The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas

Reuß, Reinhard; Aberle, Susanne; Klingel, Karin; Sauter, Martina; Greschniok, A; Franke, Folker E.; Padberg, Winfried; Blin, Nikolaus

doi:10.3892/ijo.29.3.649

Cited by 8 publications

(9 citation statements)

References 12 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Excluding lymph nodes, the distribution of BCL10 immunostaining, identified with the monoclonal (331.3) antibody, among normal and ectopic pancreas, acinar metaplasia, and ACCs well reflected that of CEH. Our results on CEH distribution (Table 2) are in line with those previously published, since CEH was found in the pancreas and lactating breast, while neither CEH protein nor mRNA were observed in other normal tissues, with the exception of the gastric mucosa [9,10,[12][13][14][27][28][29].…”

Section: Discussionsupporting

confidence: 94%

See 1 more Smart Citation

The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia

Rosa¹,

Franzi

Marchet

et al. 2008

Virchows Arch

View full text Add to dashboard Cite

Acinar cell carcinoma (ACC) is a rare pancreatic cancer which may be difficult to distinguish from other solid nonadenocarcinoma tumors. The diagnosis depends on the demonstration of acinar differentiation, obtained with antibodies recognizing various pancreatic enzymes that, although specific, show different sensitivity. The C-terminal portion of the BCL10 protein shows homology with carboxyl ester hydrolase (CEH), an enzyme produced by pancreatic acinar cells. We investigated the usefulness of a C-terminal BCL10 monoclonal antibody in the diagnosis of ACCs. We examined normal pancreases and different pancreatic tumors including ACCs, mixed acinar-endocrine carcinomas, ductal adenocarcinomas, mucinous, serous, solid pseudopapillary, and endocrine neoplasms. In addition, various normal tissues and cases of pancreatic metaplasia of the gastroesophageal mucosa, cases of ectopic pancreas, gastrointestinal endocrine tumors, salivary and breast acinic cell carcinomas, gastric adenocarcinomas with and without acinar differentiation, and hepatocellular carcinomas were studied. BCL10 immunoreactivity paralleled that of CEH and was restricted to acinar cells of normal and ectopic pancreas, of pancreatic metaplasia, and of ACCs. The anti-BCL10 antibody was more sensitive in detecting ACCs and pancreatic metaplasia than antibodies directed against other pancreatic enzymes. We suggest using BCL10 antibody for diagnosing pancreatic tumors and whenever an acinar differentiation is suspected in gastrointestinal neoplastic and metaplastic lesions.

show abstract

Section: Discussionsupporting

confidence: 94%

“…In addition, the fact that we did not find BCL10-IR in other pancreatic tumors confirms that BCL10 immunohistochemistry is highly specific in detecting pancreatic acinar cell differentiation. Moreover, the lack of CEH expression in pancreatic tumors other than ACCs is in agreement with the findings reported by Reuss et al [27].…”

Section: Discussionsupporting

confidence: 90%

The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia

Rosa¹,

Franzi

Marchet

et al. 2008

Virchows Arch

View full text Add to dashboard Cite

show abstract

“…We observed that CEL expression disappeared when acinar-toductal metaplasia was present, confirming that the protein is a marker of pancreatic acinar cells only when these remain polarized and in acini-like arrangement. Our results are consistent with those of Reuss and coworkers (39). When they examined 25 ductal or mucinous adenocarcinomas from the pancreas, all cases were negative for CEL mRNA by in situ hybridization.…”

Section: Discussionsupporting

confidence: 93%

The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants

Jellas

Johansson

Fjeld

et al. 2018

Journal of Biological Chemistry

View full text Add to dashboard Cite

Carboxyl-ester lipase (CEL) is a pancreatic fat-digesting enzyme associated with human disease. Rare mutations in the CEL gene cause a syndrome of pancreatic exocrine and endocrine dysfunction denoted MODY8, whereas a recombined CEL allele increases the risk for chronic pancreatitis. Moreover, CEL has been linked to pancreatic ductal adenocarcinoma (PDAC) through a postulated oncofetal CEL variant termed feto-acinar pancreatic protein (FAPP). The monoclonal antibody mAb16D10 was previously reported to detect a glycotope in the highly O-glycosylated, mucin-like C terminus of CEL/FAPP. We here assessed the expression of human CEL in malignant pancreatic lesions and cell lines. CEL was not detectably expressed in neoplastic cells, implying that FAPP is unlikely to be a glycoisoform of CEL in pancreatic cancer. Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal GalNAc-␣1,3(Fuc-␣1,2)Gal (blood group A antigen) and also repeated protein sequences containing GalNAc residues linked to Ser/Thr (Tn antigen), findings that were supported by immunostainings of human pancreatic tissue. To examine whether the CEL glycoprotein might be modified by blood group antigens, we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool of CEL immunoprecipitated from human pancreatic juice. We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subject's FUT2 and ABO gene polymorphisms. Thus, among digestive enzymes secreted by the pancreas, CEL is a glycoprotein with some unique characteristics, supporting the view that it could serve additional biological functions to its cholesteryl esterase activity in the duodenum.

show abstract

“…CEH is a glycoprotein secreted by pancreatic acinar cells [10] and lactating mammary gland [11] involved in cholesterol and lipid-soluble vitamin ester hydrolysis and absorption. It has been previously observed in pancreatic acinar cell carcinomas [12,13] but not in pancreatic ductal adenocarcinomas. As recently demonstrated by La Rosa et al [14], its presence in tumour cells may be proved immunohistochemically using a specific anti-CEH antibody as well as an anti-C-terminal BCL10 monoclonal antibody, because the C-terminal extremities of both CEH and BCL10 protein share the same amino acidic sequence.…”

Section: Resultsmentioning

confidence: 85%

Colonic carcinoma with a pancreatic acinar cell differentiation. A case report

Chiaravalli¹,

Finzi²,

Bertolini

et al. 2009

Virchows Arch

View full text Add to dashboard Cite

A case of a colonic carcinoma showing a pancreatic acinar cell differentiation is described for the first time. A 65-year-old woman underwent surgical resection for an ulcerated protruding tumour of 4 x 2.5 cm in size on the anterior wall of the sigmoid colon. Histologically, tumour cells were organized in acinar structures resembling pancreatic acini and in solid nests and ribbons or diffusely infiltrated as poorly cohesive cells. Lymph nodes and femur metastases displayed the same histological features. The ultrastructural analysis of the primary tumour indicated the presence of zymogen-like granules in the cytoplasm of tumour cells. Immunohistochemically, both acinar and diffuse patterns of growth showed an intense staining for trypsin, chymotrypsin and BCL10 and a weaker immunoreactivity for lipase and carboxyl ester hydrolase. Most tumour cells were cytokeratin 20, CDX2 and p53 positive; whereas, mucin (MUC)2 immunoreactivity was observed only in the signet ring cells present in the diffuse pattern and chromogranin A in rare isolated tumour cells. No immunoreactivity was observed for cytokeratin 7, MUC1, MUC5AC, pancreatic amylase or PDX1. There was no evidence of a pancreatic acinar cell carcinoma or of heterotopic pancreatic tissue. A colonic origin ought to be suspected when a metastatic carcinoma of unknown primary shows an acinar differentiation.

show abstract

The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas

Cited by 8 publications

References 12 publications

The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia

The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia

The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants

Colonic carcinoma with a pancreatic acinar cell differentiation. A case report

Contact Info

Product

Resources

About