The expression of Sirtuins 1 and 4 in peripheral blood leukocytes from patients with type 2 diabetes

Abstract

This study was designed to investigate the relationship between SIRTs 1 and 4 in peripheral blood leukocytes (PBLs) and human type 2 diabetes mellitus (T2DM). SIRTs 1 and 4 have been confirmed to be associated with homeostasis of glucose/lipid metabolism, but their roles in T2DM are still poorly understood. Peripheral blood and biochemical data were collected from 52 healthy individuals (normal control group, NC group) and 113 cases of T2DM patients. Immunocytochemical staining was used to detect SIRT1 and SIRT4 and reverse transcription polymerase chain reaction (RT-PCR) was used to detect SIRT1 or SIRT4 mRNA levels in PBLs. Immunocytochemical staining showed that SIRT1 is expressed in both nucleus and cytoplasm, and SIRT4 in the cytoplasm of granulocytes and monocytes. No SIRT1 or SIRT4 was found in lymphocytes. RT-PCR showed that SIRT1 and SIRT4 mRNA levels in T2DM group were lower than those in NC group (P<0.01). Correlation analysis showed that there is a negative correlation between SIRTs 1 and 4 and fasting plasma glucose (FPG) (P<0.05) (r= −0.161 and −0.156), a positive correlation between SIRT4 mRNA levels and triglyceride (TG)/lipoprotein a (LPa) levels (P<0.05), and a negative correlation between SIRT4 mRNA levels and high density lipoprotein cholesterol (HDL) (P<0.05). SIRTs 1 and 4 may have a role in the pathogenesis of T2DM and their expression in granulocytes and monocytes may indirectly reflect the homeostasis of glucose/lipid metabolism in T2DM.

Sirt1 expression is associated with CD31 expression in blood cells from patients with chronic obstructive pulmonary disease

“…2d). Previous studies showed that Sirt1 expression in blood cells is involved in diseases associated with systemic chronic inflammatory processes such as coronary artery disease, type 2 diabetes [35, 36]. Moreover, Mody et al, reported that decreased Sirt1 expression in pulmonary leukocyte was associated with the development of bronchopulmonary dysplasia [37].…”

Preliminary data on the relationship between circulating levels of Sirtuin 4, anthropometric and metabolic parameters in obese subjects according to growth hormone/insulin-like growth factor-1 status

“…Aside from obesity, glucose metabolism is another factor which might influence Sirt4. Evidence has been provided that mRNA expression of Sirt4 in granulocytes and monocytes of T2DM patients was lower compared with healthy individuals, with a negative correlation between Sirt4 and fasting plasma glucose [12]. However, after adjusting for insulin, Sirt4 still remained significantly correlated with BMI, peak GH and IGF-1 levels.…”

“…Sirtuin 1 and Sirtuin 4 (Sirt4) are involved in insulin secretion [11] and in maintaining the balance of glucose/lipid metabolism in type 2 diabetes mellitus (T2DM) [12]. Sirt4, ubiquitously expressed in many organs including skeletal muscle and the liver, functions within the mitochondria as negative regulator of mitochondrial oxidative capacity [13].…”

Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 1

“…SIRT1 is a nuclear NAD + -dependent class III histone deacetylase that may regulate cell aging, life span and energy metabolism (13) by engaging in the reciprocal co-regulation of different binding partners (14). SIRT1 is able to adjust the deacetylase activity of various transcription factors that control metabolic and endocrine signals and is widely involved in regulating the mammalian cell life span, INS secretion and glucose/lipid metabolism (15). Genetic variation in the SIRT1 gene is associated with INS resistance and may explain the increased risk of T2DM in the Chinese Han population (16).…”