The expression of molecule CD28 and CD38 on CD4+/CD8+ T lymphocytes in thymus and spleen elicited by Schistosoma japonicum infection in mice model

Li, Na; Ji, Pengyu; Song, Liying; Lei, Junxia; Lv, Ziquan; Wu, Zhongdao; Shao, Xiaorong; Sun, Xi

doi:10.1007/s00436-015-4507-y

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…B. microti infection also resulted in a significant increase in the T cell population in B. microti infected and co-infected mice, as reflected by the increase in the percent of CD3+ cells, the CD8a+ cells and CD4+ T cells (Figure 3D). This increase is consistent with previous observations in other parasitic diseases (Sponaas et al, 2006; Abel et al, 2012; Li et al, 2015). Significant increase in the T cells during B. microti infection from ∼17% in naïve to ∼29% in B. microti infection ( p < 0.001, df = 6, F = 6.4) and ∼33% during co-infection ( p < 0.01, df = 6, F = 9.99) compared to ∼22% in N40 infected mice emphasizes the role of these cells specifically during early stage of B. microti infection.…”

Section: Resultssupporting

confidence: 93%

Protozoan Parasite Babesia microti Subverts Adaptive Immunity and Enhances Lyme Disease Severity

Djokić¹,

Akoolo²,

Primus³

et al. 2019

Front. Microbiol.

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Lyme disease is the most prominent tick-borne disease in the United States. Co-infections with the tick-transmitted pathogens Babesia microti and Borrelia burgdorferi sensu stricto are becoming a serious health problem. B. burgdorferi is an extracellular spirochete that causes Lyme disease while B. microti is a protozoan that infects erythrocytes and causes babesiosis. Testing of donated blood for Babesia species is not currently mandatory due to unavailability of an FDA approved test. Transmission of this protozoan by blood transfusion often results in high morbidity and mortality in recipients. Infection of C3H/HeJ mice with B. burgdorferi and B. microti individually results in inflammatory Lyme disease and display of human babesiosis-like symptoms, respectively. Here we use this mouse model to provide a detailed investigation of the reciprocal influence of the two pathogens on each other during co-infection. We show that B. burgdorferi infection attenuates parasitemia in mice while B. microti subverts the splenic immune response, such that a marked decrease in splenic B and T cells, reduction in antibody levels and diminished functional humoral immunity, as determined by spirochete opsonophagocytosis, are observed in co-infected mice compared to only B. burgdorferi infected mice. Furthermore, immunosuppression by B. microti in co-infected mice showed an association with enhanced Lyme disease manifestations. This study demonstrates the effect of only simultaneous infection by B. burgdorferi and B. microti on each pathogen, immune response and on disease manifestations with respect to infection by the spirochete and the parasite. In our future studies, we will examine the overall effects of sequential infection by these pathogens on host immune responses and disease outcomes.

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Section: Resultssupporting

confidence: 93%

Protozoan Parasite Babesia microti Subverts Adaptive Immunity and Enhances Lyme Disease Severity

Djokić¹,

Akoolo²,

Primus³

et al. 2019

Front. Microbiol.

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“…Recently, many kinds of cytokines secreting by CD4 + T cells was found produced by CD8 + cells [14]. And CD8 + T cell was reported to involve in the progress of S. japonicum infection [15].…”

Section: Introductionmentioning

confidence: 99%

Changes of CD103-expressing pulmonary CD4+ and CD8+ T cells in S. japonicum infected C57BL/6 mice

