The expression of miRNA-146a-5p and its mechanism of treating dry eye syndrome

Abstract

Background: Dry eye syndrome in which tear fluid quality or abnormality or kinetic abnormality is caused by many causes, resulting in decreased tear film stability. In recent years, more and more results from the studies indicate that miRNA alterations are involved in dry eye syndrome. And miRNA-146a-5p is a key regulator to regulate the inflammatory response. In this paper, we demonstrated whether miRNA-146a-5p could cure dry eye syndrome by regulating target genes based on network analysis.Methods: In this paper, we collected the blood of patients with dry eye disease which served as a model group, the blood of healthy people was used as a control group. The expression of miRNA-146a-5p in the patients was detected by RT-PCR, the genes controlled by miRNA-146a-5p were predicted by Targetscan, miRDB, miRWalk and PicTar databases, the genes regulated by miRNA-146a-5p which is related to dry eye disease were selected by draw Venn diagram;Results: The comparison of the general information between patients and healthy people was no significant difference, and it indicated that the two groups were comparable. The results of databases showed that IRAK1 was one of the target genes regulated by miRNA-146a-5p and it is related to dry eye disease. The expression of miRNA-146a-5p was negatively related to IRAK1 mRNA and protein. While IRAK1 had positive correlation with IL-6, TNF-α and CBP proteins. Conclusion: These results emphasized that miRNA-146a-5p could inhibit the expression of IRAK1, IL-6, TNF-α and CBP so as to help reduce the inflammatory response in dry eye syndrome.