The Expression of Superoxide Dismutase (SOD) and a Putative ABC Transporter Permease Is Inversely Correlated during Biofilm Formation in Listeria monocytogenes 4b G

Suo, Yujuan; Liu, Yanhong; Shi, Chunlei; Shi, Xianming

doi:10.1371/journal.pone.0048467

Cited by 41 publications

(31 citation statements)

References 38 publications

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“…L. monocytogenes has two ABC transporters with significant sequence homology to these proteins: AnrAB and the putative transporter encoded by lmo1746-lmo1747. Previous studies have identified lm.G_1771, an ABC transporter permease in L. monocytogenes serotype 4b strain G that is identical to lmo1746, and found that lm.G_1771 had a role in the regulation of biofilm formation (27,28). Further microarray analyses indicated that the product of lm.G_1771 regulates the transcription of anrAB and dltABCD, members of the VirR regulon (29).…”

Section: Resultsmentioning

confidence: 99%

The VirAB ABC Transporter Is Required for VirR Regulation of Listeria monocytogenes Virulence and Resistance to Nisin

et al. 2018

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is a Gram-positive intracellular pathogen that causes a severe invasive disease. Upon infecting a host cell, upregulates the transcription of numerous factors necessary for productive infection. VirR is the response regulator component of a two-component regulatory system in In this report, we have identified the putative ABC transporter encoded by genes as necessary for VirR function. We have designated We constructed an in-frame deletion of and determined that the Δ mutant exhibited reduced transcription of VirR-regulated genes. The Δ mutant also showed defects in plaque formation and virulence that were similar to those of a Δ deletion mutant. Since VirR is important for innate resistance to antimicrobial agents, we determined the MICs of nisin and bacitracin for Δ bacteria. We found that VirAB expression was necessary for nisin resistance but was dispensable for resistance to bacitracin. This result suggested a VirAB-independent mechanism of VirR regulation in response to bacitracin. Lastly, we found that the Δ and Δ mutants had no deficiency in growth in broth culture, intracellular replication, or production of the ActA surface protein, which facilitates actin-based motility and cell-to-cell spread. However, the Δ and Δ mutants produced shorter actin tails during intracellular infection, which suggested that these mutants have a reduced ability to move and spread via actin-based motility. These findings have demonstrated that VirAB functions in a pathway with VirR to regulate the expression of genes necessary for virulence and resistance to antimicrobial agents.

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Section: Resultsmentioning

confidence: 99%

The VirAB ABC Transporter Is Required for VirR Regulation of Listeria monocytogenes Virulence and Resistance to Nisin

et al. 2018

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“…It has been reported that ROS is associated with the reduction in the amount of bio film formation (Suo et al, 2012). It was also suggested that the formation of bio films and the secretion of extracellular polysaccharides are also part of the ROS defense strategies of L. ferriphilum (Bellenberg et al, 2014).…”

Section: Bio Film Under the Condition Of Stress By LI Ion And Cobalt Ionmentioning

confidence: 99%

Oxidative Stress Induced by Metal Ions in Bioleaching of LiCoO2 by an Acidophilic Microbial Consortium

Liu

Líu

et al. 2020

Front. Microbiol.

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An acidophilic microbial consortium (AMC) was used to investigate the fundamental mechanism behind the adverse effects of pulp density increase in the bioleaching of waste lithium ion batteries (WLIBs). Results showed that there existed the effect of metal-ion stress on the bio-oxidative activity of AMC. The Li + and Co 2+ accumulated in the leachate were the direct cause for the decrease in lithium and cobalt recovery yields under a high pulp density. In a simulated bioleaching system with 4.0% (w •v −1) LiCoO 2 , the intracellular reactive oxygen species (ROS) content in AMC increased from 0.82 to 6.02 within 24 h, which was almost three times higher than that of the control (2.04). After the supplementation of 0.30 g•L −1 of exogenous glutathione (GSH), the bacterial intracellular ROS content decreased by 40% within 24 h and the activities of intracellular ROS scavenging enzymes, including glutathione peroxidase (GSH-Px) and catalase (CAT), were 1.4-and 2.0-folds higher in comparison with the control within 24 h. In the biofilms formed on pyrite in the bioleaching of WLIBs, it was found that metal-ion stress had a great influence on the 3-D structure and the amount of biomass of the biofilms. After the exogenous addition of GSH, the structure and the amount of biomass of the biofilms were restored to some extent. Eventually, through ROS regulation by the exogenous addition of GSH, very high metal recovery yields of 98.1% Li and 96.3% Co were obtained at 5.0% pulp density.

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“…A study that observed filaments in biofilms formed by certain strains of L. monocytogenes argued that these filaments were probably flagella, because they were not observed in nonmotile strains of L. monocytogenes (111). Some studies (98,112) suggest that B plays a role in establishing Listeria attachment communities. For example, van der Veen and Abee (98) showed differential sigB expression in L. monocytogenes strain EGD-e during growth on surfaces.…”

Section: B In Biofilmsmentioning

confidence: 99%

Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria

Guldimann

Boor

Wiedmann

et al. 2016

Appl Environ Microbiol

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Gram-positive bacteria are ubiquitous and diverse microorganisms that can survive and sometimes even thrive in continuously changing environments. The key to such resilience is the ability of members of a population to respond and adjust to dynamic conditions in the environment. In bacteria, such responses and adjustments are mediated, at least in part, through appropriate changes in the bacterial transcriptome in response to the conditions encountered. Resilience is important for bacterial survival in diverse, complex, and rapidly changing environments and requires coordinated networks that integrate individual, mechanistic responses to environmental cues to enable overall metabolic homeostasis. In many Gram-positive bacteria, a key transcriptional regulator of the response to changing environmental conditions is the alternative sigma factor B . B has been characterized in a subset of Gram-positive bacteria, including the genera Bacillus, Listeria, and Staphylococcus. Recent insight from nextgeneration-sequencing results indicates that B -dependent regulation of gene expression contributes to resilience, i.e., the coordination of complex networks responsive to environmental changes. This review explores contributions of B to resilience in Bacillus, Listeria, and Staphylococcus and illustrates recently described regulatory functions of B .

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The Expression of Superoxide Dismutase (SOD) and a Putative ABC Transporter Permease Is Inversely Correlated during Biofilm Formation in Listeria monocytogenes 4b G

Cited by 41 publications

References 38 publications

The VirAB ABC Transporter Is Required for VirR Regulation of Listeria monocytogenes Virulence and Resistance to Nisin

The VirAB ABC Transporter Is Required for VirR Regulation of Listeria monocytogenes Virulence and Resistance to Nisin

Oxidative Stress Induced by Metal Ions in Bioleaching of LiCoO2 by an Acidophilic Microbial Consortium

Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria

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