et al. 2019

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BackgroundRecent studies have shown that CD103 is an important marker for tissue-resident memory T cells (TRM) which plays an important role in anti-infection. However, the role of CD103+ TRM was not elucidated in the progress of S. japonicum infection induced disease.Methods6–8 weeks old C57BL/6 mice were infected by S. japonicum. Mice were sacrificed and the lungs were removed 5–6 weeks after infection. Immunofluorescent staining and Q-PCR were performed to identify the expression of CD103 molecule. Single cellular populations were made, percentages of CD103 on both CD4+ and CD8+ T lymphocytes were dynamical observed by flow cytometry (FCM). Moreover, the expression of memory T cells related molecules CD69 and CD62L, T cell function associated molecules CD107a, IFN-γ, IL-4, IL-9, and IL-10 were compared between CD103+ CD4+ and CD8+ T cells by FCM.ResultsCD103+ cells were emerged in the lung of both naive and S. japonicum infected mice. Both the percentage and the absolute numbers of pulmonary CD4+ and CD8+ cells were increased after S. japonicum infection (P < 0.05). The percentage of CD103+ cells in CD8+ T cells decreased significantly at the early stage of S. japonicum infection (P < 0.05). Increased CD69, decreased CD62L and CD107a expressions were detected on both CD4+ and CD8+ CD103+ T cells in the lungs of infected mice (P < 0.05). Compared to CD8+ CD103+ T cells, CD4+ CD103+ T cells from infected mice expressed higher level of CD69 and lower level CD62L molecules (P < 0.05). Moreover, higher percentage of IL-4+, IL-9+ and IL-10+ cells on CD4+ CD103+ pulmonary T cells was found in infected mice (P < 0.05). Significantly increased IL-4 and IL-9, and decreased IFN-γ expressing cells were detected in CD8+CD103+ cells of infected mice (P < 0.05).ConclusionsCD103-expressing pulmonary CD4+ and CD8+ T cells play important roles in mediating S. japonicum infection induced granulomatous inflammation in the lung.

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“…ULP treatments increased these organ indexes compared to the model group ( p < .05; Figure 13a). The thymus transports activated T cells into blood circulation and promotes the development of mast cell (Li et al., 2015). The spleen can synthesize the immune effector molecules and promotes the phagocytosis by granulocytes (Hassanpour, Joss, & Mohammad, 2013).…”

Section: Resultsmentioning

confidence: 99%

The algal polysaccharide ulvan suppresses growth of hepatoma cells

Zhao

Lin

et al. 2020

Food Frontiers

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Treatment for tumors depends on host immune system. The antitumor and immunoregulatory activities of Ulva lactuca polysaccharide (ULP) were evaluated in H22 tumorbearing mice and cyclophosphamide-induced immunosuppressed mice, respectively.The structural properties of ULP were identified through multi-angle laser light scattering, high-performance liquid chromatography, Fourier-transformed infrared, and nuclear magnetic resonance. It was composed of -D-Manp-(1→, →2,4)--L-Rhap-(1→, -D-GlcpA-(1→, -GalpA-(1→, →2,4)--D-Glcp-(1→, and →6)--D-Galp-(1→ with the molecular weight of 1.46 × 10 5 Da. Its antioxidant, anti-inflammatory, and antitumor effects were determined. Liver and tumor tissues were collected for histopathological, immunohistochemical, and western blotting analysis. ULP showed the great tumor growth inhibition of 74.41% compared with cyclophosphamide, which has side effects This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. c ○ 2020 The Authors. Food Frontiers published Food Frontiers. 2020;1:83-101. wileyonlinelibrary.com/journal/fft2 83 84 ZHAO ET AL. on immune system. ULP enhanced the expression of p53 to inhibit tumorigenesis, promoted the activation of IKK , and inhibited the activation of p65 within the NF-B pathway. ULP inhibited the tumor growth through downregulating the expressions of PI3K/Akt and mTOR, and promoting BAX/Bcl-2 ratio. The inhibition of TRAF2/TNF-and CD31/VEGF achieved a direct killing effect on tumor cells and inhibited tumor proliferation by inhibiting angiogenesis, respectively. Moreover, ULP increased the levels of immunoglobulin M and total superoxide dismutase, decreased the level of methane dicarboxylic aldehyde, and inhibited the activation of PI3K/AKT/mTOR/p70S6k pathways. The results showed that ULP exhibited pronounced antitumor activity and immunoregulatory effect. K E Y W O R D Santitumor, green alga, immunoregulation, structure, Ulva lactuca, ulvan

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The expression of molecule CD28 and CD38 on CD4+/CD8+ T lymphocytes in thymus and spleen elicited by Schistosoma japonicum infection in mice model

Cited by 11 publications

References 40 publications

Protozoan Parasite Babesia microti Subverts Adaptive Immunity and Enhances Lyme Disease Severity

Protozoan Parasite Babesia microti Subverts Adaptive Immunity and Enhances Lyme Disease Severity

Changes of CD103-expressing pulmonary CD4+ and CD8+ T cells in S. japonicum infected C57BL/6 mice

The algal polysaccharide ulvan suppresses growth of hepatoma cells

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