The expression of prokaryotic tRNA genes in frog oocytes

Bossi, Lionello; Ciampi, M S

doi:10.1093/nar/11.10.3207

Cited by 8 publications

(2 citation statements)

References 37 publications

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“…It is more difficult to change tRNA in expression systems. Genes for tRNA species have been introduced into heterologous cells to change tRNA content (Brinkman et al, 1989;Hoekema et al, 1987;Rojiani et al, 1990;Wilson and Roe, 1989), but expression of tRNA genes in heterologous systems may be different from homologous cells and must be determined (Bossi and Ciampi, 1983). It is possible to achieve overexpression of tRNA species to alter tRNA content (e.g., Wilson and Roe, 1989;Borel et al, 1993;Pak et al, 1994).…”

Section: Problems and Opportunitiesmentioning

confidence: 99%

Problems of Translating Heterologous Genes in Expression Systems: The Role of tRNA

Smith

1996

Biotechnology Progress

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tRNA can have large effects on the expression and overexpression of heterologous genes in microbial expression systems through reduced translation and errors in amino acid sequences of protein products. Examples are given of large effects on gene expression related to tRNA content and to tRNA base modifications, both of which differ in heterologous expression systems compared to the cells from which the genes originally came. tRNA should be of greater concern in the expression of heterologous genes.

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Section: Problems and Opportunitiesmentioning

confidence: 99%

Problems of Translating Heterologous Genes in Expression Systems: The Role of tRNA

Smith

1996

Biotechnology Progress

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“…Because of the similarity and comparable position of these sequences to the Pribnow box, it seems reasonable to suppose that transcription of the tRNAArg operon may also initiate at the same corresponding G residue. Since this work was completed, Bossi and Ciampi have cloned a corresponding tRNA operon from Salmonella typhimurium (2). Characterization of the clone indicates conservation of the gene sequences for the two species, although many sequence differences occur in the spacer regions.…”

Section: Discussionmentioning

confidence: 99%

Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs

Hsu¹,

Klee²,

Zagorski³

et al. 1984

J Bacteriol

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A plasmid containing a gene for the most abundant Escherichia coli leucine isoacceptor tRNA,tRNALeU (anticodon CAG) was isolated from the Clarke-Carbon bank of cloned E. coli DNA. The clone contains a 12.3-kilobase DNA insert which was mapped by F' DNA hybridization analysis to the region 82 to 89 min on the chromosome. The cloned tDNALeU corresponds to the minor of two chromosomal regions containing different amounts of DNA complementary to tRNAcAeu&. Sequencing of the tDNA region revealed it to contain a multimeric transcription unit consisting of four different tRNA genes. The genes are in the arrangement 5'-leader-tRNAc73-57 base pairs-tRNAgusG-20 base pairs-tRNALAeuG-42 base pairs-tRNAUGG-3'. Coordinate expression of the component tRNAs in vivo and the absence of intercistronic promoters indicated that all four tDNAs reside in the same operon. The tDNA sequence is bounded by a promoter element showing good agreement with the procaryotic consensus sequence and a GC-rich stem-loop element that corresponds to a rho-independent terminator. The promoter region contains a GC-rich sequence that agrees with a suggested consensus stringency control element and two domains possessing dyad symmetry which flank the Pribnow box and include the putative stringency control region.

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The hisR locus of Salmonella: Nucleotide sequence and expression

Bossi

1983

Molec Gen Genet

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In S. typhimurium, the hisR locus is defined by mutations causing reduced levels of the histidine transfer RNA. As a preliminary step in the analysis of the hisR mutants, a 972 bp DNA fragment containing the histidine tRNA gene from wild-type Salmonella was cloned and completely sequenced. This analysis revealed the existence of a tRNA gene cluster which, in addition to the tRNAHis gene, includes the genes for tRNALeu1, tRNAPro1 and a tentative tRNAArgCGG. All four tRNA genes are present as single copies and are separated by spacer sequences ranging from 20 to 53 bp in length. The gene cluster is efficiently transcribed in vitro by E. coli RNA polymerase and yields a transcript, approximately 480 nucleotides long, which contains all four tRNA sequences. This tetrameric precursor can be processed to 4S RNA in vitro with a wild-type Salmonella extract, but not with an extract prepared from a hisU (RNase P) mutant. Using portions of the tRNA gene cluster as specific hybridization probes, various processing intermediates were shown to accumulate in vivo in the hisU mutant. Most of these RNAs are monomeric precursors only a few nucleotides longer than the respective mature tRNA species.

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The expression of prokaryotic tRNA genes in frog oocytes

Cited by 8 publications

References 37 publications

Problems of Translating Heterologous Genes in Expression Systems: The Role of tRNA

Problems of Translating Heterologous Genes in Expression Systems: The Role of tRNA

Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs

The hisR locus of Salmonella: Nucleotide sequence and expression

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