The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella

Jin, Haixia; Yin, Huiming; Zhang, Caiyun; Jinrong, W

doi:10.1590/1806-9061-2020-1338

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Chicken coccidiosis is an enteric disease caused by Eimeria infection, leading to severe economic losses associated with immunosuppression and a high level of mortality in the poultry industry worldwide [297]. In fact, Eimeria tenella infection was shown to significantly increase the expression of NF-κB mRNA in chicken cecal tissue in vivo [269], and a similar increase in the expression level of NF-κB was observed in chicken intestinal epithelial cells in vitro after infection with E. tenella sporozoites [298].…”

Section: Eimeria Tenellamentioning

confidence: 89%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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Redox biology is a very quickly developing area of modern biological sciences, and roles of redox homeostasis in health and disease have recently received tremendous attention. There are a range of redox pairs in the cells/tissues responsible for redox homeostasis maintenance/regulation. In general, all redox elements are interconnected and regulated by various means, including antioxidant and vitagene networks. The redox status is responsible for maintenance of cell signaling and cell stress adaptation. Physiological roles of redox homeostasis maintenance in avian species, including poultry, have received limited attention and are poorly characterized. However, for the last 5 years, this topic attracted much attention, and a range of publications covered some related aspects. In fact, transcription factor Nrf2 was shown to be a master regulator of antioxidant defenses via activation of various vitagenes and other protective molecules to maintain redox homeostasis in cells/tissues. It was shown that Nrf2 is closely related to another transcription factor, namely, NF-κB, responsible for control of inflammation; however, its roles in poultry have not yet been characterized. Therefore, the aim of this review is to describe a current view on NF-κB functioning in poultry with a specific emphasis to its nutritional modulation under various stress conditions. In particular, on the one hand, it has been shown that, in many stress conditions in poultry, NF-κB activation can lead to increased synthesis of proinflammatory cytokines leading to systemic inflammation. On the other hand, there are a range of nutrients/supplements that can downregulate NF-κB and decrease the negative consequences of stress-related disturbances in redox homeostasis. In general, vitagene–NF-κB interactions in relation to redox balance homeostasis, immunity, and gut health in poultry production await further research.

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Section: Eimeria Tenellamentioning

confidence: 89%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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“…Eimeria Tenella challenged birds increased expression of NF-kB together with other pro-inflammatory cytokines in chickens [37]. Therefore, reduced IL1β and NF-kB1 expression with increased levels of Ocimum gratissimum in the diet of the experimental birds is expected to down-regulate the expression of NF-kB2 in this study, but the reverse was the case in the expression of NF-kB2 at 28 and 56 days.…”

Section: Discussionmentioning

confidence: 60%

Dietary Inclusion of Scent Leaf Meal (Ocimum Gratissimum) Affects Immune Genes Expression in Chicken Spleen at 28 and 56 Days

Sorhue¹,

Onainor²,

Moemeka³

et al. 2021

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This study was conducted to examine the effects of scent leaf meal (Ocimum gratissimum) on expression of inflammatory cytokines in the spleen of two chicken strains. A total of 150birds (75 of each strain) were randomly allotted into five dietary treatments at fifteen birds per treatment. Birds were fed diet containing varying levels of Ocimum gratissimum leaf meal. Treatment one (T1) had 0% OG, while treatment two (T2), treatment three (T3), treatment four (T4) and treatment five (T5) had 0.5% OG, 1.00% OG, 1.5% OG and 2% OG respectively. Feed and water was provided adlibitum throughout the feeding trial. Three birds were slaughtered from each treatment at day 28 and day 56, spleen samples were collected and stored using RNALater in a -20oc freezer prior to RNA extraction. Real-time qPCR was performed in 40cycles using the PowerUp SYBR Green reagent and analyzed with the 2-∆∆Ct method. Gene expression data were subjected to two-way analysis of variance. Strain effect was significantly different (P<0.05) at both time points. All the genes studied significantly differed (P<0.05) in their expression patterns at 28 and 56days of age. Increased inclusion rate of the test ingredients significantly (P<0.05) reduced IL1β and NF-KB1, while increasing IL10 and NF-KB2. Ocimim gratissimum leaf meal shows promise in the regulation of inflammation in chickens and can be used to efficiently replace antibiotics in broiler production.

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“…Results showed the ST NR infection reduced ileal morphology in the ileum on D 7 and 21, which would be associated with induced inflammation due to ST NR infection ( Wu et al, 2018 ). The mRNA expression of TLR2 (an activator of the innate immune system) and NFκB (proinflammatory transcription factor related to inflammation, immune response, and cell apoptosis) were reduced due to ST NR infection D 7 ( Jin et al, 2020 ). While a statistical significance was not achieved ( P = 0.123), supplementation of TA linearly reduced mRNA expression of NFκB in the ileum, which supports that TA has an anti-inflammatory function ( Huang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%