The Expression of miR-205 in Prostate Carcinoma and the Relationship with Prognosis in Patients

Abstract

Purpose. We aimed to investigate the changes of serum and cell exosome miR-205 levels in patients with prostate carcinoma and its clinical significance. Materials and Methods. Firstly, pronouncement of miR-205 in normal and prostate carcinoma tissues was analyzed by using UALCAN database. The relationship between miR-205 in tumor tissues and the pathological and clinical characteristics of patients with prostate carcinoma were analyzed. Consequently, 60 people with prostate carcinoma were collected to the Minhang Hospital from August 2016 to August 2021. Serum of patients in the two groups was collected, and RNA in serum exosomes was extracted, and qRT-PCR was used to analyze the expression of miR-205 mediated by serum exosomes. Meanwhile, the relationship among the clinical as well as pathological aspects and bodement of patients with prostate carcinoma and the pronouncement level of miR-205 mediated by exosome was compared. Next, assays like wound healing and CKK-8 were used to investigate the effects of miR-205 in exosomes extracted from prostate carcinoma on the augmentation and metastasis of prostate carcinoma. Results. The results showed that the pronouncement level of miR-205 in tissues with prostate carcinoma was significantly lower than that in normal prostate tissues. In addition, the pronouncement level of miR-205 in fluid exosome of people with prostate carcinoma and exosomes derived from the lines of prostate carcinoma was considerably less than that in serum exosomes of healthy patients and that of normal cell lines of prostate. The pronouncement level of miR-205 in fluid exosomes of people with prostate carcinoma was negatively associated with cancer phase, uncontrolled cell division in lymph nodes, distant metastasis, and PSA level at initial diagnosis. Analysis (multivariate and univariate) showed that miR-205 pronouncement was a sovereign threat cause for prognosis of prostate cancer patients. Additionally, the pronouncement and metastasis of prostate carcinoma can be restricted by the overexpression of miR-205. Conclusion. The pronouncement of miR-205 in liquid derived exosomes is correlated with the prediction of people with prostate carcinoma and may be a new marker for identification and cure of prostate carcinoma.

Systematic Analysis of Tissue‐Derived and Biofluid Extracellular Vesicle miRNAs Associated with Prostate Cancer

“…Table 1A represents the data from our EV literature search in PubMed, Embase, and Google Scholar. In our corpus of 31 articles, [ 9–13,17–42 ] miR‐21‐5p, miR‐141‐3p, and miR‐375‐3p were among the most represented (Table 1A). We cross‐referenced these data with Table 1B, which lists the dysregulated miRNA from our DESeq2 analysis (Figure 2).…”

CENPE and LDHA were potential prognostic biomarkers of chromophobe renal cell carcinoma

“…It had been reported that CNEPE and LDHA could be targeted by miRNA in some cancers [ 66 , 67 ]. What’s more, there was a wide regulatory spectrum between mRNA and miRNA, because the mRNA could bind to several miRNAs and the miRNA could bind to hundreds of mRNAs [ 68 ]. According to the four prediction datasets and expression datasets, we found hsa-miR-577 was the common target of CENPE and LDHA.…”

Recent Progress on Exosomes in the Diagnosis of Prostate Cancer

“…It is an advantage that the specimens of various types of body fluids can be applied, but the disadvantage is the cost of the instrument during operation, and requirement of large samples. Exosome sedimentation method (suitable for large samples, simple operation, but long cycle and low purity), immunoaffinity capture method (high purity, high cost, low yield), ExoQuick kits (the operation is simple and efficient but the use cost is high), ultrafiltration method (average purity and affected if blockage of the filter film) [39,80], has enormous potential but not yet developed a set of high efficiency, good specificity, low cost, and applicable to clinical standard protocols. The standard scheme is necessary to further explore the extraction solution of the exosome.…”