The expression of p53 and hsp70 proteins after treatment with Annona muricata Linn leaf for activating apoptotic and lead to homeostasis program of Raji cells

Abstract

Purpose: Organic extracts of plant Annonaceae enhances apoptosis in animal cells and get the drives to reach a new homeostasis. The incidence rate of nasopharyngeal cancer in Indonesia is quite high. Protein 53Kd (p53) play a role in apoptosis process, being heat shock protein 70 (hsp70) play a role in homeostasis. The aim of this research is to identify the apoptotic effects of Annona muricata Linn leaf toward Raji cells by observing the p53 and hsp70 expression. Methods: Apoptotic assay was performed in 24 wells micro-culture plate. Raji cells were prepared as 2 × 10 4 cells in 100 ml RPMI media per well. Roswell Park Memorial Institute (RPMI) medium was created and solvent was controlled with Dimethyl Sulfoxide (DMSO) solvent 0.25. Apoptotic test was performed by calculating trypan-blue-dye exclution. The cells were then grown in micro-culture plate with media plus extract non-lethal concentration of partition and fractionation of Annona muricata Linn leaf. The sampling was performed for 24 hours. The number of living cells was calculated in each of these well and incubation time were determined. Immunohistochemical staining was done to identify the expression of p53 and hsp70. Results: The results showed that Raji cells treated with partition of Annona muricata Linn leaf in ethyl acetate solvent 133.00 % resulted in higher apoptosis. Another results showed that Raji cells treated with fractionation Annona muricata Linn leaf in ethyl acetate solvent 103.20 % resulted in higher apoptosis. The expression of p53 after treatment with fractionation Annona muricata Linn leaf was higher than before while hsp70 expression after treatment with fractionation Annona muricata Linn leaf was lower. Conclusion: The conclusion is the higher the dose of Annona muricata Linn the higher the p53 expression thereby activates apoptosis process The higher dose of Anonna muricata Linn also leads to lower hsp70 expression indicating stable homeostasis of Raji cells.

Cytotoxicity of Soursop Leaves (Annona muricata) against Cervical HeLa Cancer Cells

“…8 Another study reported that soursop leaves extracts were known to increase expression of p53 gene and lower hsp70 expression, resulted in activation of apoptosis process and stable homeostasis in Raji cells. 9 However, research about cytotoxicity of Annona muricata leaves originating from Indonesia against cervical cancer are still limited. Therefore, this research aimed to analyze cytotoxicity of A.muricata leaves against cervical cancer because it is the leading cause of cancer-related death.…”

Phytochemistry and Antioxidant Activity of Soursop (Annona Muricata) Leaves

“…M Pai et al found that soursop extract has antimicrobial and fungicidal properties when used against oral microbiota [9]. Another study conducted by Astirin et al reported that extract of A. muricata activates the apoptosis process due to an increase in p53 expression in a cancer environment [10]. Padma et al showed that ethanolic extract of A. muricata caused a reduction in lipid peroxidation and increased protective effects against oxidative stress induced by carbon tetrachloride.…”

Efektivitas Ekstrak Etanol Daun Sirsak (Annona muricata Linn) dalam Mencegah Displasia Dorsum Lingue Tikus Galur Sprague Dawley yang Diinduksi 7,12 Dimetilbenz(Α)Antrasena

“…Efek antikanker ekstrak etanolik daun sirsak diduga berkaitan dengan kandungan senyawa acetogenin dan flavonoid daun sirsak. Efek antikanker daun sirsak dapat terjadi melalui beberapa mekanisme di antaranya menghambat proliferasi, 15,23 menginduksi apoptosis, 23 meningkatkan ekspresi p53, 24 dan melalui aktivitas antioksidan. 13 Analisis statistik data hasil penelitian menunjukkan tidak terdapat perbedaan terjadinya displasia ringan yang bermakna antara kelompok tikus yang diinduksi DMBA dengan kelompok tikus yang diinduksi DMBA dan diberi ekstrak etanol daun sirsak 100 mg/kg BB dan 200 mg/kg BB.…”