. Western blot analysis showed that native PaMHCIIα was indeed N-glycosylated. Moreover, PaMHCIIα neutralization not only significantly inhibited ayu MO/MФ phagocytosis, but also reduced the ability of V. anguillarum to induce MO/MФ-cytokine expression. Overall, these findings reveal that the important role of PaMHCIIα against bacterial challenge, and it likely contributes to pathogen responsiveness in ayu MO/MФ.

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Section: Cell Preparationsmentioning

confidence: 99%

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Li¹,

Ding²,

Chen³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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“…Multiple isoforms of TNF-a genes have been identified in some fish species [e.g., three TNF-a genes are known in common carp (18,27) and two are known in rainbow trout (28), blue fin tuna Thunnus orientalis (29), and orange-spotted grouper Epinephelus coioides (30)]. The homology between the different isoforms within common carp and rainbow trout (i.e., in the same species) is high: ∼88% identity between the two trout isoforms and 72-78% identity among the three carp TNF molecules (27).…”

mentioning

confidence: 99%

“…Thus, they may have arisen from the recent tetraploidization of the ancestors of trout and carp (27). However, the homology of the TNF-a isoforms identified in blue fin tuna (29) and orange-spotted grouper (30) are much lower, with identities of 43 and 38%, respectively. These isoforms of TNF-a might have arisen from the teleost-wide whole-genome duplication (WGD) event (31), as seen with other fish cytokines, such as M-CSF (32) and IL-1b (33), for which two major forms exist in fish.…”

mentioning

confidence: 99%

“…These isoforms of TNF-a might have arisen from the teleost-wide whole-genome duplication (WGD) event (31), as seen with other fish cytokines, such as M-CSF (32) and IL-1b (33), for which two major forms exist in fish. However, the origin of the tuna and grouper isoforms is unclear, because phylogenetic tree analysis did not support the notion that two types of teleost TNF-a genes may exist as a result of the teleost-wide WGD (29,30).…”

mentioning

confidence: 99%

“…For example, in grouper, one isoform is highly expressed constitutively but is poorly upregulated by immune challenge in vitro and in vivo (29,30). In tuna, grouper, and trout, the TNF-a paralogues are differentially expressed and modulated (28)(29)(30), perhaps suggesting a degree of subfunctionalization (34). The differential expression of the paralogues could allow a fine-tuned regulation of TNF-a-induced cytokines in these species.…”

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confidence: 99%

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Two Types of TNF-α Exist in Teleost Fish: Phylogeny, Expression, and Bioactivity Analysis of Type-II TNF-α3 in Rainbow Trout Oncorhynchus mykiss

Hong

et al. 2013

The Journal of Immunology

184

160

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TNF-α is a cytokine involved in systemic inflammation and regulation of immune cells. It is produced chiefly by activated macrophages as a membrane or secreted form. In rainbow trout, two TNF-α molecules were described previously. In this article, we report a third TNF-α (TNF-α3) that has only low identities to known trout molecules. Phylogenetic tree and synteny analyses of trout and other fish species suggest that two types (named I and II) of TNF-α exist in teleost fish. The fish type-II TNF-α has a short stalk that may impact on its enzymatic release or restrict it to a membrane-bound form. The constitutive expression of trout TNF-α3 was generally lower than the other two genes in tissues and cell lines, with the exception of the macrophage RTS-11 cell line, in which expression was higher. Expression of all three TNF-α isoforms could be modulated by crude LPS, peptidoglycan, polyinosinic:polycytidylic acid, and rIFN-γ in cell lines and primary macrophages, as well as by bacterial and viral infections. TNF-α3 is the most responsive gene at early time points post-LPS stimulation and can be highly induced by the T cell–stimulant PHA, suggesting it is a particularly important TNF-α isoform. rTNF-α3 produced in CHO cells was bioactive in different cell lines and primary macrophages. In the latter, it induced the expression of proinflammatory cytokines (IL-1β, IL-6, IL-8, IL-17C, and TNF-αs), negative regulators (SOCS1–3, TGF-β1b), antimicrobial peptides (cathelicidin-1 and hepcidin), and the macrophage growth factor IL-34, verifying its key role in the inflammatory cytokine network and macrophage biology of fish.

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Humoral and Cellular Immune Response of European Seabass Dicentrarchus labrax Vaccinated with Heat‐Killed Mycobacterium marinum (iipA::kan Mutant)

et al. 2018

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No vaccine is yet commercially available against Mycobacterium marinum, the etiological agent of fish mycobacteriosis (also known as "fish tuberculosis"). The mycobacterial gene responsible for invasion and intracellular persistence, iipA, is known to moderate M. marinum pathology in Zebrafish Danio rerio. Two doses of heat-killed, wild-type, virulent M. marinum and two doses of a heat-killed, avirulent M. marinum iipA::kan mutant strain were used in parallel to vaccinate European Seabass Dicentrarchus labrax. The fish were then challenged with live, virulent M. marinum, and the pathogenesis of the infection was monitored. High specific immunoglobulin M (IgM) response and an increase in cytokine tumor necrosis factor alpha (TNF-α) messenger RNA expression levels were observed in all vaccinated fish. At 1 month postchallenge, TNF-α expression levels increased in spleen tissues of fish vaccinated with the virulent type and in those of unvaccinated fish, whereas in the head kidney, expression was up-regulated only in unvaccinated fish. The expression then decreased, and at 2 months postchallenge, expression appeared similar in all vaccination types. The highest survival rate (75%) was recorded in the group of fish that were vaccinated with a high dose of avirulent iipA::kan mutant. The iipA::kan mutant induced a strong immune response accompanied by only modest tissue disruption. Coupled with an effective program of booster treatments, the iipA::kan mutant vaccine may be developed into a powerful preventive measure against fish mycobacteriosis.

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The expression of two novel orange-spotted grouper (Epinephelus coioides) TNF genes in peripheral blood leukocytes, various organs, and fish larvae

Cited by 93 publications

References 37 publications

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Two Types of TNF-α Exist in Teleost Fish: Phylogeny, Expression, and Bioactivity Analysis of Type-II TNF-α3 in Rainbow Trout Oncorhynchus mykiss

Humoral and Cellular Immune Response of European Seabass Dicentrarchus labrax Vaccinated with Heat‐Killed Mycobacterium marinum (iipA::kan Mutant)

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