The expression of prostatic acid phosphatase is transcriptionally regulated in human prostate carcinoma cells

Garcia-Arenas, Renee; Lin, Fen Fen; Lin, Din-Lii; Jin, Li; Shih, Charles C.-Y.; Chang, Chawnshang; Lin, Ming‐Fong

doi:10.1016/0303-7207(95)03544-h

Cited by 33 publications

(28 citation statements)

References 23 publications

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“…Gene array study identified sGCa1 as a novel androgenregulated gene Working from the hypothesis that novel androgen-regulated genes involved in androgen-induced proliferation of the prostate cancer cells LNCaP could be identified using gene microarrays, we developed an experimental strategy that utilized PC-3 cells stably transfected with AR (called A103 cells) as a negative control, as these cells exhibit DHT-repressed proliferation (see Figure 2b; Yuan et al, 1993;Garcia-Arenas et al, 1995;Heisler et al, 1997). Therefore, a gene array analysis was carried out with C14 (LNCaP cells transfected with empty pCI-Neo vector) and A103 cells using the Affymetrix human genome chip Hu95A, leading to the identification of several known (e.g.…”

Section: Resultsmentioning

confidence: 99%

Androgen regulation of soluble guanylyl cyclaseα1 mediates prostate cancer cell proliferation

et al. 2006

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The growth and progression of prostate cancer are dependent on androgens and androgen receptor (AR), which act by modulating gene expression. Utilizing a gene microarray approach, we have identified the a1-subunit gene of soluble guanylyl cyclase (sGC) as a novel androgen-regulated gene. A heterodimeric cytoplasmic protein composed of one a and one b subunit, sGC mediates the widespread cellular effects of nitric oxide (NO). We report here that, in prostate cancer cells, androgens stimulate the expression of sGCa1. A cloned human sGCa1 promoter is activated by androgen in an AR-dependent manner, suggesting that sGCa1 may be a direct AR target gene. Disruption of sGCa1 expression severely compromises the growth of both androgendependent and androgen-independent AR-positive prostate cancer cells. Overexpression of sGCa1 alone is sufficient for stimulating prostate cancer cell proliferation. Interestingly, the major growth effect of sGCa1 is independent of NO and cyclic guanosine monophosphate, a major mediator of the sGC enzyme. These data strongly suggest that sGCa1 acts in prostate cancer via a novel pathway that does not depend on sGCb1. Tissue studies show that sGCa1 expression is significantly elevated in advanced prostate cancer. Thus, sGCa1 may be an important mediator of the procarcinogenic effects of androgens.

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Section: Resultsmentioning

confidence: 99%

Androgen regulation of soluble guanylyl cyclaseα1 mediates prostate cancer cell proliferation

et al. 2006

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“…Northern Blot Analysis-Total RNA was prepared from cells by a single step guanidine isothiocyanate-phenol-chloroform method (34,35). An aliquot of each total RNA sample was electrophoresed in a 1.2% agarose gel containing formaldehyde as a denaturing agent (22,35).…”

Section: Methodsmentioning

confidence: 99%

Expression of Human Prostatic Acid Phosphatase Correlates with Androgen-stimulated Cell Proliferation in Prostate Cancer Cell Lines

Lin¹,

Meng²,

Rao³

et al. 1998

Journal of Biological Chemistry

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124

164

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Androgen plays a critical role in regulating the growth and differentiation of normal prostate epithelia, as well as the initial growth of prostate cancer cells. Nevertheless, prostate carcinomas eventually become androgen-unresponsive, and the cancer is refractory to hormonal therapy. To gain insight into the mechanism involved in this hormone-refractory phenomenon, we have examined the potential role of the androgen receptor (AR) in that process. We have investigated the expression of AR and two prostate-specific androgen-responsive antigens, prostatic acid phosphatase (

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“…This pathway which is under stromal influence is apparently not operative in LNCaP. To date, virtually all studies on the regulation of PSA and PAP expression have been carried out with cultured LNCaP cells (25,26). Our experi- A total of 118,000 CD57 ϩ and 1.5 ϫ 10 6 CD57 Ϫ cells were collected by flow sorting on the basis of ␣CD57-PE fluorescence from specimen LFo.…”

Section: Figmentioning

confidence: 99%

Cell–cell interaction in prostate gene regulation and cytodifferentiation

Liu

True

LaTray

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

179

154

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The expression of prostatic acid phosphatase is transcriptionally regulated in human prostate carcinoma cells

Cited by 33 publications

References 23 publications

Androgen regulation of soluble guanylyl cyclaseα1 mediates prostate cancer cell proliferation

Androgen regulation of soluble guanylyl cyclaseα1 mediates prostate cancer cell proliferation

Expression of Human Prostatic Acid Phosphatase Correlates with Androgen-stimulated Cell Proliferation in Prostate Cancer Cell Lines

Cell–cell interaction in prostate gene regulation and cytodifferentiation

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